VDAC1 Antibody - #AF5478

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製品: VDAC1 Antibody
カタログ: AF5478
タンパク質の説明: Rabbit polyclonal antibody to VDAC1
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 31 kDa; 31kD(Calculated).
ユニプロット: P21796
RRID: AB_2837960

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説明書
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WB Handbook
IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
VDAC1 Antibody detects endogenous levels of total VDAC1.
RRID:
AB_2837960
引用形式: Affinity Biosciences Cat# AF5478, RRID:AB_2837960.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

N2441; OMP2; POR1; hVDAC1; MGC111064; Mitochondrial Porin; Outer mitochondrial membrane protein porin 1; Plasmalemmal porin; Porin 31HL; Porin 31HM; VDAC; VDAC-1; Vdac1; VDAC1_HUMAN; Voltage dependent anion channel 1; Voltage dependent anion selective channel protein 1; Voltage-dependent anion-selective channel protein 1; YNL055C; YNL2441C;

免疫原

免疫原:

A synthesized peptide derived from human VDAC1, corresponding to a region within C-terminal amino acids.

Uniprot:

P21796(VDAC1_HUMAN) >>Visit HPA database.

遺伝子(ID):
VDAC1(7416)
発現特異性:
P21796 VDAC1_HUMAN:

Heart, liver and skeletal muscle.

タンパク質の説明:
Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV.
タンパク質配列:
MAVPPTYADLGKSARDVFTKGYGFGLIKLDLKTKSENGLEFTSSGSANTETTKVTGSLETKYRWTEYGLTFTEKWNTDNTLGTEITVEDQLARGLKLTFDSSFSPNTGKKNAKIKTGYKREHINLGCDMDFDIAGPSIRGALVLGYEGWLAGYQMNFETAKSRVTQSNFAVGYKTDEFQLHTNVNDGTEFGGSIYQKVNKKLETAVNLAWTAGNSNTRFGIAAKYQIDPDACFSAKVNNSSLIGLGYTQTLKPGIKLTLSALLDGKNVNAGGHKLGLGLEFQA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.

PTMs:

Phosphorylation at Ser-193 by NEK1 promotes the open conformational state preventing excessive mitochondrial membrane permeability and subsequent apoptotic cell death after injury. Phosphorylation by the AKT-GSK3B axis stabilizes the protein probably by preventing ubiquitin-mediated proteasomal degradation.

Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.

細胞の位置付け:

Mitochondrion outer membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein. Membrane raft>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Heart, liver and skeletal muscle.

タンパク質ファミリー:

Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.

Belongs to the eukaryotic mitochondrial porin family.

研究領域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Digestive system > Cholesterol metabolism.

参考文献

1). The clinical antiprotozoal drug nitazoxanide and its metabolite tizoxanide extend Caenorhabditis elegans lifespan and healthspan. Acta pharmaceutica Sinica. B, 2024 (PubMed: 39027239) [IF=14.7]
2). VEGFR3 mitigates hypertensive nephropathy by enhancing mitophagy via regulating crotonylation of HSPA1L. Cell communication and signaling : CCS, 2025 (PubMed: 39875989) [IF=8.4]

Application: WB    Species: Mouse    Sample:

Fig. 2 Activation of VEGFR3 decreases renal oxidative stress levels and restored mitophagy in Ang II-induced hypertensive mice. A Significant statistics of GO term enrichment analysis (Control versus Ang II). B, C Renal tissues were stained to detect MitoSox Assay Kit (red). Scale bar, 100 μm. D Expressions of SQSTM1 and LC3 II/LC3 I were determined in mitochondria of the renal cortex from each group mice. E 3-nitrotyrosine, 8-oxo-dG, and SOD1 protein expression levels in renal cortex were examined by immunoblotting. F, G Immunoblots were statistically quantified by densitometry. H, I HE and Masson staining were carried out to assess the degree of renal tubular injury and fibrosis. J, K Semiquantitative scoring of tubular injury and renal fibrosis in different groups. Scale bar, 2–200 μm. n = 5 mice per group. Data in (B-K) were analyzed with one-way ANOVA. **P 
3). Piezo1 channel activation stimulates ATP production through enhancing mitochondrial respiration and glycolysis in vascular endothelial cells. British journal of pharmacology, 2023 (PubMed: 36740831) [IF=6.8]
4). Cyfluthrin exposure during pregnancy causes neurotoxicity in offspring-Ca2+ overload via IP3R-GRP75-VDAC1 pathway. Ecotoxicology and environmental safety, 2024 (PubMed: 38492481) [IF=6.2]
5). Activated PRDM1-CREBBP contributes to preeclampsia by regulating apoptosis and invasion of the human trophoblast cells. iScience, 2024 (PubMed: 39759022) [IF=5.8]
6). 3-MCPD Induced Mitochondrial Damage of Renal Cells Via the Rhythmic Protein BMAL1 Targeting SIRT3/SOD2. Journal of Agricultural and Food Chemistry, 2023 (PubMed: 37750480) [IF=5.7]
7). Methylseleninic Acid Elevating the Nrf2-GPX4 Axis Relieves Endothelial Dysfunction and Ferroptosis Induced by Arsenic Exposure. Journal of agricultural and food chemistry, 2025 (PubMed: 40071728) [IF=5.7]
8). Inhibition of METTL3 promotes mesangial cell mitophagy and attenuates glomerular damage by alleviating FOSL1 m6A modifications via IGF2BP2-dependent mechanisms. Biochemical pharmacology, 2025 (PubMed: 40081768) [IF=5.3]
9). α‑Phellandrene enhances the apoptosis of HT‑29 cells induced by 5‑fluorouracil by modulating the mitochondria‑dependent pathway. Oncology reports, 2024 (PubMed: 38456489) [IF=3.8]

Application: IF/ICC    Species: Human    Sample: HT-29 cells

Figure 3. Effect of α-PA combined with 5-FU treatment on VDAC-1 and HK-2 protein expression in HT-29 cells. HT-29 cells (1.0×105 cells/30-mm plate) were treated with 50, 100 or 250 µg/mM α-PA combined with 5-FU for 72 h. (A) Immunocytochemical staining was performed to determine the expression levels of HK-2 (green) and VDAC-1 (red) (Hoechst 33342 (blue) (×400 magnification). Quantitative analysis of the relative expression levels of (B) HK-2 and (C) VDAC-1 after treatment. Data are presented as the mean ± SD (n=3-5). *P
10). TRPV1 Inhibits Ferroptosis and Mitophagy to Alleviate Heart Failure by Activating SFXN2. Frontiers in bioscience (Landmark edition), 2025 (PubMed: 40613298) [IF=3.3]

Application: WB    Species: Mouse    Sample:

Fig. 5. TRPV1 overexpression regulates the expression of mitophagy-related proteins in the cytoplasm and mitochondria in the TAC-induced HF model. (A) Representative Western blot images showing the protein expression of mitophagy markers (Parkin, PTEN-induced kinase 1 (PINK1), and sequestosome 1 (SQSTM1)) in the cytoplasm and mitochondria fractions. GAPDH and voltage-dependent anion channel 1 (VDAC1) were used as loading controls for the cytoplasm and mitochondria fractions, respectively. (B) Quantification of the cytoplasmic protein levels of Parkin, PINK1, and SQSTM1 in the experimental groups, with GAPDH as the standard. (C) Quantification of the mitochondrial protein levels of Parkin, PINK1, and SQSTM1 in the experimental groups, with VDAC1 as the standard. n = 8 per group. Data are presented as mean ± standard deviation.
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