Cyclin A1 Antibody - #AF5313

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製品: Cyclin A1 Antibody
カタログ: AF5313
タンパク質の説明: Rabbit polyclonal antibody to Cyclin A1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat, Monkey
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 52 kDa; 52kD(Calculated).
ユニプロット: P78396
RRID: AB_2837798

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat,Monkey
予測:
Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(80%), Xenopus(82%)
クローナリティ:
Polyclonal
特異性:
Cyclin A1 Antibody detects endogenous levels of total Cyclin A1.
RRID:
AB_2837798
引用形式: Affinity Biosciences Cat# AF5313, RRID:AB_2837798.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CCN A1; CCNA 1; Ccna1; CCNA1_HUMAN; Cyclin-A1; CyclinA1; G2/mitotic specific cyclin A1; MGC132235; MGC159139;

免疫原

免疫原:
Uniprot:

P78396(CCNA1_HUMAN) >>Visit HPA database.

遺伝子(ID):
CCNA1(8900)
発現特異性:
P78396 CCNA1_HUMAN:

Very high levels in testis and very low levels in brain. Also found in myeloid leukemia cell lines.

タンパク質の説明:
May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells.
タンパク質配列:
METGFPAIMYPGSFIGGWGEEYLSWEGPGLPDFVFQQQPVESEAMHCSNPKSGVVLATVARGPDACQILTRAPLGQDPPQRTVLGLLTANGQYRRTCGQGITRIRCYSGSENAFPPAGKKALPDCGVQEPPKQGFDIYMDELEQGDRDSCSVREGMAFEDVYEVDTGTLKSDLHFLLDFNTVSPMLVDSSLLSQSEDISSLGTDVINVTEYAEEIYQYLREAEIRHRPKAHYMKKQPDITEGMRTILVDWLVEVGEEYKLRAETLYLAVNFLDRFLSCMSVLRGKLQLVGTAAMLLASKYEEIYPPEVDEFVYITDDTYTKRQLLKMEHLLLKVLAFDLTVPTTNQFLLQYLRRQGVCVRTENLAKYVAELSLLEADPFLKYLPSLIAAAAFCLANYTVNKHFWPETLAAFTGYSLSEIVPCLSELHKAYLDIPHRPQQAIREKYKASKYLCVSLMEPPAVLLLQ

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
83
Xenopus
82
Chicken
80
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P78396 基板として

Site PTM Type Enzyme
Ubiquitination
T168 Phosphorylation
S280 Phosphorylation

研究背景

機能:

May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells.

PTMs:

Polyubiquitinated via 'Lys-11'-linked ubiquitin by the anaphase-promoting complex (APC/C), leading to its degradation by the proteasome. Deubiquitinated and stabilized by USP37 enables entry into S phase.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Very high levels in testis and very low levels in brain. Also found in myeloid leukemia cell lines.

サブユニット構造:

Interacts with the CDK2 and the CDC2 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Does not bind CDK4 and CDK5 (in vitro). The cyclin A1-CDK2 complex interacts with transcription factor E2F-1 and RB proteins. Found in a complex with CDK2, CABLES1 and CCNE1 (By similarity). Interacts with INCA1. Interacts with KLHDC9.

タンパク質ファミリー:

Belongs to the cyclin family. Cyclin AB subfamily.

研究領域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

参考文献

1). PICH activates Cyclin A1 transcription to drive S-phase progression and chemoresistance in gastric cancer.. Cancer Research, 2023 (PubMed: 37646571) [IF=11.2]

Application: WB    Species: Human    Sample:

FigureS1.CyclinA1andA2arenotsuitableashistologicalpredictorsof5-FUresponseinGCpatients.A–D,ProteinexpressionofCyclinA1andCyclinA2inCyclinA1(CyclinA1OE)orA2(CyclinA2OE)overexpressingandknockingdown(shRNA1andshRNA2)AGS,MKN-45,NCI-N87 andSNU-216cellsdetectedbyImmunoblotting.E–H,PercentageofcellsindifferentphasesinCyclinA1OEorCyclinA2OEAGS,MKN-45,NCI-N87andSNU-216cellsdetectedbyFCM(n=3).I–K,Sensitivityto5-FUinCyclinA1OE,CyclinA2OEMKN-45,NCI-N87andSNU-216cells(n=4).L-O,Percentageofcellsindifferentphasesinknockingdown(shCyclinA1andshCyclinA2)AGS,MKN-45,NCI-N87andSNU-216cellsdetectedbyFCM(n=3).P-R,Sensitivityto5-FUinshRNA1andshRNA2MKN-45,NCI-N87andSNU-216cells(n=4).Alldataarethemean±SD.One-wayANOVA(G–L),
2). Silica nanoparticles cause spermatogenesis dysfunction in mice via inducing cell cycle arrest and apoptosis. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2022 (PubMed: 35051769) [IF=6.8]

Application: WB    Species: mouse    Sample: testis

Fig. 5. |SiNPs affected the protein expressions of cell cycle regulators. (A) The protein expressions of cyclin A1, CDK2, cyclin E1, CDK1, and cyclin B1 were significantly decreased in the testis after exposure to SiNPs.
3). STAT3 stimulates adipogenic stem cell proliferation and cooperates with HMGA2 during the early stage of differentiation to promote adipogenesis. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2017 (PubMed: 27940362) [IF=3.1]

Application: WB    Species: mouse    Sample:

STAT3 knockdown blocked cell cycle procession at S phase in adipogenic stem cells and early differentiating cells. (A) Flow cytometric analysis of 3T3-L1 stem cells at 48 h after siRNA introduction. Expression of cell cycle-associated genes was measured at mRNA (B) and protein (C) levels at 48 h after siRNA introduction in 3T3-L1 stem cells. (D) Flow cytometric analysis of 3T3-L1 cells that were differentiated for 24 h, with differentiation induced at 72 h after STAT3 siRNA introduction. qRT-PCR analysis (E) and Western blot analyses (F) of cell cycle-associated genes after STAT3 siRNA introduction. Values are presented as means ± S.D. of three replicates. * P < 0.01, # P < 0.05 vs siGL3.
4). Acrylamide-induced meiotic arrest of spermatocytes in adolescent mice by triggering excessive DNA strand breaks: Potential therapeutic effects of resveratrol. Human & experimental toxicology, 2023 (PubMed: 37550604) [IF=2.8]

Application: WB    Species: Mouse    Sample:

Figure 2. Effects of AA on proportions of testicular cell populations and expression of meiotic proteins. (a) Representative fluorescent cell sorting images of flow cytometric analysis of testicular cells. (b) The percentage of 1C, 2C and 4C testicular cells by flow cytometric analysis. (c) Representative immunofluorescence images of SYCP3 staining in the testis. (d) and (e) The expression levels of meiotic regulating factors (SYCP3, CDK2 and Cyclin A1) in the testis and isolated spermatocytes, respectively. The data are expressed as the mean ± SD of three mice in each group for annotations B and D, and are expressed as the mean ± SE of three separate experiments with triplicate samples for annotation E. *p < 0.05, **p < 0.01, compared with the control group.

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