HDAC5 Antibody - #AF5348

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製品: HDAC5 Antibody
カタログ: AF5348
タンパク質の説明: Rabbit polyclonal antibody to HDAC5
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Sheep, Rabbit, Dog
分子量: 122 kDa; 122kD(Calculated).
ユニプロット: Q9UQL6
RRID: AB_2837833

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(93%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
HDAC5 Antibody detects endogenous levels of total HDAC5.
RRID:
AB_2837833
引用形式: Affinity Biosciences Cat# AF5348, RRID:AB_2837833.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Antigen NY CO 9; Antigen NY-CO-9; HD5; HDAC 5; HDAC5; HDAC5_HUMAN; Histone deacetylase 5; NY CO 9;

免疫原

免疫原:
Uniprot:

Q9UQL6(HDAC5_HUMAN) >>Visit HPA database.

遺伝子(ID):
HDAC5(10014)
発現特異性:
Q9UQL6 HDAC5_HUMAN:

Ubiquitous.

タンパク質の説明:
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events.
タンパク質配列:
MNSPNESDGMSGREPSLEILPRTSLHSIPVTVEVKPVLPRAMPSSMGGGGGGSPSPVELRGALVGSVDPTLREQQLQQELLALKQQQQLQKQLLFAEFQKQHDHLTRQHEVQLQKHLKQQQEMLAAKQQQEMLAAKRQQELEQQRQREQQRQEELEKQRLEQQLLILRNKEKSKESAIASTEVKLRLQEFLLSKSKEPTPGGLNHSLPQHPKCWGAHHASLDQSSPPQSGPPGTPPSYKLPLPGPYDSRDDFPLRKTASEPNLKVRSRLKQKVAERRSSPLLRRKDGTVISTFKKRAVEITGAGPGASSVCNSAPGSGPSSPNSSHSTIAENGFTGSVPNIPTEMLPQHRALPLDSSPNQFSLYTSPSLPNISLGLQATVTVTNSHLTASPKLSTQQEAERQALQSLRQGGTLTGKFMSTSSIPGCLLGVALEGDGSPHGHASLLQHVLLLEQARQQSTLIAVPLHGQSPLVTGERVATSMRTVGKLPRHRPLSRTQSSPLPQSPQALQQLVMQQQHQQFLEKQKQQQLQLGKILTKTGELPRQPTTHPEETEEELTEQQEVLLGEGALTMPREGSTESESTQEDLEEEDEEDDGEEEEDCIQVKDEEGESGAEEGPDLEEPGAGYKKLFSDAQPLQPLQVYQAPLSLATVPHQALGRTQSSPAAPGGMKSPPDQPVKHLFTTGVVYDTFMLKHQCMCGNTHVHPEHAGRIQSIWSRLQETGLLSKCERIRGRKATLDEIQTVHSEYHTLLYGTSPLNRQKLDSKKLLGPISQKMYAVLPCGGIGVDSDTVWNEMHSSSAVRMAVGCLLELAFKVAAGELKNGFAIIRPPGHHAEESTAMGFCFFNSVAITAKLLQQKLNVGKVLIVDWDIHHGNGTQQAFYNDPSVLYISLHRYDNGNFFPGSGAPEEVGGGPGVGYNVNVAWTGGVDPPIGDVEYLTAFRTVVMPIAHEFSPDVVLVSAGFDAVEGHLSPLGGYSVTARCFGHLTRQLMTLAGGRVVLALEGGHDLTAICDASEACVSALLSVELQPLDEAVLQQKPNINAVATLEKVIEIQSKHWSCVQKFAAGLGRSLREAQAGETEEAETVSAMALLSVGAEQAQAAAAREHSPRPAEEPMEQEPAL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
93
Xenopus
77
Zebrafish
69
Pig
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UQL6 基板として

Site PTM Type Enzyme
S3 Phosphorylation
S7 Phosphorylation
K35 Sumoylation
S53 Phosphorylation
S55 Phosphorylation
S66 Phosphorylation
T70 Phosphorylation
K84 Ubiquitination
K115 Ubiquitination
K136 Ubiquitination
S180 Phosphorylation
T181 Phosphorylation
S206 Phosphorylation
S220 Phosphorylation
T234 Phosphorylation
K256 Ubiquitination
S259 Phosphorylation O43318 (MAP3K7) , Q05655 (PRKCD) , Q13555 (CAMK2G) , Q7KZI7 (MARK2) , Q9UQM7 (CAMK2A) , Q16566 (CAMK4) , P57059 (SIK1) , Q15139 (PRKD1) , Q13131 (PRKAA1) , Q14012 (CAMK1) , P54646 (PRKAA2)
S278 Phosphorylation Q96GD4 (AURKB)
S279 Phosphorylation Q9Y463 (DYRK1B) , P17612 (PRKACA) , Q00535 (CDK5)
T288 Phosphorylation
S291 Phosphorylation
T292 Phosphorylation Q16512 (PKN1)
S368 Phosphorylation
S469 Phosphorylation
T496 Phosphorylation
S498 Phosphorylation P54646 (PRKAA2) , Q14012 (CAMK1) , Q16566 (CAMK4) , Q13555 (CAMK2G) , Q13557 (CAMK2D) , Q9UQM7 (CAMK2A) , P57059 (SIK1) , Q15139 (PRKD1) , O94806 (PRKD3) , Q13131 (PRKAA1) , Q9BZL6 (PRKD2)
S504 Phosphorylation
K533 Acetylation
K533 Sumoylation
K537 Ubiquitination
T546 Phosphorylation
T547 Phosphorylation
K605 Sumoylation
S611 Phosphorylation
S661 Phosphorylation
S671 Phosphorylation
S755 Phosphorylation
Y776 Phosphorylation
S798 Phosphorylation
S799 Phosphorylation
S1071 Phosphorylation
S1108 Phosphorylation

研究背景

機能:

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Serves as a corepressor of RARA and causes its deacetylation. In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response.

PTMs:

Phosphorylated by AMPK, CaMK1, SIK1 and PRKD1 at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm and inhibition. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. Phosphorylated by GRK5, leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription (By similarity).

Ubiquitinated. Polyubiquitination however does not lead to its degradation.

細胞の位置付け:

Nucleus. Cytoplasm.
Note: Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by AMPK, CaMK1 and SIK1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitous.

サブユニット構造:

Interacts with AHRR, BAHD1, BCOR, HDAC7, HDAC9, CTBP1, MEF2C, NCOR2, NRIP1, PHB2 and a 14-3-3 chaperone protein. Interacts with BCL6, DDIT3/CHOP, GRK5, KDM5B and MYOCD. Interacts with EP300 in the presence of TFAP2C. Interacts with ANKRA2. Interacts with CUL7 (as part of the 3M complex); negatively regulated by ANKRA2. Interacts with ZBTB7B; the interaction allows the recruitment of HDAC4 on CD8 loci for deacetylation and possible inhibition of CD8 genes expression (By similarity). Interacts with RARA.

タンパク質ファミリー:

The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.

Belongs to the histone deacetylase family. HD type 2 subfamily.

研究領域

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

参考文献

1). WNT10A induces apoptosis of senescent synovial resident stem cells through Wnt/calcium pathway-mediated HDAC5 phosphorylation in OA joints. BONE, 2021 (PubMed: 34000432) [IF=4.1]

Application: IHC    Species: human    Sample: Sn-SMSCs

Fig. 4. |Histone deacetylase 5 (HDAC5) is involved in WNT10A-induced apoptosis of Sn-SMSCs: B: Immunohistochemistry staining for HDAC5 (scale bars = 50 μm) and caspase3 (scale bars = 100 μm) after 0/1/24/72 h of culture in complete medium supplemented with 50 ng/mL WNT10A(n = 5).

Application: WB    Species: human    Sample: Sn-SMSCs

Fig. 4. |Histone deacetylase 5 (HDAC5) is involved in WNT10A-induced apoptosis of Sn-SMSCs: C: HDAC5 mRNA (left), protein (middle) levels. and HDAC5 phosphorylation at s498 (right); *P<0.05

Application: IHC    Species: Rat    Sample: senescent cells (SnCs)

Fig. 4. Histone deacetylase 5 (HDAC5) is involved in WNT10A-induced apoptosis of Sn-SMSCs: A: RNA-seq results after 3 days of culture in complete medium supplemented with PBS (control) or 50 ng/mL WNT10A (n = 3). A: Top 10 hub proteins (left) and PPI (right). B: Immunohistochemistry staining for HDAC5 (scale bars = 50 μ m) and caspase3 (scale bars = 100 μ m) after 0/1/ 24/72 h of culture in complete medium supplemented with 50 ng/mL WNT10A (n = 5). C: HDAC5 mRNA (left), protein (middle) levels. and HDAC5 phos- phorylation at s498 (right); *P<0.05.

Application: WB    Species: Rat    Sample: senescent cells (SnCs)

Fig. 4. Histone deacetylase 5 (HDAC5) is involved in WNT10A-induced apoptosis of Sn-SMSCs: A: RNA-seq results after 3 days of culture in complete medium supplemented with PBS (control) or 50 ng/mL WNT10A (n = 3). A: Top 10 hub proteins (left) and PPI (right). B: Immunohistochemistry staining for HDAC5 (scale bars = 50 μ m) and caspase3 (scale bars = 100 μ m) after 0/1/ 24/72 h of culture in complete medium supplemented with 50 ng/mL WNT10A (n = 5). C: HDAC5 mRNA (left), protein (middle) levels. and HDAC5 phos- phorylation at s498 (right); *P<0.05.

Application: IF/ICC    Species: Rat    Sample: senescent cells (SnCs)

Fig. 5. WNT10A phosphorylates HDAC5 via the Wnt/calcium pathway: A: Expression of β-catenin and GSK3β (left, upper); expression of p-JNK and JNK (left, lower); expression of p-PKC and PKC, p-CaMKII and CaMKII, p-NFAT, and NFAT (middle); ratio of p-PKC:PKC, p-CaMKII:CaMKII and p-NFAT:NFAT (right) after 24 h of culture in complete medium supplemented with PBS (control) or 50 ng/mL WNT10A. B: Measurement of intracellular Ca2+ concentration using Fluo-4 AM. C: Expression of p-CaMKII and CaMKII, p-HDAC5 s498 and HDAC5, and p53 and acetylation p53 at K120 (upper); ratio of p-CaMKII:CaMKII and p-HDAC5 s498:HDAC5 (lower) after 24 h of culture in complete medium supplemented with PBS (control), 50 ng/mL WNT10A, or 50 ng/mL WNT10A + 10 μ M KN-62 (an inhibitor of CaMKII) (n = 5). D: Immunofluorescence staining for HDAC5 after 24 h of culture in complete medium supplemented with PBS (control), 50 ng/mL WNT10A, or 50 ng/mL WNT10A + 10 μ M KN-62 (n = 5); scale bars = 50 μ m; *P<0.05, **P<0.01.

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