製品: MCL1 Antibody
カタログ: AF5311
タンパク質の説明: Rabbit polyclonal antibody to MCL1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Rabbit, Dog, Chicken
分子量: 37 kDa, 50 kDa; 37kD(Calculated).
ユニプロット: Q07820
RRID: AB_2837796

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 100ul $280 在庫あり
 200ul $350 在庫あり

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(88%), Horse(100%), Rabbit(94%), Dog(100%), Chicken(91%)
クローナリティ:
Polyclonal
特異性:
MCL1 Antibody detects endogenous levels of total MCL1.
RRID:
AB_2837796
引用形式: Affinity Biosciences Cat# AF5311, RRID:AB_2837796.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Bcl 2 related protein EAT/mcl1; Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; BCL2 related; Bcl2-L-3; BCL2L3; EAT; Induced myeloid leukemia cell differentiation protein Mcl 1; Induced myeloid leukemia cell differentiation protein Mcl-1; MCL 1; MCL1; MCL1-ES; mcl1/EAT; MCL1_HUMAN; MCL1L; MCL1S; MGC104264; MGC1839; Myeloid Cell Leukemia 1; Myeloid cell leukemia ES; Myeloid cell leukemia sequence 1; Myeloid cell leukemia sequence 1 BCL2 related; Myeloid cell leukemia sequence 1 isoform 1; OTTHUMP00000032794; OTTHUMP00000032795; TM;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質の説明:
Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
タンパク質配列:
MFGLKRNAVIGLNLYCGGAGLGAGSGGATRPGGRLLATEKEASARREIGGGEAGAVIGGSAGASPPSTLTPDSRRVARPPPIGAEVPDVTATPARLLFFAPTRRAAPLEEMEAPAADAIMSPEEELDGYEPEPLGKRPAVLPLLELVGESGNNTSTDGSLPSTPPPAEEEEDELYRQSLEIISRYLREQATGAKDTKPMGRSGATSRKALETLRRVGDGVQRNHETAFQGMLRKLDIKNEDDVKSLSRVMIHVFSDGVTNWGRIVTLISFGAFVAKHLKTINQESCIEPLAESITDVLVRTKRDWLVKQRGWDGFVEFFHVEDLEGGIRNVLLAFAGVAGVGAGLAYLIR

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Rabbit
94
Chicken
91
Bovine
88
Xenopus
30
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q07820 基板として

Site PTM Type Enzyme
K5 Methylation
K5 Ubiquitination
K40 Ubiquitination
S64 Phosphorylation P45983 (MAPK8) , P24941 (CDK2) , P06493 (CDK1)
T68 Phosphorylation
T70 Phosphorylation
T92 Phosphorylation P06493 (CDK1) , P27361 (MAPK3) , P24941 (CDK2)
S121 Phosphorylation P45983 (MAPK8) , P24941 (CDK2) , P53779 (MAPK10)
Y129 Phosphorylation
K136 Ubiquitination
S150 Phosphorylation
T154 Phosphorylation
T156 Phosphorylation
S159 Phosphorylation P49840 (GSK3A) , P49841 (GSK3B)
S162 Phosphorylation
T163 Phosphorylation P24941 (CDK2) , P53779 (MAPK10) , P27361 (MAPK3) , P45983 (MAPK8) , P28482 (MAPK1)
K194 Ubiquitination
K197 Ubiquitination
K234 Ubiquitination
K238 Ubiquitination
K244 Ubiquitination
S245 Phosphorylation
K276 Ubiquitination
K279 Ubiquitination
K302 Ubiquitination
K308 Ubiquitination
Y347 Phosphorylation

研究背景

機能:

Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.

PTMs:

Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.

Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.

Phosphorylated on Ser-159, by GSK3, in response to IL3/interleukin-3 withdrawal. Phosphorylation at Ser-159 induces ubiquitination and proteasomal degradation, abrogating the anti-apoptotic activity. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.

Ubiquitinated. Ubiquitination is induced by phosphorylation at Ser-159.

細胞の位置付け:

Membrane>Single-pass membrane protein. Cytoplasm. Mitochondrion. Nucleus>Nucleoplasm.
Note: Cytoplasmic, associated with mitochondria.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with HIF3A (via C-terminus domain) (By similarity). Interacts with BAD, BOK, BIK and BMF (By similarity). Interacts with PMAIP1. Interacts with BBC3 (By similarity). Isoform 1 interacts with BAX, BAK1 and TPT1. Heterodimer of isoform 1 and isoform 2. Homodimers of isoform 1 or isoform 2 are not detected. Isoform 2 does not interact with pro-apoptotic BCL2-related proteins. Interacts with RTL10/BOP. Interacts with BCL2L11; may sequester BCL2L11 to prevent its pro-apoptotic activity. Interacts with GIMAP5 and HSPA8/HSC70; the interaction between HSPA8 and MCL1 is impaired in the absence of GIMAP5 (By similarity).

タンパク質ファミリー:

Belongs to the Bcl-2 family.

研究領域

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

参考文献

1). Novel-smoothened inhibitors for therapeutic targeting of naïve and drug-resistant hedgehog pathway-driven cancers. ACTA PHARMACOLOGICA SINICA, 2019 (PubMed: 29777201) [IF=8.2]

Application: WB    Species: mouse    Sample: NIH3T3 cells

Fig. 4|Targeting of Hh pathway function leads to downregulation of mTOR/AKT activity. a, b Serum-starved NIH3T3 cells were induced with SHH for 48h without or with the indicated inhibitors. Total cell lysates were prepared and subjected to immunoblotting as indicated. c293T cells were transfected with SMO-WT expression vector for 24h, treated with inhibitors for 48h, subjected to Annexin V-FITC/PI staining.Positive populations as analyzed by FACS are plotted. d Cells as in c were collected and immunoblotted

2). Enhancing the therapeutic efficacy of gefitinib on subcutaneously transplanted SKOV3 ovarian cancer tumors in nude mice via ultrasound‑stimulated microbubble cavitation. Experimental and therapeutic medicine, 2024 (PubMed: 39006449) [IF=2.7]

3). Association of CUL4B with the pathogenesis of age-related cataract. International ophthalmology, 2024 (PubMed: 38937308) [IF=1.6]

4). Molecular regulation of apoptotic machinery and lipid metabolism by mTORC1/mTORC2 dual inhibitors in preclinical models of HER2+/PIK3CAmut breast cancer. Oncotarget, 2016 (PubMed: 27563814)

Application: WB    Species: human    Sample:

Figure 4: The MTI-31-induced apoptosis requires Bim- and GSK3 activity. A and B. Cells of MDA-MB-453 (A) or MDAMB-231 (B) were treated as indicated. Total cell lysates were immunoblotted.

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