TSG6 Antibody - #AF5492

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製品: TSG6 Antibody
カタログ: AF5492
タンパク質の説明: Rabbit polyclonal antibody to TSG6
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 31 kDa; 31kD(Calculated).
ユニプロット: P98066
RRID: AB_2837972

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 100ul $280 在庫あり
 200ul $350 在庫あり

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関連ダウンロード
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(89%)
クローナリティ:
Polyclonal
特異性:
TSG6 Antibody detects endogenous levels of total TSG6.
RRID:
AB_2837972
引用形式: Affinity Biosciences Cat# AF5492, RRID:AB_2837972.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Hyaluronate binding protein; Hyaluronate-binding protein; TNF alpha induced protein 6; TNF alpha-induced protein 6; TNF stimulated gene 6 protein; TNF-stimulated gene 6 protein; TNFAIP 6; Tnfaip6; TSG 6; TSG-6; TSG6_HUMAN; Tumor necrosis factor alpha induced protein 6; Tumor necrosis factor alpha inducible protein 6; Tumor necrosis factor alpha-induced protein 6; Tumor necrosis factor inducible gene 6 protein; Tumor necrosis factor stimulated gene 6 protein; Tumor necrosis factor-inducible gene 6 protein;

免疫原

免疫原:
Uniprot:

P98066(TSG6_HUMAN) >>Visit HPA database.

遺伝子(ID):
TNFAIP6(7130)
発現特異性:
P98066 TSG6_HUMAN:

Found in the synovial fluid of patients with rheumatoid arthritis.

タンパク質の説明:
Possibly involved in cell-cell and cell-matrix interactions during inflammation and tumorigenesis.Subunit structure Interacts with inter-alpha-inhibitor (I-alpha-I). Chondroitin sulfate may be required for the stability of the complex.
タンパク質配列:
MIILIYLFLLLWEDTQGWGFKDGIFHNSIWLERAAGVYHREARSGKYKLTYAEAKAVCEFEGGHLATYKQLEAARKIGFHVCAAGWMAKGRVGYPIVKPGPNCGFGKTGIIDYGIRLNRSERWDAYCYNPHAKECGGVFTDPKQIFKSPGFPNEYEDNQICYWHIRLKYGQRIHLSFLDFDLEDDPGCLADYVEIYDSYDDVHGFVGRYCGDELPDDIISTGNVMTLKFLSDASVTAGGFQIKYVAMDPVSKSSQGKNTSTTSTGNKNFLAGRFSHL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Dog
89
Xenopus
78
Zebrafish
67
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P98066 基板として

Site PTM Type Enzyme
Y38 Phosphorylation
S148 Phosphorylation
Y155 Phosphorylation
T259 Phosphorylation
T262 Phosphorylation

研究背景

機能:

Possibly involved in cell-cell and cell-matrix interactions during inflammation and tumorigenesis.

組織特異性:

Found in the synovial fluid of patients with rheumatoid arthritis.

サブユニット構造:

Interacts with inter-alpha-inhibitor (I-alpha-I). Chondroitin sulfate may be required for the stability of the complex.

参考文献

1). A novel therapeutic approach for inflammatory bowel disease by exosomes derived from human umbilical cord mesenchymal stem cells to repair intestinal barrier via TSG-6. Stem Cell Research & Therapy, 2021 (PubMed: 34051868) [IF=7.5]

Application: WB    Species: Mice    Sample: colon tissue

Fig. 7 MSCs-Exo enhanced TSG-6 expression in the colitis mice colon. a TSG-6 expression levels in colon tissue in the control, DSS + PBS, and DSS + Exo groups 10 days after colitis induction. b TSG-6 expression in the DSS + siNC Exo and DSS + siTSG-6 Exo groups. c TSG-6 protein expression in MSC-Exo. d The levels of TSG-6 in MSC-Exo and supernatant without exosomes. Exosomes in the supernatant were removed by ultrafiltration. All samples were adjusted to the same protein concentration, followed by ELISA. Data are presented as mean ± SD; n = 4 per group. *P < 0.05, **P < 0.01 and ns indicates P > 0.05
2). Identification and validation of hub genes in drug induced acute kidney injury basing on integrated transcriptomic analysis. Frontiers in Immunology, 2023 (PubMed: 37063876) [IF=7.3]
3). Tumor necrosis factor-stimulated gene-6 ameliorates early brain injury after subarachnoid hemorrhage by suppressing NLRC4 inflammasome-mediated astrocyte pyroptosis. Neural Regeneration Research, 2024 (PubMed: 37862209) [IF=6.1]

Application: WB    Species: Mouse    Sample:

Figure 1 Representative photographs of SAH mice and the expression levels of TSG-6, NLRC4, GSDMD, and GSDMD-N after SAH. (A) Representative photographs of a sham and subarachnoid hemorrhage (SAH) mouse brain 24 hours after model construction. (B) Representative photographs of hematoxylin and eosin-stained slides of sham cerebral cortex and cortex 24 hours after SAH. Scale bar: 50 μm. (C, E) Western blot bands and quantification of TSG-6, NLRC4, GSDMD, and GSDMD-N proteins in sham mice and at 6, 12, 24, 48, and 72 hours after SAH. (D) Representative immunohistochemistry staining results for TSG-6 in the cortex of sham and SAH mice 24 hours after model construction. Scale bar: 50 μm. Values shown are means ± standard deviation of 6 samples from each group. **P < 0.01, ***P < 0.001, vs. sham group (one-way analysis of variance with Tukey’s honestly significant difference test). GSDMD: Gasdermin-D; NLRC4: NLR family CARD domain containing 4; SAH: subarachnoid hemorrhage; TSG-6: Tumor necrosis factor-stimulated gene-6; WB: western blot.

Application: IF/ICC    Species: Mouse    Sample:

Figure 2 TSG-6 is expressed in astrocytes and neurons after subarachnoid hemorrhage (SAH). (A) Representative co-immunofluorescence (IF) images showing TSG-6 (green) in astrocytes (GFAP, red) of sham mice. (B) Representative co-IF images showing TSG-6 (green) in astrocytes (GFAP, red), microglia (Iba-1, red), and neurons (NeuN, red) of SAH mice 24 hours after SAH. Scale bars: 50 μm. (C, D) Quantification of IF staining results. Values shown are means ± standard deviation of 6 samples from each group. ****P < 0.0001 (one-way analysis of variance with Tukey’s honestly significant difference test [D] or Student’s t-test [C]). DAPI: 4′,6-Diamidino-2-phenylindole; GFAP: glial fibrillary acidic protein; Iba-1: ionized calcium-binding adapter molecule 1; IF: immunofluorescence; NeuN: neuronal nuclei antigen; SAH: subarachnoid hemorrhage; TSG-6: Tumor necrosis factor-stimulated gene-6.
4). Identification of Neural Progenitor Cell-associated Chemoradiotherapy Resistance Gene Set (ARL4C, MSN, TNFAIP6) for Prognosis of Glioma. Current Pharmaceutical Design, 2019 [IF=3.1]

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