Phospho-Caspase 1 (Ser376) Antibody - #AF5438
| 製品: | Phospho-Caspase 1 (Ser376) Antibody |
| カタログ: | AF5438 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-Caspase 1 (Ser376) |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Rabbit |
| 分子量: | 45 kDa; 45kD(Calculated). |
| ユニプロット: | P29466 |
| RRID: | AB_2837922 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF5438, RRID:AB_2837922.
折りたたみ／展開
CASP-1; CASP1; CASP1_HUMAN; Caspase 1; Caspase-1 subunit p10; ICE; IL-1 beta-converting enzyme; IL-1BC; IL1 beta converting enzyme; IL1B convertase; Interleukin 1 beta convertase; Interleukin 1B converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45;
免疫原
Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
- P29466 CASP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADKVLKEKRKLFIRSMGEGTINGLLDELLQTRVLNKEEMEKVKRENATVMDKTRALIDSVIPKGAQACQICITYICEEDSYLAGTLGLSADQTSGNYLNMQDSQGVLSSFPAPQAVQDNPAMPTSSGSEGNVKLCSLEEAQRIWKQKSAEIYPIMDKSSRTRLALIICNEEFDSIPRRTGAEVDITGMTMLLQNLGYSVDVKKNLTASDMTTELEAFAHRPEHKTSDSTFLVFMSHGIREGICGKKHSEQVPDILQLNAIFNMLNTKNCPSLKDKPKVIIIQACRGDSPGVVWFKDSVGVSGNLSLPTTEEFEDDAIKKAHIEKDFIAFCSSTPDNVSWRHPTMGSVFIGRLIEHMQEYACSCDVEEIFRKVRFSFEQPDGRAQMPTTERVTLTRCFYLFPGH
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P29466 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S16 | Phosphorylation | Uniprot | |
| T21 | Phosphorylation | Uniprot | |
| T32 | Phosphorylation | Uniprot | |
| K37 | Ubiquitination | Uniprot | |
| K44 | Ubiquitination | Uniprot | |
| T49 | Phosphorylation | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| K134 | Ubiquitination | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| T226 | Phosphorylation | Uniprot | |
| S227 | Phosphorylation | Uniprot | |
| K268 | Ubiquitination | Uniprot | |
| K274 | Ubiquitination | Uniprot | |
| K278 | Ubiquitination | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| K319 | Ubiquitination | Uniprot | |
| K320 | Ubiquitination | Uniprot | |
| K325 | Ubiquitination | Uniprot | |
| S376 | Phosphorylation | Uniprot |
研究背景
Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive. In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly.
The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
Cytoplasm. Cell membrane.
Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Both the p10 and p20 subunits interact with MEFV. Interacts with CARD17/INCA and CARD18. Interacts with SERPINB1; this interaction regulates CASP1 activity.
Belongs to the peptidase C14A family.
研究領域
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).
· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.
· Human Diseases > Infectious diseases: Bacterial > Pertussis.
· Human Diseases > Infectious diseases: Bacterial > Legionellosis.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway. (View pathway)
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