BMI1 Antibody - #DF6017
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| 製品: | BMI1 Antibody |
| カタログ: | DF6017 |
| タンパク質の説明: | Rabbit polyclonal antibody to BMI1 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Sheep, Rabbit, Chicken |
| 分子量: | 37kDa; 37kD(Calculated). |
| ユニプロット: | P35226 |
| RRID: | AB_2837992 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6017, RRID:AB_2837992.
折りたたみ／展開
B lymphoma Mo MLV insertion region (mouse); B lymphoma Mo MLV insertion region 1 homolog; Bmi 1; BMI1; BMI1 polycomb ring finger oncogene; BMI1_HUMAN; Flvi 2/bmi 1; FLVI2/BMI1; MGC12685; Murine leukemia viral (bmi 1) oncogene homolog; Oncogene BMI 1; PCGF 4; PCGF4; Polycomb complex protein BMI 1; Polycomb complex protein BMI-1; Polycomb group protein Bmi1; Polycomb group ring finger 4; Polycomb group RING finger protein 4; RING finger protein 51; RNF 51; RNF51;
免疫原
- P35226 BMI1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MHRTTRIKITELNPHLMCVLCGGYFIDATTIIECLHSFCKTCIVRYLETSKYCPICDVQVHKTRPLLNIRSDKTLQDIVYKLVPGLFKNEMKRRRDFYAAHPSADAANGSNEDRGEVADEDKRIITDDEIISLSIEFFDQNRLDRKVNKDKEKSKEEVNDKRYLRCPAAMTVMHLRKFLRSKMDIPNTFQIDVMYEEEPLKDYYTLMDIAYIYTWRRNGPLPLKYRVRPTCKRMKISHQRDGLTNAGELESDSGSDKANSPAGGIPSTSSCLPSPSTPVQSPHPQFPHISSTMNGTSNSPSGNHQSSFANRPRKSSVNGSSATSSG
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P35226 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| K73 | Ubiquitination | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| K88 | Sumoylation | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| R114 | Methylation | Uniprot | |
| K151 | Acetylation | Uniprot | |
| Y203 | Phosphorylation | Uniprot | |
| Y204 | Phosphorylation | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| T244 | Phosphorylation | Uniprot | |
| S251 | Phosphorylation | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| S260 | Phosphorylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| T277 | Phosphorylation | Uniprot | |
| S281 | Phosphorylation | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | P31749 (AKT1) | Uniprot |
研究背景
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A. In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2.
Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.
Nucleus. Cytoplasm.
Component of a PRC1-like complex. Identified in a PRC1-like HPRC-H complex with CBX2, CBX4, CBX8, PHC1, PHC2, PHC3 RING1 and RNF2. Interacts with RNF2/RING2. Interacts with RING1 (By similarity). Part of a complex that contains RNF2, UB2D3 and BMI1, where RNF2 and BMI1 form a tight heterodimer, and UB2D3 interacts only with RNF2. The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A. Interacts with CBX7 and CBX8. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1. Interacts with PHC2.
研究領域
· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
参考文献
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