製品: Cytokeratin 5 Antibody
カタログ: AF5479
タンパク質の説明: Rabbit polyclonal antibody to Cytokeratin 5
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Rabbit
分子量: 62 kDa; 62kD(Calculated).
ユニプロット: P13647
RRID: AB_2837961

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Horse(100%), Rabbit(93%)
クローナリティ:
Polyclonal
特異性:
Cytokeratin 5 Antibody detects endogenous levels of total Cytokeratin 5.
RRID:
AB_2837961
引用形式: Affinity Biosciences Cat# AF5479, RRID:AB_2837961.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

58 kDa cytokeratin; CK-5; CK5; Cytokeratin-5; Cytokeratin5; DDD; DDD1; EBS2; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; K2C5_HUMAN; K5; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); Keratin 5; Keratin; keratin complex 2, basic, gene 5; keratin, type II cytoskeletal 5; Keratin-5; Keratin5; KRT 5; Krt5; KRT5A; type II cytoskeletal 5; Type-II keratin Kb5;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質の説明:
Defects in KRT5 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.
タンパク質配列:
MSRQSSVSFRSGGSRSFSTASAITPSVSRTSFTSVSRSGGGGGGGFGRVSLAGACGVGGYGSRSLYNLGGSKRISISTSGGSFRNRFGAGAGGGYGFGGGAGSGFGFGGGAGGGFGLGGGAGFGGGFGGPGFPVCPPGGIQEVTVNQSLLTPLNLQIDPSIQRVRTEEREQIKTLNNKFASFIDKVRFLEQQNKVLDTKWTLLQEQGTKTVRQNLEPLFEQYINNLRRQLDSIVGERGRLDSELRNMQDLVEDFKNKYEDEINKRTTAENEFVMLKKDVDAAYMNKVELEAKVDALMDEINFMKMFFDAELSQMQTHVSDTSVVLSMDNNRNLDLDSIIAEVKAQYEEIANRSRTEAESWYQTKYEELQQTAGRHGDDLRNTKHEISEMNRMIQRLRAEIDNVKKQCANLQNAIADAEQRGELALKDARNKLAELEEALQKAKQDMARLLREYQELMNTKLALDVEIATYRKLLEGEECRLSGEGVGPVNISVVTSSVSSGYGSGSGYGGGLGGGLGGGLGGGLAGGSSGSYYSSSSGGVGLGGGLSVGGSGFSASSGRGLGVGFGSGGGSSSSVKFVSTTSSSRKSFKS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Rabbit
93
Pig
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P13647 基板として

Site PTM Type Enzyme
S2 Phosphorylation
S5 Phosphorylation
S8 Phosphorylation
S11 Phosphorylation
S14 Phosphorylation
S16 Phosphorylation
S18 Phosphorylation
T19 Phosphorylation
S21 Phosphorylation
T24 Phosphorylation
S26 Phosphorylation
S28 Phosphorylation
S31 Phosphorylation
S36 Phosphorylation
S38 Phosphorylation
S50 Phosphorylation
Y60 Phosphorylation
S62 Phosphorylation
S64 Phosphorylation
Y66 Phosphorylation
S71 Phosphorylation
K72 Acetylation
K72 Methylation
S75 Phosphorylation
S77 Phosphorylation
T78 Phosphorylation
S82 Phosphorylation
T151 Phosphorylation
K173 Ubiquitination
T174 Phosphorylation
K178 Acetylation
K178 Methylation
K178 Sumoylation
K178 Ubiquitination
S181 Phosphorylation
K185 Acetylation
K185 Ubiquitination
K199 Methylation
Y222 Phosphorylation
S242 Phosphorylation
K257 Methylation
K257 Ubiquitination
Y258 Phosphorylation
K264 Methylation
K264 Ubiquitination
Y283 Phosphorylation
S337 Phosphorylation
Y346 Phosphorylation
S353 Phosphorylation
T355 Phosphorylation
S359 Phosphorylation
Y361 Phosphorylation
T363 Phosphorylation
Y365 Phosphorylation
K426 Ubiquitination
K443 Sumoylation
Y453 Phosphorylation
R480 Methylation
S567 Phosphorylation
S571 Phosphorylation
S574 Phosphorylation
S579 Phosphorylation
S587 Phosphorylation
S590 Phosphorylation

研究背景

サブユニット構造:

Heterodimer of a type I and a type II keratin. Heterodimer with type I keratin KRT25 leading to the formation of keratin intermediate filament (KIF) network. Heterodimer with KRT14. Interacts with TCHP. Interacts with EPPK1 (By similarity).

タンパク質ファミリー:

Belongs to the intermediate filament family.

参考文献

1). Network pharmacology combined with experimental validation reveals the mechanism of action of erpixing granules on functional dyspepsia. Journal of ethnopharmacology, 2024 (PubMed: 38992401) [IF=5.4]

2). Identification and functional study of immortalized mouse thymic epithelial cells. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2020 (PubMed: 32107001) [IF=3.1]

Application: IF/ICC    Species: mouse    Sample: thymic epithelial cells

Fig. 2. |(A) iTECs expressed the same epithelial cell-specific markers as TECs. DAPI (blue), CK5 (red) and CK8 (green) and Merge Images of the TECs Group and the iTECs Group;Photographs were made under a Microscope at a Magnification of 400

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