ACTG2 Antibody - #AF5351
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF5351, RRID:AB_2837836.
折りたたみ／展開
ACT; ACTA3; ACTE; ACTG2; ACTH_HUMAN; Actin; Actin gamma 2 smooth muscle enteric; Actin gamma enteric smooth muscle; Actin like protein; ACTL3; ACTSG; Alpha actin 3; Alpha-actin-3; Gamma 2 actin; Gamma-2-actin; gamma-enteric smooth muscle; Smooth muscle gamma actin; Smooth muscle gamma-actin;
免疫原
- P63267 ACTH_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MCEEETTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKPEYDEAGPSIVHRKCF
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P63267 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| E3 | Acetylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| K51 | Acetylation | Uniprot | |
| K51 | Methylation | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| Y54 | Phosphorylation | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| K62 | Acetylation | Uniprot | |
| K62 | Sumoylation | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| T67 | Phosphorylation | Uniprot | |
| K69 | Acetylation | Uniprot | |
| K69 | Methylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| Y70 | Phosphorylation | Uniprot | |
| T78 | Phosphorylation | Uniprot | |
| K85 | Acetylation | Uniprot | |
| K85 | Methylation | Uniprot | |
| S90 | Phosphorylation | Uniprot | |
| Y92 | Phosphorylation | Uniprot | |
| R96 | Methylation | Uniprot | |
| K114 | Acetylation | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| T127 | Phosphorylation | Uniprot | |
| Y144 | Phosphorylation | Uniprot | |
| T150 | Phosphorylation | Uniprot | |
| S156 | Phosphorylation | Uniprot | |
| Y167 | Phosphorylation | Uniprot | |
| Y170 | Phosphorylation | Uniprot | |
| T187 | Phosphorylation | Uniprot | |
| Y189 | Phosphorylation | Uniprot | |
| K192 | Acetylation | Uniprot | |
| K192 | Methylation | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| T195 | Phosphorylation | Uniprot | |
| R197 | Methylation | Uniprot | |
| Y199 | Phosphorylation | Uniprot | |
| S200 | Phosphorylation | Uniprot | |
| T203 | Phosphorylation | Uniprot | |
| K214 | Acetylation | Uniprot | |
| K214 | Ubiquitination | Uniprot | |
| K216 | Acetylation | Uniprot | |
| K216 | Methylation | Uniprot | |
| Y219 | Phosphorylation | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| S235 | Phosphorylation | Uniprot | |
| S236 | Phosphorylation | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| S240 | Phosphorylation | Uniprot | |
| Y241 | Phosphorylation | Uniprot | |
| T250 | Phosphorylation | Uniprot | |
| T261 | Phosphorylation | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| T279 | Phosphorylation | Uniprot | |
| Y280 | Phosphorylation | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| Y295 | Phosphorylation | Uniprot | |
| T304 | Phosphorylation | Uniprot | |
| T305 | Phosphorylation | Uniprot | |
| Y307 | Phosphorylation | Uniprot | |
| K316 | Acetylation | Uniprot | |
| K316 | Sumoylation | Uniprot | |
| K316 | Ubiquitination | Uniprot | |
| T319 | Phosphorylation | Uniprot | |
| S324 | Phosphorylation | Uniprot | |
| T325 | Phosphorylation | Uniprot | |
| K327 | Acetylation | Uniprot | |
| K327 | Methylation | Uniprot | |
| K327 | Sumoylation | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| K329 | Acetylation | Uniprot | |
| K329 | Sumoylation | Uniprot | |
| K329 | Ubiquitination | Uniprot | |
| K337 | Sumoylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| Y363 | Phosphorylation | Uniprot |
研究背景
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Oxidation of Met-45 and Met-48 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization.
Monomethylation at Lys-85 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.
Methylated at His-74 by SETD3.
(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-51 of one monomer and Glu-271 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.
Cytoplasm>Cytoskeleton.
Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.
Belongs to the actin family.
研究領域
· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)
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