製品: DUSP1/MKP1 Antibody
カタログ: AF5286
タンパク質の説明: Rabbit polyclonal antibody to DUSP1/MKP1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 39 kDa; 39kD(Calculated).
ユニプロット: P28562
RRID: AB_2837772

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
クローナリティ:
Polyclonal
特異性:
DUSP1/MKP1 Antibody detects endogenous levels of total DUSP1/MKP1.
RRID:
AB_2837772
引用形式: Affinity Biosciences Cat# AF5286, RRID:AB_2837772.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

CL 100; CL100; Dual Specificity Phosphatase 1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; DUS1_HUMAN; DUSP 1; Dusp1; HVH1; MAP kinase phosphatase 1; Mitogen-activated protein kinase phosphatase 1; MKP-1; MKP1; Protein tyrosine phosphatase CL100; Protein-tyrosine phosphatase CL100; PTPN10; Serine/threonine specific protein phosphatase; VH1;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P28562 DUS1_HUMAN:

Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.

タンパク質の説明:
Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.
タンパク質配列:
MVMEVGTLDAGGLRALLGERAAQCLLLDCRSFFAFNAGHIAGSVNVRFSTIVRRRAKGAMGLEHIVPNAELRGRLLAGAYHAVVLLDERSAALDGAKRDGTLALAAGALCREARAAQVFFLKGGYEAFSASCPELCSKQSTPMGLSLPLSTSVPDSAESGCSSCSTPLYDQGGPVEILPFLYLGSAYHASRKDMLDALGITALINVSANCPNHFEGHYQYKSIPVEDNHKADISSWFNEAIDFIDSIKNAGGRVFVHCQAGISRSATICLAYLMRTNRVKLDEAFEFVKQRRSIISPNFSFMGQLLQFESQVLAPHCSAEAGSPAMAVLDRGTSTTTVFNFPVSIPVHSTNSALSYLQSPITTSPSC

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
88
Zebrafish
88
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P28562 基板として

Site PTM Type Enzyme
K57 Acetylation PR:000007102 (EP300)
K57 Ubiquitination
K97 Acetylation
K97 Ubiquitination
K122 Ubiquitination
S131 Phosphorylation
S140 Phosphorylation
T141 Phosphorylation
S146 Phosphorylation
K221 Ubiquitination
K230 Ubiquitination
K280 Ubiquitination
K289 Ubiquitination
S296 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1)
S323 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1)
S334 Phosphorylation P54646 (PRKAA2)
S359 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
S364 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1)

研究背景

機能:

Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.

PTMs:

Phosphorylation at Ser-359 and Ser-364 by MAPK1/ERK2 and MAPK3/ERK1 reduces its rate of degradation.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.

タンパク質ファミリー:

Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

研究領域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Serotonergic synapse.

参考文献

1). Prenatal co-exposure to diisodecyl phthalate and ozone contribute to depressive behavior in offspring mice through oxidative stress and TWIST1 participation. The Science of the total environment, 2024 (PubMed: 38608898) [IF=9.8]

2). Integrating scRNA and bulk-RNA sequencing develops a cell senescence signature for analyzing tumor heterogeneity in clear cell renal cell carcinoma. Frontiers in Immunology, 2023 (PubMed: 37503331) [IF=7.3]

Application: WB    Species: Human    Sample: 786-O cell

Figure 8 Construction of risk model and functional validation of critical genes. (A) Differential analysis between clust1 and no_clust1 in TCGA dataset; (B) Differential analysis between clust2 and no_clust2 in TCGA dataset; (C) Differential analysis between clust3 and no_clust3 in TCGA dataset; (D) A total of 961 promising candidates were identified among the differentially expressed genes; (E) Trajectory of each independent variable as lambda changes; (F) Confidence interval under lambda; (G) Coefficients of prognostic-related genes obtained from multivariate Cox analysis; (H) Western blot assay of DUSP1 protein expression in 786-O cell after transfection of Si-DUSP1; (I) Colony formation assay was carried out to evaluate the proliferation of 786-O cell; (J, K) Transwell assay was used to assess the migration and invasion of 786-O cell. (L) Western blot assay of p21 and p53 protein expression in 786-O cell after transfection of Si-DUSP1. *, p< 0.05; (M) SA-β-gal staining of 786-O cell. *, p< 0.05.

3). MKP-1 regulates the inflammatory activation of microglia against Alzheimer's disease. CNS Neuroscience & Therapeutics, 2023 (PubMed: 37602891) [IF=5.5]

Application: IHC    Species: Mouse    Sample:

FIGURE 3 MKP‐1 expression lowered in Alzheimer's disease (AD) model mice and AD model cells. (A) Immunofluorescence staining was performed in the brain of wild‐type (WT), AD mice. Iba1 (red) for microglia, green for MKP‐1, and 4′,6‐diamidino‐2‐phenylindole (DAPI) (blue) for nuclei. Scale bar: 50 μm. (B, C) Testing MKP‐1 positive area (%) and MKP‐1+ Iba1+/Iba1+ ratio in WT and AD mice. (D–F) Immunoblotting examined MKP‐1 and amyloid precursor protein (APP) protein levels in the brains of WT and AD mice. (G, H) Western blot analyses of BV2 microglia cell lines treated with amyloid‐β (Aβ) in a gradient level of 0, 2.5, 5, 10, and 20 μM. The results are displayed as the mean values ± SEM, n = 4 per group.

Application: WB    Species: Mouse    Sample:

FIGURE 3 MKP‐1 expression lowered in Alzheimer's disease (AD) model mice and AD model cells. (A) Immunofluorescence staining was performed in the brain of wild‐type (WT), AD mice. Iba1 (red) for microglia, green for MKP‐1, and 4′,6‐diamidino‐2‐phenylindole (DAPI) (blue) for nuclei. Scale bar: 50 μm. (B, C) Testing MKP‐1 positive area (%) and MKP‐1+ Iba1+/Iba1+ ratio in WT and AD mice. (D–F) Immunoblotting examined MKP‐1 and amyloid precursor protein (APP) protein levels in the brains of WT and AD mice. (G, H) Western blot analyses of BV2 microglia cell lines treated with amyloid‐β (Aβ) in a gradient level of 0, 2.5, 5, 10, and 20 μM. The results are displayed as the mean values ± SEM, n = 4 per group.

4). All-Trans Retinoic Acid Attenuates Blue Light-Induced Apoptosis of Retinal Photoreceptors by Upregulating MKP-1 Expression. Molecular Neurobiology, 2021 (PubMed: 33950345) [IF=5.1]

Application: WB    Species: Rat    Sample: retinas

Fig. 5 RA upregulates retinal expression of MKP-1 and inhibits JNK phosphorylation induced by light exposure. a, d Western blots of p-NF- κB/NF-κB, p-JNK/JNK and MKP-1 expression levels in the control and RA-treated groups unexposed to light and at 3 days and 7 days after light exposure. b, c, e Quantitative analysis of western blotting of the phosphorylation levels of NF-κB (b), JNK (c), and MKP-1 (e). Oneway ANOVA analysis followed by Dunnett’s test was used. n = 3 per group, *P < 0.05 and **P < 0.01. LI, light injury; RA, all-trans retinoic acid; JNK, c-Jun N-terminal kinase; NF-κB, nuclear factor-κB; MKP-1, mitogen-activated protein kinase phosphatase-1

5). Rosiglitazone Improves Glucocorticoid Resistance in a Sudden Sensorineural Hearing Loss by Promoting MAP Kinase Phosphatase-1 Expression. Mediators of Inflammation, 2019 (PubMed: 31217747) [IF=4.6]

Application: IF/ICC    Species: guinea pigs    Sample: spiral ganglion cell

Figure 3: |MKP-1 immunofluorescence staining of cochlear spiral ganglion of pigs in the AP group. MKP-1 stained in the spiral ganglion cell (white arrows). Blue: nuclei stained with DAPI;green: MKP-1 stained in inner hair cell (IHC) and outer hair cell (OHC). Scale bars = 10 μm. The red marker is the stereocilia of hair cells, and the blue marker is the cell nucleus.

Application: WB    Species: guinea pigs    Sample:

Figure 6:| The protein expression of (a) MKP-1 and GR, (b) p38 and p-p38, and (c) NF-κB and p-NF-κB p65 was determined by western blot analysis. Bar graphs show the quantification of the indicated proteins. Data are shown as means ± SD from at least three independent experiments, #p < 0 05 vs. AP group; ##p < 0 01 vs. AP group; ∗p < 0 05 vs. LPS+DEX group; ∗∗p < 0 01 vs. LPS+DEX group.

6). Exploration and validation of the hub genes involved in hypoxia-induced endothelial-mesenchymal transition of systemic sclerosis. Clinical and experimental rheumatology, 2023 (PubMed: 37246768) [IF=3.7]

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