PGC1 Antibody - #AF5395
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF5395, RRID:AB_2837880.
折りたたみ/展開
L PGC 1alpha; LEM6; Ligand effect modulator 6; Peroxisome proliferative activated receptor gamma coactivator 1 alpha; Peroxisome proliferative activated receptor gamma coactivator 1; Peroxisome proliferator activated receptor gamma coactivator 1 alpha; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 3ext; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 8a; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 NT; Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; PGC 1 (alpha); PGC 1 alpha; PGC 1v; PGC-1-alpha; PGC1; PGC1(alpha); PGC1A; PGC1v; PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6; PPAR gamma coactivator 1 alpha; PPAR gamma coactivator 1; PPAR gamma coactivator variant form; PPAR-gamma coactivator 1-alpha; PPARGC 1 alpha; PPARGC-1-alpha; PPARGC1; PPARGC1A; PRGC1_HUMAN; PPARGC1B, PERC, PGC1, PGC1B, PPARGC1; Peroxisome proliferator-activated receptor gamma coactivator 1-beta;
免疫原
Heart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects.
Q86YN6 PRGC2_HUMAN:Ubiquitous with higher expression in heart, brain and skeletal muscle.
- Q9UBK2 PRGC1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAWDMCNQDSESVWSDIECAALVGEDQPLCPDLPELDLSELDVNDLDTDSFLGGLKWCSDQSEIISNQYNNEPSNIFEKIDEENEANLLAVLTETLDSLPVDEDGLPSFDALTDGDVTTDNEASPSSMPDGTPPPQEAEEPSLLKKLLLAPANTQLSYNECSGLSTQNHANHNHRIRTNPAIVKTENSWSNKAKSICQQQKPQRRPCSELLKYLTTNDDPPHTKPTENRNSSRDKCTSKKKSHTQSQSQHLQAKPTTLSLPLTPESPNDPKGSPFENKTIERTLSVELSGTAGLTPPTTPPHKANQDNPFRASPKLKSSCKTVVPPPSKKPRYSESSGTQGNNSTKKGPEQSELYAQLSKSSVLTGGHEERKTKRPSLRLFGDHDYCQSINSKTEILINISQELQDSRQLENKDVSSDWQGQICSSTDSDQCYLRETLEASKQVSPCSTRKQLQDQEIRAELNKHFGHPSQAVFDDEADKTGELRDSDFSNEQFSKLPMFINSGLAMDGLFDDSEDESDKLSYPWDGTQSYSLFNVSPSCSSFNSPCRDSVSPPKSLFSQRPQRMRSRSRSFSRHRSCSRSPYSRSRSRSPGSRSSSRSCYYYESSHYRHRTHRNSPLYVRSRSRSPYSRRPRYDSYEEYQHERLKREEYRREYEKRESERAKQRERQRQKAIEERRVIYVGKIRPDTTRTELRDRFEVFGEIEECTVNLRDDGDSYGFITYRYTCDAFAALENGYTLRRSNETDFELYFCGRKQFFKSNYADLDSNSDDFDPASTKSKYDSLDFDSLLKEAQRSLRR
- Q86YN6 PRGC2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGNDCGALLDEELSSFFLNYLADTQGGGSGEEQLYADFPELDLSQLDASDFDSATCFGELQWCPENSETEPNQYSPDDSELFQIDSENEALLAELTKTLDDIPEDDVGLAAFPALDGGDALSCTSASPAPSSAPPSPAPEKPSAPAPEVDELSLLQKLLLATSYPTSSSDTQKEGTAWRQAGLRSKSQRPCVKADSTQDKKAPMMQSQSRSCTELHKHLTSAQCCLQDRGLQPPCLQSPRLPAKEDKEPGEDCPSPQPAPASPRDSLALGRADPGAPVSQEDMQAMVQLIRYMHTYCLPQRKLPPQTPEPLPKACSNPSQQVRSRPWSRHHSKASWAEFSILRELLAQDVLCDVSKPYRLATPVYASLTPRSRPRPPKDSQASPGRPSSVEEVRIAASPKSTGPRPSLRPLRLEVKREVRRPARLQQQEEEDEEEEEEEEEEEKEEEEEWGRKRPGRGLPWTKLGRKLESSVCPVRRSRRLNPELGPWLTFADEPLVPSEPQGALPSLCLAPKAYDVERELGSPTDEDSGQDQQLLRGPQIPALESPCESGCGDMDEDPSCPQLPPRDSPRCLMLALSQSDPTFGKKSFEQTLTVELCGTAGLTPPTTPPYKPTEEDPFKPDIKHSLGKEIALSLPSPEGLSLKATPGAAHKLPKKHPERSELLSHLRHATAQPASQAGQKRPFSCSFGDHDYCQVLRPEGVLQRKVLRSWEPSGVHLEDWPQQGAPWAEAQAPGREEDRSCDAGAPPKDSTLLRDHEIRASLTKHFGLLETALEEEDLASCKSPEYDTVFEDSSSSSGESSFLPEEEEEEGEEEEEDDEEEDSGVSPTCSDHCPYQSPPSKANRQLCSRSRSSSGSSPCHSWSPATRRNFRCESRGPCSDRTPSIRHARKRREKAIGEGRVVYIQNLSSDMSSRELKRRFEVFGEIEECEVLTRNRRGEKYGFITYRCSEHAALSLTKGAALRKRNEPSFQLSYGGLRHFCWPRYTDYDSNSEEALPASGKSKYEAMDFDSLLKEAQQSLH
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UBK2,Q86YN6 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
T178 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
S208 | Phosphorylation | Uniprot | |
S246 | Phosphorylation | Uniprot | |
T263 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
S266 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
S273 | Phosphorylation | Uniprot | |
K278 | Acetylation | Uniprot | |
S285 | Phosphorylation | Uniprot | |
T295 | Phosphorylation | Uniprot | |
T299 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
S313 | Phosphorylation | Uniprot | |
K347 | Acetylation | Uniprot | |
K360 | Acetylation | Uniprot | |
K360 | Ubiquitination | Uniprot | |
S416 | Phosphorylation | Uniprot | |
S417 | Phosphorylation | Uniprot | |
K442 | Ubiquitination | Uniprot | |
K464 | Ubiquitination | Uniprot | |
K480 | Ubiquitination | Uniprot | |
S539 | Phosphorylation | Uniprot | |
S571 | Phosphorylation | P31751 (AKT2) , P31749 (AKT1) | Uniprot |
S616 | Phosphorylation | Uniprot | |
Y634 | Phosphorylation | Uniprot | |
S636 | Phosphorylation | Uniprot | |
Y717 | Phosphorylation | Uniprot | |
T721 | Phosphorylation | Uniprot | |
Y722 | Phosphorylation | Uniprot | |
K758 | Ubiquitination | Uniprot | |
S768 | Phosphorylation | Uniprot | |
K777 | Ubiquitination | Uniprot | |
K779 | Ubiquitination | Uniprot | |
S782 | Phosphorylation | Uniprot | |
S787 | Phosphorylation | Uniprot | |
K790 | Ubiquitination | Uniprot |
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S239 | Phosphorylation | Uniprot | |
S256 | Phosphorylation | Uniprot | |
S263 | Phosphorylation | Uniprot | |
K303 | Ubiquitination | Uniprot | |
K314 | Ubiquitination | Uniprot | |
K357 | Ubiquitination | Uniprot | |
S384 | Phosphorylation | Uniprot | |
S524 | Phosphorylation | Uniprot | |
S570 | Phosphorylation | P31749 (AKT1) | Uniprot |
S638 | Phosphorylation | Uniprot | |
S643 | Phosphorylation | Uniprot | |
T647 | Phosphorylation | Uniprot | |
S711 | Phosphorylation | Uniprot | |
S854 | Phosphorylation | Uniprot | |
S856 | Phosphorylation | Uniprot | |
S859 | Phosphorylation | Uniprot | |
S865 | Phosphorylation | Uniprot | |
Y943 | Phosphorylation | Uniprot | |
T947 | Phosphorylation | Uniprot | |
Y948 | Phosphorylation | Uniprot | |
Y987 | Phosphorylation | Uniprot | |
Y990 | Phosphorylation | Uniprot | |
S992 | Phosphorylation | Uniprot |
研究背景
Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK.
Phosphorylation by AMPK in skeletal muscle increases activation of its own promoter. Phosphorylated by CLK2.
Heavily acetylated by GCN5 and biologically inactive under conditions of high nutrients. Deacetylated by SIRT1 in low nutrients/high NAD conditions.
Ubiquitinated. Ubiquitination by RNF34 induces proteasomal degradation.
Nucleus. Nucleus>PML body.
Nucleus.
Cytoplasm. Nucleus.
Nucleus. Nucleus>PML body.
Nucleus.
Heart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects.
Homooligomer. Interacts with MYBBP1A; inhibits MYBBP1A transcriptional activation (By similarity). Interacts with PRDM16, LPIN1 and PML (By similarity). Interacts (via LXXLL motif) with RORA and RORC (via AF-2 motif); activates RORA and RORC transcriptional activation (By similarity). Interacts with LRPPRC. Interacts with RNF34 (via RING-type zinc finger).
Plays a role of stimulator of transcription factors and nuclear receptors activities. Activates transcriptional activity of estrogen receptor alpha, nuclear respiratory factor 1 (NRF1) and glucocorticoid receptor in the presence of glucocorticoids. May play a role in constitutive non-adrenergic-mediated mitochondrial biogenesis as suggested by increased basal oxygen consumption and mitochondrial number when overexpressed. May be involved in fat oxidation and non-oxidative glucose metabolism and in the regulation of energy expenditure. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner.
Nucleus.
Ubiquitous with higher expression in heart, brain and skeletal muscle.
Interacts with hepatocyte nuclear factor 4-alpha/HNF4A, Sterol regulatory binding transcription factor 1/SREBF1, PPAR-alpha/PPARA, thyroid hormone receptor beta/THRB and host cell factor/HCFC1. Interacts with estrogen-related receptor gamma/ESRRG and alpha/ESRRA. Interacts with PRDM16 (By similarity). Interacts with estrogen receptor alpha/ESR1.
Contains 2 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motif, which are usually required for the association with nuclear receptors.
研究領域
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)
· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.
· Organismal Systems > Endocrine system > Glucagon signaling pathway.
参考文献
Application: WB Species: Mouse Sample:
Application: WB Species: Human Sample: L02 cell
Application: WB Species: Human Sample: HepG-2 cells
Application: IF/ICC Species: Human Sample: HepG-2 cells
Application: WB Species: Goat Sample: RECs
Application: WB Species: piglets Sample:
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