ATF4 Antibody | Affinity Biosciences

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FIGURE 4| EndoA2 modulated the MAPK signaling pathway in response to ERS.

Figure 1.

Figure 3.

Figure 4 POU4F3 activated the ERS pathways in LUAD cells according to WB.

製品:	ATF4 Antibody
カタログ:	AF5416
タンパク質の説明:	Rabbit polyclonal antibody to ATF4
アプリケーション:	WB
反応性:	Human, Mouse
予測:	Pig, Bovine, Dog
分子量:	39~49kD; 39kD(Calculated).
ユニプロット:	P18848
RRID:	AB_2837900

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サイズ	価格	在庫状況
100ul	$280	在庫あり
200ul	$350	在庫あり

プロトコル

製品説明

ソース:

Rabbit

アプリケーション:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:

Human,Mouse

予測:

Pig(86%), Bovine(86%), Dog(86%)

クローナリティ:

Polyclonal

特異性:

ATF4 Antibody detects endogenous levels of total ATF4.

RRID:

AB_2837900
引用形式: Affinity Biosciences Cat# AF5416, RRID:AB_2837900.

コンジュゲート:

Unconjugated.

精製:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

別名:

折りたたみ／展開

Activating transcription factor 4; ATF 4; ATF4; ATF4 protein; ATF4_HUMAN; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB 2; CREB-2; CREB2; Cyclic AMP dependent transcription factor ATF 4; Cyclic AMP response element binding protein 2; Cyclic AMP-dependent transcription factor ATF-4; Cyclic AMP-responsive element-binding protein 2; DNA binding protein TAXREB67; DNA-binding protein TAXREB67; Tax Responsive Enhancer Element B67; Tax-responsive enhancer element-binding protein 67; TaxREB67; TXREB;

免疫原

免疫原:

Uniprot:

P18848(ATF4_HUMAN) >>Visit HPA database.

遺伝子(ID):

ATF4(468)

タンパク質の説明:

タンパク質配列:

P18848 ATF4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTEMSFLSSEVLVGDLMSPFDQSGLGAEESLGLLDDYLEVAKHFKPHGFSSDKAKAGSSEWLAVDGLVSPSNNSKEDAFSGTDWMLEKMDLKEFDLDALLGIDDLETMPDDLLTTLDDTCDLFAPLVQETNKQPPQTVNPIGHLPESLTKPDQVAPFTFLQPLPLSPGVLSSTPDHSFSLELGSEVDITEGDRKPDYTAYVAMIPQCIKEEDTPSDNDSGICMSPESYLGSPQHSPSTRGSPNRSLPSPGVLCGSARPKPYDPPGEKMVAAKVKGEKLDKKLKKMEQNKTAATRYRQKKRAEQEALTGECKELEKKNEALKERADSLAKEIQYLKDLIEEVRKARGKKRVP

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Bovine

Dog

Horse

Sheep

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P18848 基板として

P18848

Site	PTM Type	Enzyme	Source
K45	Sumoylation		Uniprot
K45	Ubiquitination		Uniprot
K53	Sumoylation		Uniprot
K53	Ubiquitination		Uniprot
K55	Ubiquitination		Uniprot
S69	Phosphorylation		Uniprot
K75	Ubiquitination		Uniprot
K88	Ubiquitination		Uniprot
T107	Phosphorylation	P07949 (RET)	Uniprot
T114	Phosphorylation	P07949 (RET)	Uniprot
T115	Phosphorylation	P07949 (RET)	Uniprot
T119	Phosphorylation	P07949 (RET)	Uniprot
S215	Phosphorylation		Uniprot
S219	Phosphorylation		Uniprot
S224	Phosphorylation		Uniprot
S245	Phosphorylation	P51812 (RPS6KA3)	Uniprot
S248	Phosphorylation		Uniprot
K259	Ubiquitination		Uniprot
K267	Sumoylation		Uniprot
K267	Ubiquitination		Uniprot
K277	Ubiquitination		Uniprot
T293	Phosphorylation		Uniprot
Y295	Phosphorylation		Uniprot
K299	Ubiquitination		Uniprot
K311	Acetylation	PR:000007102 (EP300)	Uniprot
K329	Ubiquitination		Uniprot
K335	Ubiquitination		Uniprot
K343	Acetylation		Uniprot
K348	Acetylation		Uniprot

研究背景

P18848_ATF4_HUMAN

機能:

Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production (By similarity). It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I. Regulates the induction of DDIT3/CHOP and asparagine synthetase (ASNS) in response to endoplasmic reticulum (ER) stress. In concert with DDIT3/CHOP, activates the transcription of TRIB3 and promotes ER stress-induced neuronal apoptosis by regulating the transcriptional induction of BBC3/PUMA. Activates transcription of SIRT4. Regulates the circadian expression of the core clock component PER2 and the serotonin transporter SLC6A4. Binds in a circadian time-dependent manner to the cAMP response elements (CRE) in the SLC6A4 and PER2 promoters and periodically activates the transcription of these genes. During ER stress response, activates the transcription of NLRP1, possibly in concert with other factors.

PTMs:

Ubiquitinated by SCF(BTRC) in response to mTORC1 signal, followed by proteasomal degradation and leading to down-regulate expression of SIRT4.

Phosphorylated by NEK6 (By similarity). Phosphorylated on the betaTrCP degron motif at Ser-219, followed by phosphorylation at Thr-213, Ser-224, Ser-231, Ser-235 and Ser-248, promoting interaction with BTRC and ubiquitination. Phosphorylation is promoted by mTORC1 (By similarity).

Phosphorylated by NEK6.

細胞の位置付け:

Cytoplasm. Cell membrane. Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Colocalizes with GABBR1 in hippocampal neuron dendritic membranes (By similarity). Colocalizes with NEK6 at the centrosome (PubMed:20873783).

サブユニット構造:

Binds DNA as a homodimer and as a heterodimer. Interacts with CEBPB and binds DNA as a heterodimer with CEBPB. Interacts (via its leucine zipper domain) with GABBR1 and GABBR2 (via their C-termini). Interacts (via its DNA binding domain) with FOXO1 (C-terminal half); the interaction occurs in osteoblasts and regulates glucose homeostasis through suppression of beta-cell proliferation and a decrease in insulin production. Interacts with SATB2; the interaction results in enhanced DNA binding and transactivation by these transcription factors (By similarity). Interacts with CEP290 (via an N-terminal region). Interacts with NEK6, DAPK2 (isoform 2) and ZIPK/DAPK3. Forms a heterodimer with TXLNG in osteoblasts. Interacts with DDIT3/CHOP. Interacts with ABRAXAS2.

タンパク質ファミリー:

The BetaTrCP degron motif promotes binding to BTRC when phosphorylated.

Belongs to the bZIP family.

研究領域

· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Substance dependence > Cocaine addiction.

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Endocrine system > Insulin secretion. (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

参考文献

1). EndophilinA2 protects against angiotensin II-induced cardiac hypertrophy by inhibiting angiotensin II type 1 receptor trafficking in neonatal rat cardiomyocytes. JOURNAL OF CELLULAR BIOCHEMISTRY, 2018 (PubMed: 29923351) [IF=4.0]

Application: WB Species: rat Sample: cardiomyocytes

FIGURE 4| EndoA2 modulated the MAPK signaling pathway in response to ERS. A-F, Western blotting results show the expression levels of GRP78, p-PERK, p-eiF2α, ATF4, and CHOP after EndoA2 overexpression. Densitometric analyses show that EndoA2 overexpression decreased the expression levels of GRP78, p-PERK, p-eiF2α, ATF4, and CHOP, compared with those in the Ang II group. Transfection with AdlacZ had no significant effects (n = 5, *P < 0.05 vs control, #P < 0.05 vs Ang II).

2). POU4F3 Acts as a Tumor Suppressor in Lung Adenocarcinoma via the Endoplasmic Reticulum Stress Signaling Pathway. Journal of Cancer, 2022 (PubMed: 35069902) [IF=3.9]

Application: WB Species: Human Sample: LUAD cells

Figure 4 POU4F3 activated the ERS pathways in LUAD cells according to WB. A. The protein levels of PERK, p-PERK, eIF2α, p-eIF2α, ATF4, and CHOP in SPCA1 and A549 cells with or without POU4F3 overexpression, and with or without POU4F3 knockdown, were detected by WB. B. The protein levels of IRElα, p-IRElα, and XBP-1s in SPCA1 and A549 cells with or without POU4F3 overexpression, and with or without POU4F3 knockdown, were detected by WB.' ****', '***', and '**' represent P < 0.0001, 0.001, and 0.01 respectively.

3). Inhibition of the SIRT1 signaling pathway exacerbates endoplasmic reticulum stress induced by renal ischemia/reperfusion injury in type 1 diabetic rats. Molecular Medicine Reports, 2020 (PubMed: 31974604) [IF=3.4]

Application: WB Species: rat Sample: renal

Figure 3. |DM enhances endoplasmic reticulum stress. Western blot analysis was performed after 24 h of reperfusion. (A) Representative blots and histograms showing (B) p‑PERK/PERK ratio; (C) p‑eIF2α/eIF2α ratio; (D) ATF4 expression; and (E) CHOP expression.

4). Antitumor effect of ginsenoside Rg3 on gallbladder cancer by inducing endoplasmic reticulum stress-mediated apoptosis in vitro and in vivo. Oncology Letters, 2018 (PubMed: 30344724) [IF=2.9]

Application: WB Species: human Sample: GBC‑SD cells

Figure 1. |Rg3 activates ER stress in GBC‑SD cells. (A) GBC‑SD cells were treated with 1, 25, 50 and 100 µM Rg3 for 72 h, and proliferation was determined using a CCK‑8 kit. (B) Rg3 induced p‑eIF2α, ATF4 and Lcn2 expression in GBC‑SD cells. Relative protein expression level of (C) eIF2α, (D) p‑eIF2α, (E) ATF4 and (F) Lcn2 compared with the DMSO group. Relative protein expression was quantified by normalizing to the internal control β‑actin (n=3). *P<0.05, **P<0.01 vs. DMSO group. OD, optical density; ER, endoplasmic reticulum; eIF2α, eukaryotic translation‑initiation factor 2α; ATF4, activating transcription factor 4; p‑, phospho‑; DMSO, dimethylsulfoxide; Lcn2, lipocalin 2.

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ATF4 Antibody - #AF5416

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