Phospho-MRLC1 (Ser19) Antibody - #AF5443
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| 製品: | Phospho-MRLC1 (Ser19) Antibody |
| カタログ: | AF5443 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-MRLC1 (Ser19) |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 20 kDa; 20kD(Calculated). |
| ユニプロット: | P24844 |
| RRID: | AB_2837927 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF5443, RRID:AB_2837927.
折りたたみ／展開
20 kDa myosin light chain;Human 20kDa myosin light chain (MLC2) mRNA complete cds;LC20;MGC3505;MLC 2;MLC-2C;MLC2;MLY 9;MRLC1;MYL9;MYL9_HUMAN;Myosin light chain 9 regulatory;Myosin light polypeptide 9 regulatory;myosin regulatory light chain 1;Myosin regulatory light chain 2;Myosin regulatory light chain 2 smooth muscle isoform;Myosin regulatory light chain 9;Myosin regulatory light chain MRLC1;Myosin regulatory light polypeptide 9;Myosin RLC;Myosin vascular smooth muscle light chain 2;MYRL2;OTTHUMP00000030857;smooth muscle isoform;
免疫原
- P24844 MYL9_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSKRAKAKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDHLRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDKDD
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P24844 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| S3 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| T10 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| T11 | Phosphorylation | Uniprot | |
| T19 | Phosphorylation | O43293 (DAPK3) , O14578 (CIT) , Q15746 (MYLK) , Q5VT25 (CDC42BPA) , Q13464 (ROCK1) | Uniprot |
| S20 | Phosphorylation | O43293 (DAPK3) , Q5VT25 (CDC42BPA) , O75116 (ROCK2) , Q14012 (CAMK1) , Q15746 (MYLK) , Q13153 (PAK1) , Q13177 (PAK2) , Q9Y5S2 (CDC42BPB) , O14578 (CIT) , Q13464 (ROCK1) | Uniprot |
| S29 | Phosphorylation | Uniprot | |
| K51 | Acetylation | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| S60 | Phosphorylation | Uniprot | |
| T97 | Phosphorylation | Uniprot | |
| S115 | Phosphorylation | Uniprot | |
| T128 | Phosphorylation | Uniprot | |
| T135 | Phosphorylation | Uniprot | |
| Y143 | Phosphorylation | P00533 (EGFR) | Uniprot |
| K150 | Ubiquitination | Uniprot | |
| K151 | Sumoylation | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| Y156 | Phosphorylation | P00533 (EGFR) | Uniprot |
| K164 | Ubiquitination | Uniprot | |
| K168 | Acetylation | Uniprot |
研究背景
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
Smooth muscle tissues and in some, but not all, nonmuscle cells.
Myosin is a hexamer of 2 heavy chains and 4 light chains: interacts with myosin heavy chain MYO19.
研究領域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
参考文献
Application: WB Species: rat Sample: hippocampus
Application: WB Species: rat Sample: RASMCs
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