DNMT3B Antibody - #AF5493
(4)
(8)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF5493, RRID:AB_2837973.
折りたたみ／展開
Cytosine 5methyltransferase 3B; DNA; DNA (cytosine 5) methyltransferase 3 beta; DNA (cytosine 5)-methyltransferase 3B; DNA (cytosine-5)-methyltransferase 3B; DNA methyltransferase HsaIIIB; DNA MTase HsaIIIB; DNM3B_HUMAN; Dnmt3b; EC 2.1.1.37; ICF; ICF1; M.HsaIIIB; MGC124407; RP23-89H14.3;
免疫原
Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate.
- Q9UBC3 DNM3B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKGDTRHLNGEEDAGGREDSILVNGACSDQSSDSPPILEAIRTPEIRGRRSSSRLSKREVSSLLSYTQDLTGDGDGEDGDGSDTPVMPKLFRETRTRSESPAVRTRNNNSVSSRERHRPSPRSTRGRQGRNHVDESPVEFPATRSLRRRATASAGTPWPSPPSSYLTIDLTDDTEDTHGTPQSSSTPYARLAQDSQQGGMESPQVEADSGDGDSSEYQDGKEFGIGDLVWGKIKGFSWWPAMVVSWKATSKRQAMSGMRWVQWFGDGKFSEVSADKLVALGLFSQHFNLATFNKLVSYRKAMYHALEKARVRAGKTFPSSPGDSLEDQLKPMLEWAHGGFKPTGIEGLKPNNTQPVVNKSKVRRAGSRKLESRKYENKTRRRTADDSATSDYCPAPKRLKTNCYNNGKDRGDEDQSREQMASDVANNKSSLEDGCLSCGRKNPVSFHPLFEGGLCQTCRDRFLELFYMYDDDGYQSYCTVCCEGRELLLCSNTSCCRCFCVECLEVLVGTGTAAEAKLQEPWSCYMCLPQRCHGVLRRRKDWNVRLQAFFTSDTGLEYEAPKLYPAIPAARRRPIRVLSLFDGIATGYLVLKELGIKVGKYVASEVCEESIAVGTVKHEGNIKYVNDVRNITKKNIEEWGPFDLVIGGSPCNDLSNVNPARKGLYEGTGRLFFEFYHLLNYSRPKEGDDRPFFWMFENVVAMKVGDKRDISRFLECNPVMIDAIKVSAAHRARYFWGNLPGMNRPVIASKNDKLELQDCLEYNRIAKLKKVQTITTKSNSIKQGKNQLFPVVMNGKEDVLWCTELERIFGFPVHYTDVSNMGRGARQKLLGRSWSVPVIRHLFAPLKDYFACE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UBC3 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S51 | Phosphorylation | Uniprot | |
| S52 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| T96 | Phosphorylation | Uniprot | |
| S98 | Phosphorylation | Uniprot | |
| S100 | Phosphorylation | Uniprot | |
| T105 | Phosphorylation | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| S112 | Phosphorylation | Uniprot | |
| S113 | Phosphorylation | Uniprot | |
| S120 | Phosphorylation | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| S195 | Phosphorylation | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| S209 | Phosphorylation | Uniprot | |
| S270 | Phosphorylation | Uniprot | |
| Y303 | Phosphorylation | Uniprot | |
| S320 | Phosphorylation | Uniprot | |
| T353 | Phosphorylation | Uniprot | |
| T383 | Phosphorylation | Uniprot | |
| S387 | Phosphorylation | Uniprot | |
| K397 | Ubiquitination | Uniprot | |
| T401 | Phosphorylation | Uniprot | |
| Y404 | Phosphorylation | Uniprot | |
| S416 | Phosphorylation | Uniprot | |
| K428 | Ubiquitination | Uniprot | |
| K562 | Ubiquitination | Uniprot | |
| Y588 | Phosphorylation | Uniprot | |
| K597 | Ubiquitination | Uniprot | |
| K623 | Ubiquitination | Uniprot | |
| K662 | Ubiquitination | Uniprot | |
| S711 | Phosphorylation | Uniprot | |
| K777 | Ubiquitination | Uniprot | |
| S780 | Phosphorylation | Uniprot | |
| Y815 | Phosphorylation | Uniprot | |
| T816 | Phosphorylation | Uniprot | |
| S819 | Phosphorylation | Uniprot | |
| R823 | Methylation | Uniprot | |
| R826 | Methylation | Uniprot | |
| S835 | Phosphorylation | Uniprot | |
| K847 | Ubiquitination | Uniprot |
研究背景
Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Functions as a transcriptional corepressor by associating with ZHX1. Required for DUX4 silencing in somatic cells.
Sumoylated.
Citrullinated by PADI4.
Nucleus.
Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate.
Interacts with BAZ2A/TIP5, SUV39H1 and CBX4. Interacts with UHRF1 (By similarity). Interacts with DNMT1 and DNMT3A, SETDB1, UBL1, UBE2I9 and ZHX1. Interacts with the PRC2/EED-EZH2 complex.
The PWWP domain is essential for targeting to pericentric heterochromatin.
Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.
研究領域
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
参考文献
Application: WB Species: human Sample: HCT-8 and HCT-8/Fu cells
Application: WB Species: rat Sample: ovaries
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