CRHR1 Antibody - #AF5310

(1)  
製品: CRHR1 Antibody
カタログ: AF5310
タンパク質の説明: Rabbit polyclonal antibody to CRHR1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine
分子量: 51 kDa; 51kD(Calculated).
ユニプロット: P34998
RRID: AB_2837795

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(92%), Zebrafish(92%), Bovine(92%)
クローナリティ:
Polyclonal
特異性:
CRHR1 Antibody detects endogenous levels of total CRHR1.
RRID:
AB_2837795
引用形式: Affinity Biosciences Cat# AF5310, RRID:AB_2837795.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Corticotropin releasing factor type 1 receptor; corticotropin releasing hormone receptor 1; Corticotropin-releasing factor receptor 1; Corticotropin-releasing hormone receptor 1; CRF R; crf receptor 1; crf receptor type 1; crf type 1; CRF-R; CRF-R-1; CRF-R1; CRF1; CRFR; CRFR-1; CRFR1; CRFR1_HUMAN; CRH-R-1; CRH-R1; CRH-R1h; CRHR; Crhr1; CRHR1f; CRHR1L;

免疫原

免疫原:
Uniprot:

P34998(CRFR1_HUMAN) >>Visit HPA database.

遺伝子(ID):
CRHR1(104909134)
発現特異性:
P34998 CRFR1_HUMAN:

Predominantly expressed in the cerebellum, pituitary, cerebral cortex and olfactory lobe.

タンパク質の説明:
This is a receptor for corticotropin releasing factor. Shows high-affinity CRF binding. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
タンパク質配列:
MGGHPQLRLVKALLLLGLNPVSASLQDQHCESLSLASNISGLQCNASVDLIGTCWPRSPAGQLVVRPCPAFFYGVRYNTTNNGYRECLANGSWAARVNYSECQEILNEEKKSKVHYHVAVIINYLGHCISLVALLVAFVLFLRLRPGCTHWGDQADGALEVGAPWSGAPFQVRRSIRCLRNIIHWNLISAFILRNATWFVVQLTMSPEVHQSNVGWCRLVTAAYNYFHVTNFFWMFGEGCYLHTAIVLTYSTDRLRKWMFICIGWGVPFPIIVAWAIGKLYYDNEKCWFGKRPGVYTDYIYQGPMILVLLINFIFLFNIVRILMTKLRASTTSETIQYRKAVKATLVLLPLLGITYMLFFVNPGEDEVSRVVFIYFNSFLESFQGFFVSVFYCFLNSEVRSAIRKRWHRWQDKHSIRARVARAMSIPTSPTRVSFHSIKQSTAV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Bovine
92
Zebrafish
92
Xenopus
75
Chicken
75
Sheep
67
Rabbit
46
Horse
0
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P34998 基板として

Site PTM Type Enzyme
S330 Phosphorylation
S415 Phosphorylation
S425 Phosphorylation
S434 Phosphorylation
S437 Phosphorylation
S441 Phosphorylation
T442 Phosphorylation

研究背景

機能:

G-protein coupled receptor for CRH (corticotropin-releasing factor) and UCN (urocortin). Has high affinity for CRH and UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels. Inhibits the activity of the calcium channel CACNA1H. Required for normal embryonic development of the adrenal gland and for normal hormonal responses to stress. Plays a role in the response to anxiogenic stimuli.

PTMs:

C-terminal Ser or Thr residues may be phosphorylated.

Phosphorylation at Ser-330 by PKA prevents maximal coupling to Gq-protein, and thereby negatively regulates downstream signaling.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein. Endosome.
Note: Agonist-binding promotes endocytosis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Predominantly expressed in the cerebellum, pituitary, cerebral cortex and olfactory lobe.

サブユニット構造:

Heterodimer; heterodimerizes with GPER1 (By similarity). Interacts (via N-terminal extracellular domain) with CRH and UCN. Interacts with DLG1; this inhibits endocytosis of CRHR1 after agonist binding.

タンパク質ファミリー:

The transmembrane domain is composed of seven transmembrane helices that are arranged in V-shape. Transmembrane helix 7 assumes a sharply kinked structure. The antagonist CP-376395 binds at an allosteric site, far from the presumed binding site for the physiological peptide ligand.

Belongs to the G-protein coupled receptor 2 family.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Nervous system > Long-term depression.

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