Phospho-Cyclin H (Thr315) Antibody - #AF5426
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF5426, RRID:AB_2837910.
折りたたみ／展開
6330408H09Rik; AI661354; AV102684; AW538719; CAK; CAK complex subunit; ccnh; CCNH_HUMAN; CDK activating kinase; CDK activating kinase complex subunit; Cyclin dependent kinase activating kinase; cyclin dependent kinase activating kinase complex subunit; Cyclin H; Cyclin-H; CyclinH; MO15 associated protein; MO15-associated protein; p34; p36; p37;
免疫原
- P51946 CCNH_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MYHNSSQKRHWTFSSEEQLARLRADANRKFRCKAVANGKVLPNDPVFLEPHEEMTLCKYYEKRLLEFCSVFKPAMPRSVVGTACMYFKRFYLNNSVMEYHPRIIMLTCAFLACKVDEFNVSSPQFVGNLRESPLGQEKALEQILEYELLLIQQLNFHLIVHNPYRPFEGFLIDLKTRYPILENPEILRKTADDFLNRIALTDAYLLYTPSQIALTAILSSASRAGITMESYLSESLMLKENRTCLSQLLDIMKSMRNLVKKYEPPRSEEVAVLKQKLERCHSAELALNVITKKRKGYEDDDYVSKKSKHEEEEWTDDDLVESL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P51946 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S5 | Phosphorylation | P49336 (CDK8) | Uniprot |
| K8 | Ubiquitination | Uniprot | |
| K39 | Ubiquitination | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| K72 | Ubiquitination | Uniprot | |
| S132 | Phosphorylation | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| T227 | Phosphorylation | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| Y262 | Phosphorylation | Uniprot | |
| K274 | Ubiquitination | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| Y297 | Phosphorylation | Uniprot | |
| Y302 | Phosphorylation | Uniprot | |
| S304 | Phosphorylation | P49336 (CDK8) | Uniprot |
| S307 | Phosphorylation | Uniprot | |
| T315 | Phosphorylation | P68400 (CSNK2A1) , P19784 (CSNK2A2) | Uniprot |
| S322 | Phosphorylation | Uniprot |
研究背景
Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
Nucleus.
Associates primarily with CDK7 and MAT1 to form the CAK complex. CAK can further associate with the core-TFIIH to form the TFIIH basal transcription factor.
Belongs to the cyclin family. Cyclin C subfamily.
研究領域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Genetic Information Processing > Transcription > Basal transcription factors.
· Genetic Information Processing > Replication and repair > Nucleotide excision repair.
Restrictive clause
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For Research Use Only.
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