DNA PK Antibody - #AF5340

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製品: DNA PK Antibody
カタログ: AF5340
タンパク質の説明: Rabbit polyclonal antibody to DNA PK
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse
予測: Pig, Bovine, Sheep
分子量: 469 kDa; 469kD(Calculated).
ユニプロット: P78527
RRID: AB_2837825

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 100ul $280 在庫あり
 200ul $350 在庫あり

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(92%), Bovine(92%), Sheep(85%)
クローナリティ:
Polyclonal
特異性:
DNA PK Antibody detects endogenous levels of total DNA PK.
RRID:
AB_2837825
引用形式: Affinity Biosciences Cat# AF5340, RRID:AB_2837825.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

DNA dependent protein kinase catalytic subunit; DNA PK catalytic subunit; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; DNAPK; DNAPK catalytic subunit; DNPK 1; DNPK1; Hyper radiosensitivity of murine scid mutation, complementing 1; Hyperradiosensitivity complementing 1, mouse, homolog of 1; HYRC 1; HYRC; HYRC1; IMD26; p350; p460; PKRDC; PRKDC; PRKDC_HUMAN; Protein Kinase DNA Activated Catalytic Polypeptide; XRCC 7; XRCC7;

免疫原

免疫原:
Uniprot:

P78527(PRKDC_HUMAN) >>Visit HPA database.

遺伝子(ID):
PRKDC(5591)
タンパク質の説明:
Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties.
タンパク質配列:
MAGSGAGVRCSLLRLQETLSAADRCGAALAGHQLIRGLGQECVLSSSPAVLALQTSLVFSRDFGLLVFVRKSLNSIEFRECREEILKFLCIFLEKMGQKIAPYSVEIKNTCTSVYTKDRAAKCKIPALDLLIKLLQTFRSSRLMDEFKIGELFSKFYGELALKKKIPDTVLEKVYELLGLLGEVHPSEMINNAENLFRAFLGELKTQMTSAVREPKLPVLAGCLKGLSSLLCNFTKSMEEDPQTSREIFNFVLKAIRPQIDLKRYAVPSAGLRLFALHASQFSTCLLDNYVSLFEVLLKWCAHTNVELKKAALSALESFLKQVSNMVAKNAEMHKNKLQYFMEQFYGIIRNVDSNNKELSIAIRGYGLFAGPCKVINAKDVDFMYVELIQRCKQMFLTQTDTGDDRVYQMPSFLQSVASVLLYLDTVPEVYTPVLEHLVVMQIDSFPQYSPKMQLVCCRAIVKVFLALAAKGPVLRNCISTVVHQGLIRICSKPVVLPKGPESESEDHRASGEVRTGKWKVPTYKDYVDLFRHLLSSDQMMDSILADEAFFSVNSSSESLNHLLYDEFVKSVLKIVEKLDLTLEIQTVGEQENGDEAPGVWMIPTSDPAANLHPAKPKDFSAFINLVEFCREILPEKQAEFFEPWVYSFSYELILQSTRLPLISGFYKLLSITVRNAKKIKYFEGVSPKSLKHSPEDPEKYSCFALFVKFGKEVAVKMKQYKDELLASCLTFLLSLPHNIIELDVRAYVPALQMAFKLGLSYTPLAEVGLNALEEWSIYIDRHVMQPYYKDILPCLDGYLKTSALSDETKNNWEVSALSRAAQKGFNKVVLKHLKKTKNLSSNEAISLEEIRIRVVQMLGSLGGQINKNLLTVTSSDEMMKSYVAWDREKRLSFAVPFREMKPVIFLDVFLPRVTELALTASDRQTKVAACELLHSMVMFMLGKATQMPEGGQGAPPMYQLYKRTFPVLLRLACDVDQVTRQLYEPLVMQLIHWFTNNKKFESQDTVALLEAILDGIVDPVDSTLRDFCGRCIREFLKWSIKQITPQQQEKSPVNTKSLFKRLYSLALHPNAFKRLGASLAFNNIYREFREEESLVEQFVFEALVIYMESLALAHADEKSLGTIQQCCDAIDHLCRIIEKKHVSLNKAKKRRLPRGFPPSASLCLLDLVKWLLAHCGRPQTECRHKSIELFYKFVPLLPGNRSPNLWLKDVLKEEGVSFLINTFEGGGCGQPSGILAQPTLLYLRGPFSLQATLCWLDLLLAALECYNTFIGERTVGALQVLGTEAQSSLLKAVAFFLESIAMHDIIAAEKCFGTGAAGNRTSPQEGERYNYSKCTVVVRIMEFTTTLLNTSPEGWKLLKKDLCNTHLMRVLVQTLCEPASIGFNIGDVQVMAHLPDVCVNLMKALKMSPYKDILETHLREKITAQSIEELCAVNLYGPDAQVDRSRLAAVVSACKQLHRAGLLHNILPSQSTDLHHSVGTELLSLVYKGIAPGDERQCLPSLDLSCKQLASGLLELAFAFGGLCERLVSLLLNPAVLSTASLGSSQGSVIHFSHGEYFYSLFSETINTELLKNLDLAVLELMQSSVDNTKMVSAVLNGMLDQSFRERANQKHQGLKLATTILQHWKKCDSWWAKDSPLETKMAVLALLAKILQIDSSVSFNTSHGSFPEVFTTYISLLADTKLDLHLKGQAVTLLPFFTSLTGGSLEELRRVLEQLIVAHFPMQSREFPPGTPRFNNYVDCMKKFLDALELSQSPMLLELMTEVLCREQQHVMEELFQSSFRRIARRGSCVTQVGLLESVYEMFRKDDPRLSFTRQSFVDRSLLTLLWHCSLDALREFFSTIVVDAIDVLKSRFTKLNESTFDTQITKKMGYYKILDVMYSRLPKDDVHAKESKINQVFHGSCITEGNELTKTLIKLCYDAFTENMAGENQLLERRRLYHCAAYNCAISVICCVFNELKFYQGFLFSEKPEKNLLIFENLIDLKRRYNFPVEVEVPMERKKKYIEIRKEAREAANGDSDGPSYMSSLSYLADSTLSEEMSQFDFSTGVQSYSYSSQDPRPATGRFRRREQRDPTVHDDVLELEMDELNRHECMAPLTALVKHMHRSLGPPQGEEDSVPRDLPSWMKFLHGKLGNPIVPLNIRLFLAKLVINTEEVFRPYAKHWLSPLLQLAASENNGGEGIHYMVVEIVATILSWTGLATPTGVPKDEVLANRLLNFLMKHVFHPKRAVFRHNLEIIKTLVECWKDCLSIPYRLIFEKFSGKDPNSKDNSVGIQLLGIVMANDLPPYDPQCGIQSSEYFQALVNNMSFVRYKEVYAAAAEVLGLILRYVMERKNILEESLCELVAKQLKQHQNTMEDKFIVCLNKVTKSFPPLADRFMNAVFFLLPKFHGVLKTLCLEVVLCRVEGMTELYFQLKSKDFVQVMRHRDDERQKVCLDIIYKMMPKLKPVELRELLNPVVEFVSHPSTTCREQMYNILMWIHDNYRDPESETDNDSQEIFKLAKDVLIQGLIDENPGLQLIIRNFWSHETRLPSNTLDRLLALNSLYSPKIEVHFLSLATNFLLEMTSMSPDYPNPMFEHPLSECEFQEYTIDSDWRFRSTVLTPMFVETQASQGTLQTRTQEGSLSARWPVAGQIRATQQQHDFTLTQTADGRSSFDWLTGSSTDPLVDHTSPSSDSLLFAHKRSERLQRAPLKSVGPDFGKKRLGLPGDEVDNKVKGAAGRTDLLRLRRRFMRDQEKLSLMYARKGVAEQKREKEIKSELKMKQDAQVVLYRSYRHGDLPDIQIKHSSLITPLQAVAQRDPIIAKQLFSSLFSGILKEMDKFKTLSEKNNITQKLLQDFNRFLNTTFSFFPPFVSCIQDISCQHAALLSLDPAAVSAGCLASLQQPVGIRLLEEALLRLLPAELPAKRVRGKARLPPDVLRWVELAKLYRSIGEYDVLRGIFTSEIGTKQITQSALLAEARSDYSEAAKQYDEALNKQDWVDGEPTEAEKDFWELASLDCYNHLAEWKSLEYCSTASIDSENPPDLNKIWSEPFYQETYLPYMIRSKLKLLLQGEADQSLLTFIDKAMHGELQKAILELHYSQELSLLYLLQDDVDRAKYYIQNGIQSFMQNYSSIDVLLHQSRLTKLQSVQALTEIQEFISFISKQGNLSSQVPLKRLLNTWTNRYPDAKMDPMNIWDDIITNRCFFLSKIEEKLTPLPEDNSMNVDQDGDPSDRMEVQEQEEDISSLIRSCKFSMKMKMIDSARKQNNFSLAMKLLKELHKESKTRDDWLVSWVQSYCRLSHCRSRSQGCSEQVLTVLKTVSLLDENNVSSYLSKNILAFRDQNILLGTTYRIIANALSSEPACLAEIEEDKARRILELSGSSSEDSEKVIAGLYQRAFQHLSEAVQAAEEEAQPPSWSCGPAAGVIDAYMTLADFCDQQLRKEEENASVIDSAELQAYPALVVEKMLKALKLNSNEARLKFPRLLQIIERYPEETLSLMTKEISSVPCWQFISWISHMVALLDKDQAVAVQHSVEEITDNYPQAIVYPFIISSESYSFKDTSTGHKNKEFVARIKSKLDQGGVIQDFINALDQLSNPELLFKDWSNDVRAELAKTPVNKKNIEKMYERMYAALGDPKAPGLGAFRRKFIQTFGKEFDKHFGKGGSKLLRMKLSDFNDITNMLLLKMNKDSKPPGNLKECSPWMSDFKVEFLRNELEIPGQYDGRGKPLPEYHVRIAGFDERVTVMASLRRPKRIIIRGHDEREHPFLVKGGEDLRQDQRVEQLFQVMNGILAQDSACSQRALQLRTYSVVPMTSRLGLIEWLENTVTLKDLLLNTMSQEEKAAYLSDPRAPPCEYKDWLTKMSGKHDVGAYMLMYKGANRTETVTSFRKRESKVPADLLKRAFVRMSTSPEAFLALRSHFASSHALICISHWILGIGDRHLNNFMVAMETGGVIGIDFGHAFGSATQFLPVPELMPFRLTRQFINLMLPMKETGLMYSIMVHALRAFRSDPGLLTNTMDVFVKEPSFDWKNFEQKMLKKGGSWIQEINVAEKNWYPRQKICYAKRKLAGANPAVITCDELLLGHEKAPAFRDYVAVARGSKDHNIRAQEPESGLSEETQVKCLMDQATDPNILGRTWEGWEPWM

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Bovine
92
Sheep
85
Dog
77
Chicken
77
Rabbit
77
Xenopus
75
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P78527 基板として

Site PTM Type Enzyme
R14 Methylation
S20 Phosphorylation
R24 Methylation
C25 S-Nitrosylation
K71 Ubiquitination
K99 Ubiquitination
K108 Ubiquitination
C111 S-Nitrosylation
K117 Acetylation
K117 Ubiquitination
K124 Ubiquitination
T137 Phosphorylation
K148 Ubiquitination
K155 Ubiquitination
K163 Ubiquitination
K164 Ubiquitination
K165 Ubiquitination
K205 Ubiquitination
S210 Phosphorylation
K216 Ubiquitination
K225 Ubiquitination
K236 Ubiquitination
K254 Acetylation
K254 Ubiquitination
K263 Acetylation
K263 Ubiquitination
K310 Ubiquitination
S314 Phosphorylation
S318 Phosphorylation
K321 Ubiquitination
K329 Ubiquitination
K337 Ubiquitination
K357 Acetylation
K357 Ubiquitination
S360 Phosphorylation
C373 S-Nitrosylation
K374 Ubiquitination
K379 Ubiquitination
K393 Ubiquitination
T398 Phosphorylation
T400 Phosphorylation
K471 Ubiquitination
C478 S-Nitrosylation
S492 Phosphorylation
K493 Acetylation
K493 Ubiquitination
K499 Ubiquitination
S503 Phosphorylation
S505 Phosphorylation
S511 Phosphorylation Q96GD4 (AURKB)
T516 Phosphorylation
K518 Ubiquitination
K520 Ubiquitination
K525 Ubiquitination
Y527 Phosphorylation
S552 Phosphorylation
K574 Ubiquitination
Y667 Phosphorylation
K681 Acetylation
K681 Ubiquitination
Y682 Phosphorylation
S687 Phosphorylation
K689 Ubiquitination
K692 Ubiquitination
K700 Ubiquitination
K712 Ubiquitination
K717 Acetylation
Y721 Phosphorylation
Y779 Phosphorylation
K790 Ubiquitination
C795 S-Nitrosylation
K801 Ubiquitination
K810 Acetylation
K810 Ubiquitination
R820 Methylation
K824 Ubiquitination
K828 Acetylation
K828 Ubiquitination
K832 Acetylation
K832 Ubiquitination
K838 Ubiquitination
S841 Phosphorylation
S842 Phosphorylation
S847 Phosphorylation
S861 Phosphorylation
K868 Ubiquitination
S875 Phosphorylation
S876 Phosphorylation
K881 Ubiquitination
S882 Phosphorylation
Y883 Phosphorylation
K890 Ubiquitination
S893 Phosphorylation
K902 Ubiquitination
S922 Phosphorylation
T946 Phosphorylation P78527 (PRKDC)
K963 Ubiquitination
T1006 Phosphorylation P78527 (PRKDC)
K1038 Ubiquitination
K1042 Ubiquitination
K1051 Ubiquitination
S1052 Phosphorylation
K1057 Acetylation
K1057 Ubiquitination
Y1064 Phosphorylation
S1065 Phosphorylation
K1074 Acetylation
K1074 Ubiquitination
Y1086 Phosphorylation
K1140 Ubiquitination
K1141 Ubiquitination
K1147 Ubiquitination
K1186 Ubiquitination
S1187 Phosphorylation
K1193 Ubiquitination
K1209 Acetylation
K1209 Ubiquitination
T1275 Phosphorylation
K1311 Ubiquitination
C1312 S-Nitrosylation
K1334 Ubiquitination
K1357 Ubiquitination
K1360 Ubiquitination
K1361 Ubiquitination
K1407 Ubiquitination
K1412 Ubiquitination
K1422 Ubiquitination
K1456 Ubiquitination
S1470 Phosphorylation
T1481 Phosphorylation
K1489 Ubiquitination
C1499 S-Nitrosylation
C1507 S-Nitrosylation
S1545 Phosphorylation
S1546 Phosphorylation
S1594 Phosphorylation
K1612 Ubiquitination
K1617 Ubiquitination
T1620 Phosphorylation
T1621 Phosphorylation
K1627 Ubiquitination
K1628 Ubiquitination
K1635 Ubiquitination
K1642 Ubiquitination
S1726 Phosphorylation
T1733 Phosphorylation
K1744 Ubiquitination
S1790 Phosphorylation
T1793 Phosphorylation
K1807 Ubiquitination
S1841 Phosphorylation
T1842 Phosphorylation
T1856 Phosphorylation
K1857 Ubiquitination
S1861 Phosphorylation
K1869 Ubiquitination
K1870 Ubiquitination
K1875 Ubiquitination
K1886 Ubiquitination
K1892 Ubiquitination
K1895 Ubiquitination
K1913 Ubiquitination
K1917 Ubiquitination
K1970 Acetylation
K1970 Ubiquitination
K1973 Ubiquitination
K1985 Acetylation
K1985 Ubiquitination
K2003 Ubiquitination
S2023 Phosphorylation P78527 (PRKDC)
S2029 Phosphorylation P78527 (PRKDC)
S2034 Phosphorylation
S2041 Phosphorylation P78527 (PRKDC)
S2051 Phosphorylation
S2053 Phosphorylation P78527 (PRKDC)
S2056 Phosphorylation P78527 (PRKDC)
C2093 S-Nitrosylation
K2102 Ubiquitination
S2107 Phosphorylation
S2117 Phosphorylation
K2127 Ubiquitination
K2132 Ubiquitination
K2227 Ubiquitination
K2239 Methylation
K2239 Ubiquitination
K2246 Ubiquitination
S2250 Phosphorylation
K2259 Acetylation
K2259 Ubiquitination
K2263 Ubiquitination
K2313 Ubiquitination
Y2329 Phosphorylation
K2334 Ubiquitination
C2342 S-Nitrosylation
K2347 Ubiquitination
K2350 Ubiquitination
K2359 Acetylation
K2359 Ubiquitination
K2366 Ubiquitination
K2369 Ubiquitination
K2418 Ubiquitination
K2433 Ubiquitination
Y2440 Phosphorylation
K2441 Ubiquitination
K2445 Acetylation
K2445 Ubiquitination
K2447 Ubiquitination
K2500 Ubiquitination
K2503 Ubiquitination
T2535 Phosphorylation
Y2546 Phosphorylation
S2547 Phosphorylation
K2549 Sumoylation
K2549 Ubiquitination
S2599 Phosphorylation
T2600 Phosphorylation
T2603 Phosphorylation
T2609 Phosphorylation Q13535 (ATR) , P78527 (PRKDC) , Q13315 (ATM)
S2612 Phosphorylation Q13315 (ATM) , P78527 (PRKDC) , Q13535 (ATR)
T2615 Phosphorylation
T2618 Phosphorylation
T2620 Phosphorylation
S2624 Phosphorylation
S2626 Phosphorylation
T2638 Phosphorylation Q13315 (ATM) , P78527 (PRKDC)
T2645 Phosphorylation
T2647 Phosphorylation Q13315 (ATM) , Q13535 (ATR) , P78527 (PRKDC)
T2649 Phosphorylation
S2654 Phosphorylation
S2655 Phosphorylation
T2671 Phosphorylation
S2672 Phosphorylation
S2674 Phosphorylation
S2675 Phosphorylation
S2677 Phosphorylation
K2683 Acetylation
K2683 Ubiquitination
K2694 Acetylation
K2694 Methylation
K2694 Ubiquitination
S2695 Phosphorylation
K2702 Acetylation
K2702 Methylation
K2702 Ubiquitination
K2703 Methylation
K2703 Ubiquitination
R2704 Methylation
K2715 Ubiquitination
K2717 Ubiquitination
K2738 Acetylation
K2738 Ubiquitination
Y2743 Phosphorylation
K2746 Methylation
K2746 Ubiquitination
K2752 Ubiquitination
K2755 Ubiquitination
K2758 Sumoylation
K2758 Ubiquitination
K2764 Ubiquitination
K2786 Ubiquitination
S2789 Phosphorylation
T2792 Phosphorylation
K2806 Ubiquitination
S2814 Phosphorylation
K2818 Ubiquitination
K2824 Ubiquitination
S2827 Phosphorylation
K2829 Ubiquitination
K2835 Ubiquitination
K2908 Ubiquitination
K2928 Ubiquitination
S2932 Phosphorylation
Y2936 Phosphorylation
R2940 Methylation
K2950 Ubiquitination
S2955 Phosphorylation
K2970 Ubiquitination
K2978 Ubiquitination
T2987 Phosphorylation
K2991 Ubiquitination
S2998 Phosphorylation
S3010 Phosphorylation
C3014 S-Nitrosylation
S3018 Phosphorylation
S3021 Phosphorylation
K3050 Ubiquitination
K3067 Ubiquitination
K3075 Ubiquitination
K3128 Ubiquitination
K3147 Ubiquitination
K3158 Ubiquitination
R3167 Methylation
K3172 Ubiquitination
C3187 S-Nitrosylation
K3192 Ubiquitination
K3196 Ubiquitination
T3198 Phosphorylation
S3205 Phosphorylation P53350 (PLK1) , P78527 (PRKDC)
S3215 Phosphorylation
R3217 Methylation
S3233 Phosphorylation
K3235 Ubiquitination
K3239 Acetylation
K3239 Ubiquitination
K3241 Acetylation
K3241 Ubiquitination
K3248 Ubiquitination
S3253 Phosphorylation
K3257 Ubiquitination
K3260 Acetylation
K3260 Ubiquitination
K3264 Ubiquitination
K3267 Acetylation
S3290 Phosphorylation
C3293 S-Nitrosylation
S3294 Phosphorylation
K3302 Ubiquitination
S3314 Phosphorylation
Y3315 Phosphorylation
K3318 Ubiquitination
C3347 S-Nitrosylation
K3355 Acetylation
K3355 Ubiquitination
S3363 Phosphorylation
S3365 Phosphorylation
S3366 Phosphorylation
S3367 Phosphorylation
K3372 Methylation
K3372 Ubiquitination
Y3378 Phosphorylation
K3426 Ubiquitination
S3432 Phosphorylation
K3449 Ubiquitination
K3452 Ubiquitination
K3455 Ubiquitination
S3489 Phosphorylation
S3497 Phosphorylation
S3500 Phosphorylation
K3543 Ubiquitination
K3550 Ubiquitination
K3552 Acetylation
K3552 Ubiquitination
R3593 Methylation
K3598 Ubiquitination
T3599 Phosphorylation
K3608 Acetylation
K3608 Ubiquitination
K3621 Acetylation
K3621 Ubiquitination
K3631 Ubiquitination
K3638 Acetylation
K3638 Ubiquitination
K3642 Acetylation
K3642 Ubiquitination
K3646 Ubiquitination
K3650 Ubiquitination
K3655 Ubiquitination
S3657 Phosphorylation
T3663 Phosphorylation
K3669 Ubiquitination
K3672 Ubiquitination
K3675 Ubiquitination
K3681 Ubiquitination
K3691 Acetylation
K3691 Ubiquitination
R3696 Methylation
K3710 Ubiquitination
Y3715 Phosphorylation
S3731 Phosphorylation
K3753 Ubiquitination
S3779 Phosphorylation
Y3791 Phosphorylation
T3797 Phosphorylation
T3811 Phosphorylation
S3821 Phosphorylation
K3825 Ubiquitination
K3840 Acetylation
K3840 Ubiquitination
K3845 Ubiquitination
K3849 Ubiquitination
Y3855 Phosphorylation
Y3859 Phosphorylation
K3860 Ubiquitination
T3865 Phosphorylation
S3870 Phosphorylation
K3877 Ubiquitination
K3884 Ubiquitination
T3892 Phosphorylation
T3950 Phosphorylation P78527 (PRKDC)
S3993 Phosphorylation
K4007 Ubiquitination
K4014 Ubiquitination
K4019 Ubiquitination
K4023 Ubiquitination
S4026 Phosphorylation
K4036 Ubiquitination
K4043 Ubiquitination
C4045 S-Nitrosylation
K4048 Acetylation
K4048 Ubiquitination
K4050 Ubiquitination
K4070 Ubiquitination
R4075 Methylation
R4082 Methylation
S4084 Phosphorylation
K4085 Ubiquitination
S4096 Phosphorylation
K4105 Ubiquitination
C4106 S-Nitrosylation

PTMs - P78527 酵素として

Substrate Site Source
O43918 (AIRE) T68 Uniprot
O43918 (AIRE) S156 Uniprot
O96017 (CHEK2) T68 Uniprot
O96017 (CHEK2) T383 Uniprot
O96017 (CHEK2) T387 Uniprot
P04150-2 (NR3C1) S508 Uniprot
P04150-3 (NR3C1) S509 Uniprot
P04637 (TP53) S9 Uniprot
P04637-1 (TP53) S15 Uniprot
P04637 (TP53) T18 Uniprot
P04637-1 (TP53) S20 Uniprot
P04637-1 (TP53) S33 Uniprot
P04637-1 (TP53) S37 Uniprot
P04637 (TP53) S46 Uniprot
P05412 (JUN) S249 Uniprot
P07900 (HSP90AA1) T5 Uniprot
P07900 (HSP90AA1) T7 Uniprot
P07900-2 (HSP90AA1) T127 Uniprot
P07900-2 (HSP90AA1) T129 Uniprot
P07954 (FH) T236 Uniprot
P08670 (VIM) S56 Uniprot
P08670 (VIM) S430 Uniprot
P08670 (VIM) S459 Uniprot
P11831 (SRF) S435 Uniprot
P11831 (SRF) S446 Uniprot
P12956 (XRCC6) S6 Uniprot
P12956 (XRCC6) S27 Uniprot
P12956 (XRCC6) S33 Uniprot
P12956 (XRCC6) S51 Uniprot
P13010 (XRCC5) S577 Uniprot
P13010 (XRCC5) S580 Uniprot
P13010 (XRCC5) T715 Uniprot
P14859 (POU2F1) S78 Uniprot
P14859 (POU2F1) S81 Uniprot
P14859 (POU2F1) S85 Uniprot
P14859 (POU2F1) S88 Uniprot
P14859 (POU2F1) S92 Uniprot
P14859 (POU2F1) T100 Uniprot
P14859 (POU2F1) S141 Uniprot
P14859 (POU2F1) S143 Uniprot
P14859 (POU2F1) S147 Uniprot
P14859 (POU2F1) T162 Uniprot
P14859 (POU2F1) S167 Uniprot
P14859 (POU2F1) T226 Uniprot
P14859 (POU2F1) S232 Uniprot
P15927 (RPA2) S4 Uniprot
P15927-1 (RPA2) S8 Uniprot
P15927-1 (RPA2) S11 Uniprot
P15927-1 (RPA2) S12 Uniprot
P15927-1 (RPA2) S13 Uniprot
P15927-1 (RPA2) T21 Uniprot
P15927 (RPA2) S23 Uniprot
P15927 (RPA2) S29 Uniprot
P15927-1 (RPA2) S33 Uniprot
P16403 (HIST1H1C) T146 Uniprot
P17936 (IGFBP3) S183 Uniprot
P18887 (XRCC1) S371 Uniprot
P19838 (NFKB1) S20 Uniprot
P22415 (USF1) S262 Uniprot
P23025 (XPA) S173 Uniprot
P23025 (XPA) S196 Uniprot
P24928 (POLR2A) S1616 Uniprot
P24928 (POLR2A) S1621 Uniprot
P31749 (AKT1) T308 Uniprot
P31749 (AKT1) S473 Uniprot
P31751 (AKT2) T309 Uniprot
P31751 (AKT2) S474 Uniprot
P35637 (FUS) S26 Uniprot
P35637 (FUS) S42 Uniprot
P35637 (FUS) S61 Uniprot
P35637 (FUS) S84 Uniprot
P35637 (FUS) S131 Uniprot
P42575 (CASP2) S139 Uniprot
P43354 (NR4A2) S337 Uniprot
P49917 (LIG4) T650 Uniprot
P49917 (LIG4) S668 Uniprot
P49917 (LIG4) S672 Uniprot
P52945 (PDX1) T11 Uniprot
P55072 (VCP) S784 Uniprot
P55895 (RAG2) S365 Uniprot
P78527 (PRKDC) T946 Uniprot
P78527 (PRKDC) T1006 Uniprot
P78527 (PRKDC) S2023 Uniprot
P78527 (PRKDC) S2029 Uniprot
P78527 (PRKDC) S2041 Uniprot
P78527 (PRKDC) S2053 Uniprot
P78527 (PRKDC) S2056 Uniprot
P78527 (PRKDC) T2609 Uniprot
P78527 (PRKDC) S2612 Uniprot
P78527-1 (PRKDC) T2620 Uniprot
P78527-1 (PRKDC) S2624 Uniprot
P78527-1 (PRKDC) T2638 Uniprot
P78527-1 (PRKDC) T2647 Uniprot
P78527-1 (PRKDC) S3205 Uniprot
P78527 (PRKDC) T3950 Uniprot
Q00839 (HNRNPU) S59 Uniprot
Q13426 (XRCC4) S260 Uniprot
Q13426 (XRCC4) S304 Uniprot
Q13426 (XRCC4) S320 Uniprot
Q13426 (XRCC4) S327 Uniprot
Q13426 (XRCC4) S328 Uniprot
Q14191 (WRN) S319 Uniprot
Q14191 (WRN) S440 Uniprot
Q14191 (WRN) S467 Uniprot
Q14653 (IRF3) T135 Uniprot
Q15648 (MED1) T1457 Uniprot
Q15831 (STK11) T363 Uniprot
Q8TCS8 (PNPT1) S776 Uniprot
Q96SD1 (DCLRE1C) S503 Uniprot
Q96SD1 (DCLRE1C) S516 Uniprot
Q96SD1 (DCLRE1C) S534 Uniprot
Q96SD1 (DCLRE1C) S538 Uniprot
Q96SD1 (DCLRE1C) S548 Uniprot
Q96SD1 (DCLRE1C) S553 Uniprot
Q96SD1 (DCLRE1C) S645 Uniprot
Q96T60 (PNKP) S114 Uniprot
Q96T60 (PNKP) S126 Uniprot
Q9H4A6 (GOLPH3) T143 Uniprot
Q9H4A6 (GOLPH3) T148 Uniprot
Q9H9Q4 (NHEJ1) S55 Uniprot
Q9H9Q4 (NHEJ1) S132 Uniprot
Q9H9Q4 (NHEJ1) T223 Uniprot
Q9H9Q4 (NHEJ1) S245 Uniprot
Q9H9Q4 (NHEJ1) S251 Uniprot
Q9H9Q4 (NHEJ1) S263 Uniprot
Q9H9Q4 (NHEJ1) T266 Uniprot
Q9NUW8 (TDP1) S81 Uniprot

研究背景

機能:

Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, FH, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect mechanism (By similarity). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.

PTMs:

Autophosphorylated on Ser-2056, Thr-2609, Thr-2638 and Thr-2647. Ser-2056 and Thr-2609 are DNA damage-inducible phosphorylation sites (inducible with ionizing radiation, IR) dephosphorylated by PPP5C. Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair.

S-nitrosylated by GAPDH.

Polyubiquitinated by RNF144A, leading to proteasomal degradation.

細胞の位置付け:

Nucleus. Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

DNA-PK is a heterotrimer of PRKDC and the Ku p70/YRCC6-p86/XRCC5 dimer. Formation of this complex may be promoted by interaction with ILF3. Associates with the DNA-bound Ku heterodimer, but it can also bind to and be activated by free DNA. The DNA-PK heterotrimer associates with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex. Additional components of the NHEJ complex include NHEJ1/XLF and PAXX. Interacts with DNA-PKcs-interacting protein (KIP) with the region upstream the kinase domain. PRKDC alone also interacts with and phosphorylates DCLRE1C, thereby activating the latent endonuclease activity of this protein. Interacts with C1D. Interacts with TTI1 and TELO2. Interacts with CIB1. Interacts with SETX. Interacts with NR4A3; the DNA-dependent protein kinase complex DNA-PK phosphorylates and activates NR4A3 and prevents NR4A3 ubiquitination and degradation. Interacts with BRAT1. Part of the HDP-RNP complex composed of at least HEXIM1, PRKDC, XRCC5, XRCC6, paraspeckle proteins (SFPQ, NONO, PSPC1, RBM14, and MATR3) and NEAT1 RNA.

タンパク質ファミリー:

Belongs to the PI3/PI4-kinase family.

研究領域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Genetic Information Processing > Replication and repair > Non-homologous end-joining.

参考文献

1). Arsenic trioxide ameliorates experimental autoimmune encephalomyelitis in C57BL/6 mice by inducing CD4+ T cell apoptosis. Journal of Neuroinflammation, 2020 (PubMed: 32375831) [IF=9.3]
2). Suppression of fumarate hydratase activity increases the efficacy of cisplatin-mediated chemotherapy in gastric cancer. Cell Death & Disease, 2019 (PubMed: 31138787) [IF=9.0]

Application: WB    Species: human    Sample: SGC7901 and HGC27cells

Fig. 4| FH contributed to CDDP sensitivity via affecting DNA damage repair.g Immunoblotting for NHEJ pathway components in the indicated groups

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