Caspase 10 Antibody - #AF0122

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製品: Caspase 10 Antibody
カタログ: AF0122
タンパク質の説明: Rabbit polyclonal antibody to Caspase 10
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse
予測: Horse, Rabbit, Xenopus
分子量: 60kDa; 59kD(Calculated).
ユニプロット: Q92851
RRID: AB_2833306

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Horse(100%), Rabbit(86%), Xenopus(86%)
クローナリティ:
Polyclonal
特異性:
Caspase 10 Antibody detects endogenous levels of total Caspase 10.
RRID:
AB_2833306
引用形式: Affinity Biosciences Cat# AF0122, RRID:AB_2833306.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ALPS2; Apoptotic protease Mch-4; CASP 10; CASP-10; CASP10; CASPA_HUMAN; Caspase 10 apoptosis related cysteine peptidase; Caspase-10 subunit p12; FADD like ICE2; Fas associated death domain protein; FAS-associated death domain protein interleukin-1B-converting enzyme 2; FLICE 2; FLICE2; ICE like apoptotic protease 4; ICE-like apoptotic protease 4; Interleukin 1B converting enzyme 2; MCH 4;

免疫原

免疫原:
Uniprot:

Q92851(CASPA_HUMAN) >>Visit HPA database.

遺伝子(ID):
CASP10(843)
発現特異性:
Q92851 CASPA_HUMAN:

Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.

タンパク質の説明:
Casp10 Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase- 3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC. Belongs to the peptidase C14A family. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 23/17 kDa (p23/17) (depending on the splicing events) and a 12 kDa (p12) subunit. Self-associates. Interacts with FADD and CASP8. Found in a Fas signaling complex consisting of FAS, FADD, CASP8 and CASP10. 6 isoforms of the human protein are produced by alternative splicing.
タンパク質配列:
MKSQGQHWYSSSDKNCKVSFREKLLIIDSNLGVQDVENLKFLCIGLVPNKKLEKSSSASDVFEHLLAEDLLSEEDPFFLAELLYIIRQKKLLQHLNCTKEEVERLLPTRQRVSLFRNLLYELSEGIDSENLKDMIFLLKDSLPKTEMTSLSFLAFLEKQGKIDEDNLTCLEDLCKTVVPKLLRNIEKYKREKAIQIVTPPVDKEAESYQGEEELVSQTDVKTFLEALPQESWQNKHAGSNGNRATNGAPSLVSRGMQGASANTLNSETSTKRAAVYRMNRNHRGLCVIVNNHSFTSLKDRQGTHKDAEILSHVFQWLGFTVHIHNNVTKVEMEMVLQKQKCNPAHADGDCFVFCILTHGRFGAVYSSDEALIPIREIMSHFTALQCPRLAEKPKLFFIQACQGEEIQPSVSIEADALNPEQAPTSLQDSIPAEADFLLGLATVPGYVSFRHVEEGSWYIQSLCNHLKKLVPRMLKFLEKTMEIRGRKRTVWGAKQISATSLPTAISAQTPRPPMRRWSSVS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Xenopus
86
Rabbit
86
Dog
73
Chicken
67
Pig
0
Bovine
0
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92851 基板として

Site PTM Type Enzyme
K2 Methylation
K2 Ubiquitination
S3 Phosphorylation
Y9 Phosphorylation
K14 Ubiquitination
K17 Ubiquitination
K23 Ubiquitination
K90 Ubiquitination
K99 Ubiquitination
K158 Ubiquitination
K161 Ubiquitination
K175 Ubiquitination
K180 Ubiquitination
K192 Ubiquitination
T198 Phosphorylation
S207 Phosphorylation
S216 Phosphorylation
K235 Ubiquitination
S250 Phosphorylation
S260 Phosphorylation
T263 Phosphorylation
T268 Phosphorylation
S269 Phosphorylation
K271 Ubiquitination
S293 Phosphorylation
K338 Ubiquitination

研究背景

機能:

Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC.

Isoform 7 can enhance NF-kappaB activity but promotes only slight apoptosis.

Isoform C is proteolytically inactive.

PTMs:

Cleavage by granzyme B and autocatalytic activity generate the two active subunits.

組織特異性:

Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.

サブユニット構造:

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 23/17 kDa (p23/17) (depending on the splicing events) and a 12 kDa (p12) subunit (By similarity). Self-associates. Interacts with FADD and CASP8. Found in a Fas signaling complex consisting of FAS, FADD, CASP8 and CASP10. Interacts with RFFL and RNF34; negatively regulate CASP10 through proteasomal degradation. Interacts with RIOK3.

タンパク質ファミリー:

Belongs to the peptidase C14A family.

研究領域

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.   (View pathway)

参考文献

1). MicroRNA-181a regulates epithelial-mesenchymal transition by targeting PTEN in drug-resistant lung adenocarcinoma cells. INTERNATIONAL JOURNAL OF ONCOLOGY, 2015 (PubMed: 26323677) [IF=5.2]

Application: WB    Species: Human    Sample: lung adenocarcinoma cells

Figure 3. A549/DDP and A549/PTX cells showed molecular and morphological changes that were consistent with EMT. (A) Microscopy at x200 magnification was used to assess cell morphology. The A549 cells (parental cells) had an epithelioid, rounded cobblestone appearance and there was limited formation of pseudopodia. A549/PTX and A549/DDP cells exhibited a spindle-shaped morphology and an increased formation of pseudopodia, indicating a loss of cell polarity. (B) E-cadherin, β -catenin, vimentin, MMP-2 and MMP-9 which are EMT-related proteins, were assessed in terms of expression levels. EMT-related transcription factors (Snail, Slug, Twist and ZEB1) were measured in A549/PTX and A549/DDP cells using western blot analysis. (C) The expression changes were confirmed at the mRNA level by qRT-PCR. Expression was standardized to the expression of GAPDH and normalized to 1.0 in the parental cells (compared with the parental A549 cells, means ± SEM, n=3, * P<0.05).

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