製品: Caveolin 1 Antibody
カタログ: AF5372
タンパク質の説明: Rabbit polyclonal antibody to Caveolin 1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 20 kDa; 20kD(Calculated).
ユニプロット: Q03135
RRID: AB_2837857

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(85%), Bovine(85%), Horse(100%), Sheep(85%), Rabbit(85%), Dog(85%)
クローナリティ:
Polyclonal
特異性:
Caveolin 1 Antibody detects endogenous levels of total Caveolin 1.
RRID:
AB_2837857
引用形式: Affinity Biosciences Cat# AF5372, RRID:AB_2837857.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

BSCL3; CAV; CAV1; CAV1_HUMAN; caveolae protein, 22 kD; caveolin 1 alpha isoform; caveolin 1 beta isoform; Caveolin 1 caveolae protein 22kDa; Caveolin-1; Caveolin1; cell growth-inhibiting protein 32; CGL3; LCCNS; MSTP085; OTTHUMP00000025031; PPH3; VIP 21; VIP21;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q03135 CAV1_HUMAN:

Skeletal muscle, liver, stomach, lung, kidney and heart (at protein level). Expressed in the brain.

タンパク質の説明:
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner
タンパク質配列:
MSGGKYVDSEGHLYTVPIREQGNIYKPNNKAMADELSEKQVYDAHTKEIDLVNRDPKHLNDDVVKIDFEDVIAEPEGTHSFDGIWKASFTTFTVTKYWFYRLLSALFGIPMALIWGIYFAILSFLHIWAVVPCIKSFLIEIQCISRVYSIYVHTVCDPLFEAVGKIFSNVRINLQKEI

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Pig
85
Bovine
85
Sheep
85
Dog
85
Rabbit
85
Zebrafish
62
Chicken
46
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q03135 基板として

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
K5 Acetylation
K5 Ubiquitination
Y6 Phosphorylation
S9 Phosphorylation
Y14 Phosphorylation P12931 (SRC) , P06241 (FYN) , P00519 (ABL1)
T15 Phosphorylation
Y25 Phosphorylation
K26 Sumoylation
K26 Ubiquitination
K30 Ubiquitination
S37 Phosphorylation
K39 Ubiquitination
Y42 Phosphorylation
T46 Phosphorylation
K47 Ubiquitination
K57 Acetylation
K57 Ubiquitination
K65 Ubiquitination
S80 Phosphorylation
S88 Phosphorylation
S168 Phosphorylation
K176 Ubiquitination

研究背景

機能:

May act as a scaffolding protein within caveolar membranes. Forms a stable heterooligomeric complex with CAV2 that targets to lipid rafts and drives caveolae formation. Mediates the recruitment of CAVIN proteins (CAVIN1/2/3/4) to the caveolae. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway (By similarity). Negatively regulates TGFB1-mediated activation of SMAD2/3 by mediating the internalization of TGFBR1 from membrane rafts leading to its subsequent degradation.

PTMs:

Ubiquitinated. Undergo monoubiquitination and multi- and/or polyubiquitination. Monoubiquitination of N-terminal lysines promotes integration in a ternary complex with UBXN6 and VCP which promotes oligomeric CAV1 targeting to lysosomes for degradation.

The initiator methionine for isoform 2 is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.

Phosphorylated at Tyr-14 by ABL1 in response to oxidative stress.

細胞の位置付け:

Golgi apparatus membrane>Peripheral membrane protein. Cell membrane>Peripheral membrane protein. Membrane>Caveola>Peripheral membrane protein. Membrane raft. Golgi apparatus>trans-Golgi network.
Note: Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Skeletal muscle, liver, stomach, lung, kidney and heart (at protein level). Expressed in the brain.

サブユニット構造:

Homooligomer. Interacts with GLIPR2. Interacts with NOSTRIN. Interacts with SNAP25 and STX1A (By similarity). Interacts (via the N-terminus) with DPP4; the interaction is direct. Interacts with CTNNB1, CDH1 and JUP. Interacts with PACSIN2; this interaction induces membrane tubulation (By similarity). Interacts with SLC7A9 (By similarity). Interacts with BMX and BTK. Interacts with TGFBR1. Interacts with CAVIN3 (via leucine-zipper domain) in a cholesterol-sensitive manner. Interacts with CAVIN1. Interacts with EHD2 in a cholesterol-dependent manner. Forms a ternary complex with UBXN6 and VCP; mediates CAV1 targeting to lysosomes for degradation. Interacts with ABCG1; this interaction regulates ABCG1-mediated cholesterol efflux.

(Microbial infection) Interacts with rotavirus A NSP4.

タンパク質ファミリー:

Belongs to the caveolin family.

研究領域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cardiovascular diseases > Viral myocarditis.

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