FRA2 Antibody - #AF5345
(1)
(3)
製品説明
ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
FRA2 Antibody detects endogenous levels of total FRA2.
RRID:
AB_2837830
引用形式: Affinity Biosciences Cat# AF5345, RRID:AB_2837830.
引用形式: Affinity Biosciences Cat# AF5345, RRID:AB_2837830.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:
折りたたみ／展開
FLJ23306; Fos L2; FOS like antigen 2; Fos related antigen 2; Fos-related antigen 2; FosL 2; Fosl2; FOSL2_HUMAN; FRA 2; FRA-2;
免疫原
免疫原:
Uniprot:
遺伝子(ID):
タンパク質の説明:
Controls osteoclast survival and size (By similarity). As a dimer with JUN, activates LIF transcription (By similarity). Activates CEBPB transcription in PGE2-activated osteoblasts.
タンパク質配列:
- P15408 FOSL2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MYQDYPGNFDTSSRGSSGSPAHAESYSSGGGGQQKFRVDMPGSGSAFIPTINAITTSQDLQWMVQPTVITSMSNPYPRSHPYSPLPGLASVPGHMALPRPGVIKTIGTTVGRRRRDEQLSPEEEEKRRIRRERNKLAAAKCRNRRRELTEKLQAETEELEEEKSGLQKEIAELQKEKEKLEFMLVAHGPVCKISPEERRSPPAPGLQPMRSGGGSVGAVVVKQEPLEEDSPSSSSAGLDKAQRSVIKPISIAGGFYGEEPLHTPIVVTSTPAVTPGTSNLVFTYPSVLEQESPASPSESCSKAHRRSSSSGDQSSDSLNSPTLLAL
種類予測
種類予測:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P15408 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| Y2 | Phosphorylation | Uniprot | |
| Y5 | Phosphorylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| S17 | Phosphorylation | Uniprot | |
| S19 | Phosphorylation | Uniprot | |
| K35 | Ubiquitination | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| S83 | Phosphorylation | Uniprot | |
| K104 | Acetylation | Uniprot | |
| K104 | Methylation | Uniprot | |
| K104 | Ubiquitination | Uniprot | |
| S120 | Phosphorylation | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| K168 | Ubiquitination | Uniprot | |
| S194 | Phosphorylation | Uniprot | |
| S200 | Phosphorylation | Uniprot | |
| S211 | Phosphorylation | Uniprot | |
| S215 | Phosphorylation | Uniprot | |
| K222 | Acetylation | Uniprot | |
| K222 | Sumoylation | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| S235 | Phosphorylation | Uniprot | |
| K240 | Acetylation | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| S307 | Phosphorylation | Uniprot | |
| S308 | Phosphorylation | Uniprot | |
| S309 | Phosphorylation | Uniprot | |
| S310 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| S320 | Phosphorylation | Uniprot | |
| T322 | Phosphorylation | Uniprot |
研究背景
機能:
Controls osteoclast survival and size. As a dimer with JUN, activates LIF transcription. Activates CEBPB transcription in PGE2-activated osteoblasts.
細胞の位置付け:
Nucleus.
サブユニット構造:
Heterodimer.
タンパク質ファミリー:
Belongs to the bZIP family. Fos subfamily.
研究領域
· Organismal Systems > Development > Osteoclast differentiation. (View pathway)
参考文献
1). FOSL2 participates in renal fibrosis via SGK1-mediated epithelial-mesenchymal transition of proximal tubular epithelial cells. Journal of Translational Internal Medicine, 2023
(PubMed: 37662889)
[IF=4.9]
Application: WB Species: Mouse Sample: kidneys
Figure 2 The expression level of FOSL2 is elevated in UUO-injured kidneys. (A) Schematic diagram of UUO and adenovirus infection experiments in mice. (B) Western blotting analyses of protein expression levels of α-SMA, fibronectin, and collagen type I at days 3, 7, and 14. (C) Immunohistochemistry was conducted with anti-FOSL2 antibody to demonstrate FOSL2 protein expression in renal tissues. Magnification: 400×. Scale bar: 50 μm. (D) The mRNA and protein expression levels of FOSL2 were analyzed using qRT-PCR and western blotting, respectively. The values are expressed as mean ± standard deviation. *P < 0.05 versus sham (Day 3) group; ##P < 0.01 versus sham (Day 7) group, ++P < 0.01 versus sham (Day 14) group. UUO: unilateral ureteral obstruction; α-SMA: α-smooth muscle actin; qRT-PCR: quantitative real-time polymerase chain reaction; FOSL2: fos-related antigen 2.
Application: IHC Species: Mouse Sample: kidneys
Figure 2 The expression level of FOSL2 is elevated in UUO-injured kidneys. (A) Schematic diagram of UUO and adenovirus infection experiments in mice. (B) Western blotting analyses of protein expression levels of α-SMA, fibronectin, and collagen type I at days 3, 7, and 14. (C) Immunohistochemistry was conducted with anti-FOSL2 antibody to demonstrate FOSL2 protein expression in renal tissues. Magnification: 400×. Scale bar: 50 μm. (D) The mRNA and protein expression levels of FOSL2 were analyzed using qRT-PCR and western blotting, respectively. The values are expressed as mean ± standard deviation. *P < 0.05 versus sham (Day 3) group; ##P < 0.01 versus sham (Day 7) group, ++P < 0.01 versus sham (Day 14) group. UUO: unilateral ureteral obstruction; α-SMA: α-smooth muscle actin; qRT-PCR: quantitative real-time polymerase chain reaction; FOSL2: fos-related antigen 2.
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