Phospho-Cyclin C (Ser275) Antibody - #AF5435

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製品: Phospho-Cyclin C (Ser275) Antibody
カタログ: AF5435
タンパク質の説明: Rabbit polyclonal antibody to Phospho-Cyclin C (Ser275)
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
分子量: 33 kDa; 33kD(Calculated).
ユニプロット: P24863
RRID: AB_2837919

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(80%)
クローナリティ:
Polyclonal
特異性:
Phospho-Cyclin C (Ser275) Antibody detects endogenous levels of Cyclin C only when phosphorylated at Ser275.
RRID:
AB_2837919
引用形式: Affinity Biosciences Cat# AF5435, RRID:AB_2837919.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ccnc; CCNC_HUMAN; CycC; Cyclin C; Cyclin-C; hSRB11; OTTHUMP00000016897; SRB11 homolog;

免疫原

免疫原:
Uniprot:

P24863(CCNC_HUMAN) >>Visit HPA database.

遺伝子(ID):
CCNC(892)
発現特異性:
P24863 CCNC_HUMAN:

Highest levels in pancreas. High levels in heart, liver, skeletal muscle and kidney. Low levels in brain.

タンパク質の説明:
Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery.
タンパク質配列:
MAGNFWQSSHYLQWILDKQDLLKERQKDLKFLSEEEYWKLQIFFTNVIQALGEHLKLRQQVIATATVYFKRFYARYSLKSIDPVLMAPTCVFLASKVEEFGVVSNTRLIAAATSVLKTRFSYAFPKEFPYRMNHILECEFYLLELMDCCLIVYHPYRPLLQYVQDMGQEDMLLPLAWRIVNDTYRTDLCLLYPPFMIALACLHVACVVQQKDARQWFAELSVDMEKILEIIRVILKLYEQWKNFDERKEMATILSKMPKPKPPPNSEGEQGPNGSQNSSYSQS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Chicken
100
Rabbit
100
Xenopus
80
Pig
0
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P24863 基板として

Site PTM Type Enzyme
K30 Ubiquitination
S33 Phosphorylation
Y73 Phosphorylation
S104 Phosphorylation
K117 Ubiquitination
S121 Phosphorylation
Y122 Phosphorylation
K126 Ubiquitination
K248 Ubiquitination
T252 Phosphorylation
S275 Phosphorylation
S278 Phosphorylation
S279 Phosphorylation
Y280 Phosphorylation
S281 Phosphorylation
S283 Phosphorylation

研究背景

機能:

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highest levels in pancreas. High levels in heart, liver, skeletal muscle and kidney. Low levels in brain.

サブユニット構造:

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. The cylin/CDK pair formed by CCNC/CDK8 also associates with the large subunit of RNA polymerase II.

タンパク質ファミリー:

Belongs to the cyclin family. Cyclin C subfamily.

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