p63 Antibody - #AF5488
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製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF5488, RRID:AB_2837968.
折りたたみ／展開
AIS; Amplified in squamous cell carcinoma; B(p51A); B(p51B); Chronic ulcerative stomatitis protein; CUSP; DN p63 alpha 1; DNp63; EEC3; id:ibd3516; Keratinocyte transcription factor; Keratinocyte transcription factor KET; KET; LMS; MGC115972; MGC192897; NBP; OFC8; OTTHUMP00000209732; OTTHUMP00000209733; OTTHUMP00000209734; OTTHUMP00000209735; OTTHUMP00000209737; OTTHUMP00000209738; OTTHUMP00000209739; OTTHUMP00000209740; OTTHUMP00000209741; OTTHUMP00000209742; OTTHUMP00000209743; OTTHUMP00000209744; p40; p51; P51/P63; p53-related protein p63; p53CP; p63; P63_HUMAN; p73H; p73L; RHS; SHFM4; TAp63alpha; TP53CP; TP53L; TP63; TP73L; Transformation related protein 63; Transformation-related protein 63; Trp53rp1; Trp63; Tumor protein 63; Tumor protein p53-competing protein; Tumor protein p53-like; Tumor protein p63; Tumor protein p63 deltaN isoform delta; Tumor protein p73; Tumor protein p73-like;
免疫原
Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues.
- Q9H3D4 P63_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNFETSRCATLQYCPDPYIQRFVETPAHFSWKESYYRSTMSQSTQTNEFLSPEVFQHIWDFLEQPICSVQPIDLNFVDEPSEDGATNKIEISMDCIRMQDSDLSDPMWPQYTNLGLLNSMDQQIQNGSSSTSPYNTDHAQNSVTAPSPYAQPSSTFDALSPSPAIPSNTDYPGPHSFDVSFQQSSTAKSATWTYSTELKKLYCQIAKTCPIQIKVMTPPPQGAVIRAMPVYKKAEHVTEVVKRCPNHELSREFNEGQIAPPSHLIRVEGNSHAQYVEDPITGRQSVLVPYEPPQVGTEFTTVLYNFMCNSSCVGGMNRRPILIIVTLETRDGQVLGRRCFEARICACPGRDRKADEDSIRKQQVSDSTKNGDGTKRPFRQNTHGIQMTSIKKRRSPDDELLYLPVRGRETYEMLLKIKESLELMQYLPQHTIETYRQQQQQQHQHLLQKQTSIQSPSSYGNSSPPLNKMNSMNKLPSVSQLINPQQRNALTPTTIPDGMGANIPMMGTHMPMAGDMNGLSPTQALPPPLSMPSTSHCTPPPPYPTDCSIVSFLARLGCSSCLDYFTTQGLTTIYQIEHYSMDDLASLKIPEQFRHAIWKGILDHRQLHEFSSPSHLLRTPSSASTVSVGSSETRGERVIDAVRFTLRQTISFPPRDEWNDFNFDMDARRNKQQRIKEEGE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H3D4 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y13 | Phosphorylation | Uniprot | |
| Y18 | Phosphorylation | Uniprot | |
| Y36 | Phosphorylation | Uniprot | |
| S43 | Phosphorylation | O14920 (IKBKB) | Uniprot |
| T46 | Phosphorylation | Uniprot | |
| S51 | Phosphorylation | O14920 (IKBKB) | Uniprot |
| S68 | Phosphorylation | P36897 (TGFBR1) | Uniprot |
| Y149 | Phosphorylation | P00519 (ABL1) | Uniprot |
| S160 | Phosphorylation | P36897 (TGFBR1) | Uniprot |
| S162 | Phosphorylation | Uniprot | |
| Y171 | Phosphorylation | P00519 (ABL1) | Uniprot |
| Y231 | Phosphorylation | Uniprot | |
| K232 | Acetylation | Uniprot | |
| K242 | Methylation | Uniprot | |
| T281 | Phosphorylation | Uniprot | |
| Y290 | Phosphorylation | P00519 (ABL1) | Uniprot |
| T326 | Phosphorylation | Uniprot | |
| T329 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| S395 | Phosphorylation | Uniprot | |
| T451 | Phosphorylation | Uniprot | |
| S462 | Phosphorylation | Uniprot | |
| S463 | Phosphorylation | Uniprot | |
| S477 | Phosphorylation | P23443 (RPS6KB1) , Q13315 (ATM) , P24941 (CDK2) | Uniprot |
| S479 | Phosphorylation | Q13315 (ATM) | Uniprot |
| T491 | Phosphorylation | Q13315 (ATM) , P24941 (CDK2) , P23443 (RPS6KB1) , Q9H2X6 (HIPK2) | Uniprot |
| S560 | Phosphorylation | Q13315 (ATM) , P23443 (RPS6KB1) , P24941 (CDK2) | Uniprot |
| K588 | Sumoylation | Uniprot | |
| K599 | Ubiquitination | Uniprot | |
| K676 | Sumoylation | Uniprot |
研究背景
Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Activates transcription of the p21 promoter.
May be sumoylated.
Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.
Nucleus.
Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues.
Binds DNA as a homotetramer. Isoform composition of the tetramer may determine transactivation activity. Isoforms Alpha and Gamma interact with HIPK2. Interacts with SSRP1, leading to stimulate coactivator activity. Isoform 1 and isoform 2 interact with WWP1. Interacts with PDS5A. Isoform 5 (via activation domain) interacts with NOC2L.
The transactivation inhibitory domain (TID) can interact with, and inhibit the activity of the N-terminal transcriptional activation domain of TA*-type isoforms.
Belongs to the p53 family.
研究領域
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
参考文献
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