p18 INK4c Antibody - #AF5356

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製品: p18 INK4c Antibody
カタログ: AF5356
タンパク質の説明: Rabbit polyclonal antibody to p18 INK4c
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat, Monkey
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 18 kDa; 18kD(Calculated).
ユニプロット: P42773
RRID: AB_2837841

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MS Report
HPLC Report
MSDS
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IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat,Monkey
予測:
Pig(91%), Bovine(91%), Horse(82%), Sheep(91%), Rabbit(82%), Dog(91%)
クローナリティ:
Polyclonal
特異性:
p18 INK4c Antibody detects endogenous levels of total p18 INK4c.
RRID:
AB_2837841
引用形式: Affinity Biosciences Cat# AF5356, RRID:AB_2837841.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CDK6 inhibitor p18; CDKN 2C; CDKN 6; Cdkn2c; CDKN6; CDN2C_HUMAN; Cyclin dependent inhibitor; Cyclin dependent kinase 4 inhibitor C; Cyclin dependent kinase 6 inhibitor; Cyclin dependent kinase 6 inhibitor p18; Cyclin dependent kinase inhibitor 2C (p18 inhibits CDK4); Cyclin dependent kinase inhibitor 2C; Cyclin-dependent kinase 4 inhibitor C; Cyclin-dependent kinase 6 inhibitor; INK4C; OTTHUMP00000046546; p18; p18 inhibits CDK4; p18 INK4c; p18 INK6; p18-INK4c; p18-INK6;

免疫原

免疫原:
Uniprot:

P42773(CDN2C_HUMAN) >>Visit HPA database.

遺伝子(ID):
CDKN2C(1031)
発現特異性:
P42773 CDN2C_HUMAN:

Highest levels found in skeletal muscle. Also found in pancreas and heart.

タンパク質の説明:
Interacts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB.
タンパク質配列:
MAEPWGNELASAAARGDLEQLTSLLQNNVNVNAQNGFGRTALQVMKLGNPEIARRLLLRGANPDLKDRTGFAVIHDAARAGFLDTLQTLLEFQADVNIEDNEGNLPLHLAAKEGHLRVVEFLVKHTASNVGHRNHKGDTACDLARLYGRNEVVSLMQANGAGGATNLQ

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Bovine
91
Sheep
91
Dog
91
Horse
82
Rabbit
82
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P42773 基板として

Site PTM Type Enzyme
K46 Ubiquitination
K66 Ubiquitination
K136 Ubiquitination

研究背景

機能:

Interacts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB.

組織特異性:

Highest levels found in skeletal muscle. Also found in pancreas and heart.

サブユニット構造:

Heterodimer of p18 with CDK6.

タンパク質ファミリー:

Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.

研究領域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

参考文献

1). Oxidative modification of miR-30c promotes cardiac fibroblast proliferation via CDKN2C mismatch. Scientific reports, 2024 (PubMed: 38849466) [IF=4.6]

Application: WB    Species: Mouse    Sample:

Figure 5. CDKN2C is the target of 4,5-oxo-miR-30c. (A) 4,5-oxo-miR-30c and homologies of its predicted target sites in the 3′UTR of CDKN2C mRNA. (B) Luciferase reporter assays for the 3′UTR of CDKN2C mRNA using synthesized 4,5-oxo-miR-30c, 4,5-mut-miR-30c, and miR-30c. Activity is reported relative to the nontarget control. *P 

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