COX IV Antibody - #AF5468

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製品: COX IV Antibody
カタログ: AF5468
タンパク質の説明: Rabbit polyclonal antibody to COX IV
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 17 kDa; 20kD(Calculated).
ユニプロット: P13073
RRID: AB_2837951

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MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(91%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(91%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
COX IV Antibody detects endogenous levels of total COX IV.
RRID:
AB_2837951
引用形式: Affinity Biosciences Cat# AF5468, RRID:AB_2837951.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

AL024441; COX 4; COX IV 1; COX IV; COX IV-1; Cox4; COX41_HUMAN; Cox4a; COX4B; COX4I1; COX4I2; COX4L2; COXIV; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit 4 isoform 1 mitochondrial; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; Cytochrome C Oxidase subunit IV; Cytochrome c oxidase subunit IV isoform 1; Cytochrome c oxidase subunit IV isoform 2 (lung); Cytochrome c oxydase subunit 4; dJ857M17.2; MGC105470; MGC72016;

免疫原

免疫原:

A synthesized peptide derived from human COX IV, corresponding to a region within the internal amino acids.

Uniprot:

P13073(COX41_HUMAN) >>Visit HPA database.

遺伝子(ID):
COX4I1(1327)
発現特異性:
P13073 COX41_HUMAN:

Ubiquitous.

タンパク質の説明:
This antibody makes an effective loading control for mitochondria. COX IV is generally expressed at a consistent high level. However, be aware that many proteins run at the same 16kD size as COX IV - our VDAC1 / Porin antibody makes a good alternative mitochondrial loading control for proteins of this size.
タンパク質配列:
MLATRVFSLVGKRAISTSVCVRAHESVVKSEDFSLPAYMDRRDHPLPEVAHVKHLSASQKALKEKEKASWSSLSMDEKVELYRIKFKESFAEMNRGSNEWKTVVGGAMFFIGFTALVIMWQKHYVYGPLPQSFDKEWVAKQTKRMLDMKVNPIQGLASKWDYEKNEWKK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Pig
91
Rabbit
91
Zebrafish
82
Xenopus
73
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunbit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

細胞の位置付け:

Mitochondrion inner membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitous.

タンパク質ファミリー:

Belongs to the cytochrome c oxidase IV family.

研究領域

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Circulatory system > Cardiac muscle contraction.   (View pathway)

参考文献

1). Zinc ions facilitate metabolic bioenergetic recovery post spinal cord injury by activating microglial mitophagy through the STAT3-FOXO3a-SOD2 pathway. Free radical biology & medicine, 2024 (PubMed: 39613048) [IF=7.1]
2). mTOR pathway mediates endoplasmic reticulum stress-induced CD4+ T cell apoptosis in septic mice. APOPTOSIS, 2022 (PubMed: 35759162) [IF=6.1]

Application: WB    Species: Mice    Sample: CD4+ T cells

Fig. 6 Levels of IRE1α-related pro-apoptotic protein caspase-3 and anti-apoptotic protein BCL-2. Expression of pro-apoptotic protein caspase-3 and anti-apoptotic protein BCL-2 (A, B) in CD4+ T cells. Protein expression were determined by western blotting and showed as the relative expression values of COX-IV. COX-IV was used as a loading control to normalize protein levels. Data are shown as Mean ± SD (n = 3). Statistically significant differences were determined by one-way analysis of variance (ANOVA) followed by Dunn’s/Tukey’s test. *P < 0.05, **P < 0.01, ****P < 0.0001
3). The Ca2+/CaMKK2 axis mediates the telbivudine induced upregulation of creatine kinase: Implications for mechanism of antiviral nucleoside analogs' side effect. BIOCHEMICAL PHARMACOLOGY, 2017 (PubMed: 29038020) [IF=5.3]

Application: WB    Species: human    Sample:

4). FGF21 attenuates pulmonary arterial hypertension via downregulation of miR‐130, which targets PPARγ. Journal of Cellular and Molecular Medicine, 2022 (PubMed: 34989130) [IF=5.3]

Application: WB    Species: Mice    Sample:

FIGURE 4 MiR‐130 inhibits hypoxia‐induced PASMC apoptosis by regulating PPARγ expression. (A–C) Western blotting for Bax, Bcl‐2, cleaved caspase‐3, caspase‐3 and apoptosis‐inducing factor (AIF) expression in PASMCs in Nor, Hyp, Hyp+miR‐130 inhibitor and Hyp+miR‐130 inhibitor+siPPARγ groups, β‐actin was used as a loading control (n = 4). (D‐F) The expression levels of cytochrome c (Cyt C) in mitochondrial and cytosol pellets in PASMCs were examined by western blotting with antibodies against Cyt C with COX IV as a mitochondria marker and β‐actin as the internal control (n = 4). (G) The apoptosis index of PASMCs in each group was measured by TUNEL assay (n = 10) (×200; scale bars indicate 100 µm) and is shown as the ratio of TUNEL positive cells (red) to total cells (blue). Data are presented as the mean ± SD. *p < 0.05, **p < 0.01
5). Baicalin alleviates bleomycin-induced early pulmonary fibrosis in mice via the mitoKATP signaling pathway. Toxicology, 2023 (PubMed: 37783230) [IF=4.8]
6). 2,3,5,4'-Tetrahydroxystilbene-2-O-beta-D-glucopyranoside promotes skin flap survival by promoting mitophagy through the PINK1/Parkin pathway. Journal of ethnopharmacology, 2025 (PubMed: 40043825) [IF=4.8]
7). Elucidation of SIRT-1/PGC-1α-associated mitochondrial dysfunction and autophagy in nonalcoholic fatty liver disease. Lipids in Health and Disease, 2021 (PubMed: 33902605) [IF=4.5]

Application: WB    Species: Mice    Sample: liver tissues

Fig. 5 Effect of intervene in SIRT-1 and combine with OA on p62, Beclin-1, LC3B, SIRT-1, PGC-1a, MFN1, MFF and COX-IV protein expression in HepG2 cells. HepG2 cells were treated with 1.5 mM OA for 48 h, following T6 (2 μM) or CAY (20 μM) 2 h. a The p62, Beclin-1 and LC3B protein expression was detected by Western blotting. b The same method was used to detect the SIRT-1, PGC-1a, MFN1, MFF and COX-IV protein expression. The bands were normalized using GAPDH. The images were quantified with ImageJ. Data are presented as the mean ± SD; *P < 0.05, **P < 0.01 compared with CON group; #P < 0.05, ##P < 0.01 compared with OA group
8). Cr (VI)-induced overactive mitophagy contributes to mitochondrial loss and cytotoxicity in L02 hepatocytes. Biochemical Journal, 2020 (PubMed: 32597464) [IF=4.4]
9). Hypoxia promotes the expression of Von Willebrand factor in breast cancer cells by up-regulating the transcription factor YY1 and down-regulating the hsa-miR-424. European Journal of Pharmacology, 2022 (PubMed: 36202224) [IF=4.2]
10). Equisetin inhibits adiposity through AMPK-dependent regulation of brown adipocyte differentiation. Heliyon, 2024 (PubMed: 38327434) [IF=4.0]
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