PIAS4 Antibody - #AF5329
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(23)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF5329, RRID:AB_2837814.
折りたたみ／展開
E3 SUMO protein ligase PIAS4; E3 SUMO-protein ligase PIAS4; FLJ12419; MGC35296; PAIASgamma; PIAS 4; PIAS gamma; PIAS-gamma; Pias4; PIAS4_HUMAN; PIASG; PIASgamma; PIASy; Protein inhibitor of activated STAT 4; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma; Protein inhibitor of activated STAT protein PIASy; Zinc finger MIZ type containing 6; ZMIZ 6; ZMIZ6;
免疫原
Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes.
- Q8N2W9 PIAS4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAELVEAKNMVMSFRVSDLQMLLGFVGRSKSGLKHELVTRALQLVQFDCSPELFKKIKELYETRYAKKNSEPAPQPHRPLDPLTMHSTYDRAGAVPRTPLAGPNIDYPVLYGKYLNGLGRLPAKTLKPEVRLVKLPFFNMLDELLKPTELVPQNNEKLQESPCIFALTPRQVELIRNSRELQPGVKAVQVVLRICYSDTSCPQEDQYPPNIAVKVNHSYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSATNRITVTWGNYGKSYSVALYLVRQLTSSELLQRLKTIGVKHPELCKALVKEKLRLDPDSEIATTGVRVSLICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNEKKPTWMCPVCDKPAPYDQLIIDGLLSKILSECEDADEIEYLVDGSWCPIRAEKERSCSPQGAILVLGPSDANGLLPAPSVNGSGALGSTGGGGPVGSMENGKPGADVVDLTLDSSSSSEDEEEEEEEEEDEDEEGPRPKRRCPFQKGLVPAC
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8N2W9 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K9 | Sumoylation | Uniprot | |
| K9 | Ubiquitination | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| K35 | Sumoylation | Uniprot | |
| K35 | Ubiquitination | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| K59 | Sumoylation | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| K69 | Sumoylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| T99 | Phosphorylation | Uniprot | |
| Y108 | Phosphorylation | Uniprot | |
| K114 | Acetylation | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| K125 | Acetylation | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| K128 | Sumoylation | Uniprot | |
| K128 | Ubiquitination | Uniprot | |
| K135 | Sumoylation | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| K187 | Ubiquitination | Uniprot | |
| K231 | Sumoylation | Uniprot | |
| K231 | Ubiquitination | Uniprot | |
| Y274 | Phosphorylation | Uniprot | |
| K294 | Ubiquitination | Uniprot | |
| K300 | Ubiquitination | Uniprot | |
| K306 | Sumoylation | Uniprot | |
| K306 | Ubiquitination | Uniprot | |
| K330 | Ubiquitination | Uniprot | |
| K360 | Ubiquitination | Uniprot | |
| K411 | Ubiquitination | Uniprot | |
| K504 | Ubiquitination | Uniprot |
研究背景
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation.
Sumoylated. Lys-35 is the main site of sumoylation. Sumoylation is required for TCF4 sumoylation and transcriptional activation. Represses LEF1 transcriptional activity. SUMO1 is the preferred conjugate.
Nucleus>PML body.
Note: Colocalizes with SUMO1 and TCF7L2/TCF4 and LEF1 in a subset of PML (promyelocytic leukemia) nuclear bodies.
Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes.
Interacts with AR, AXIN1, GATA2, LEF1, TP53 and STAT1 (IFNG-induced). Binds to AT-rich DNA sequences, known as matrix or scaffold attachment regions (MARs/SARs) (By similarity). Interacts with TICAM1. Interacts with KLF8; the interaction results in SUMO ligation and repression of KLF8 transcriptional activity and of its cell cycle progression into G(1) phase. Interacts with MTA1.
The LXXLL motif is a coregulator signature that is essential for transcriptional corepression.
Belongs to the PIAS family.
研究領域
· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
参考文献
Application: WB Species: Human Sample: HK-2 cells
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