HNF1 beta Antibody - #AF5394

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製品: HNF1 beta Antibody
カタログ: AF5394
タンパク質の説明: Rabbit polyclonal antibody to HNF1 beta
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 45kDa, 61kDa; 61kD(Calculated).
ユニプロット: P35680
RRID: AB_2837879

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 100ul $280 在庫あり
 200ul $350 在庫あり

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関連ダウンロード
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
クローナリティ:
Polyclonal
特異性:
HNF1 beta Antibody detects endogenous levels of total HNF1 beta.
RRID:
AB_2837879
引用形式: Affinity Biosciences Cat# AF5394, RRID:AB_2837879.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

FJHN; Hepatocyte nuclear factor 1 beta; Hepatocyte nuclear factor 1-beta; HNF 1B; HNF 2; HNF-1-beta; HNF-1B; HNF1 beta; HNF1 homeobox B; HNF1B; HNF1B_HUMAN; HNF1beta; HNF2; Homeoprotein LF B3; Homeoprotein LFB3; HPC11; LF B3; LFB3; MODY 5; MODY5; TCF 2; TCF 2 protein; TCF-2; TCF2; TCF2 protein; Transcription factor 2; Transcription factor 2 hepatic; Variant hepatic nuclear factor 1; Variant hepatic nuclear factor; VHNF 1; vHNF1;

免疫原

免疫原:
Uniprot:

P35680(HNF1B_HUMAN) >>Visit HPA database.

遺伝子(ID):
HNF1B(6928)
タンパク質の説明:
Defects in HNF1B are the cause of renal cysts and diabetes syndrome (RCAD) [MIM:137920]; also called maturity-onset diabetes of the young type 5 (MODY5) or familial hypoplastic glomerulocystic kidney disease (GCKD). RCAD is an autosomal dominant disorder comprising non-diabetic renal disease resulting from abnormal renal development, and diabetes, which in some cases occurs earlier than age 25 years and is thus consistent with a diagnosis of maturity-onset diabetes of the young (MODY5).
タンパク質配列:
MVSKLTSLQQELLSALLSSGVTKEVLVQALEELLPSPNFGVKLETLPLSPGSGAEPDTKPVFHTLTNGHAKGRLSGDEGSEDGDDYDTPPILKELQALNTEEAAEQRAEVDRMLSEDPWRAAKMIKGYMQQHNIPQREVVDVTGLNQSHLSQHLNKGTPMKTQKRAALYTWYVRKQREILRQFNQTVQSSGNMTDKSSQDQLLFLFPEFSQQSHGPGQSDDACSEPTNKKMRRNRFKWGPASQQILYQAYDRQKNPSKEEREALVEECNRAECLQRGVSPSKAHGLGSNLVTEVRVYNWFANRRKEEAFRQKLAMDAYSSNQTHSLNPLLSHGSPHHQPSSSPPNKLSGVRYSQQGNNEITSSSTISHHGNSAMVTSQSVLQQVSPASLDPGHNLLSPDGKMISVSGGGLPPVSTLTNIHSLSHHNPQQSQNLIMTPLSGVMAIAQSLNTSQAQSVPVINSVAGSLAALQPVQFSQQLHSPHQQPLMQQSPGSHMAQQPFMAAVTQLQNSHMYAHKQEPPQYSHTSRFPSAMVVTDTSSISTLTNMSSSKQCPLQAW

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
92
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35680 基板として

Site PTM Type Enzyme
S49 Phosphorylation
S52 Phosphorylation
S75 Phosphorylation
S80 Phosphorylation
T88 Phosphorylation
S279 Phosphorylation
S281 Phosphorylation
S288 Phosphorylation
S363 Phosphorylation
S538 Phosphorylation

研究背景

機能:

Transcription factor, probably binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Binds DNA as a dimer. Can form homodimer or heterodimer with HNF1-alpha.

タンパク質ファミリー:

Belongs to the HNF1 homeobox family.

研究領域

· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.

参考文献

1). Acetaminophen-induced hepatocyte injury: C2-ceramide and oltipraz intervention, hepatocyte nuclear factor 1 and glutathione S-transferase A1 changes. JOURNAL OF APPLIED TOXICOLOGY, 2019 (PubMed: 31385618) [IF=3.3]

Application: WB    Species: Mice    Sample: hepatocytes

FIGURE 6 A, Original blots for HNF‐1, GSTA1 and β‐actin. B, HNF‐1 protein level. C, GSTA1 protein level. Changes in HNF‐1 and GSTA1 protein levels in hepatocytes after 15 mmol/L APAP exposure for 10 h with C2 (6 μmol/L) or OL (8 μmol/L). Values are expressed as the mean ± SD for each group (n = 4). *P < .05, **P < .01 represented all groups compared with the control group; # P < .05, ## P < .01 represented all groups compared with the APAP model group. APAP, acetaminophen; C2, C2‐ceramide; GSTA1, glutathione S‐transferase A1; HNF‐1, hepatocyte nuclear factor 1; OL, oltipraz

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