MTM1 Antibody - #DF6058
| 製品: | MTM1 Antibody |
| カタログ: | DF6058 |
| タンパク質の説明: | Rabbit polyclonal antibody to MTM1 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Rabbit, Dog, Xenopus |
| 分子量: | 70kDa; 70kD(Calculated). |
| ユニプロット: | Q13496 |
| RRID: | AB_2838027 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6058, RRID:AB_2838027.
折りたたみ／展開
AF073996; CG2; CNM; KIAA4176; mKIAA4176; Mtm; Mtm1; MTM1_HUMAN; MTMX; Myotubular myopathy 1; Myotubularin; XLMTM;
免疫原
- Q13496 MTM1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASASTSKYNSHSLENESIKRTSRDGVNRDLTEAVPRLPGETLITDKEVIYICPFNGPIKGRVYITNYRLYLRSLETDSSLILDVPLGVISRIEKMGGATSRGENSYGLDITCKDMRNLRFALKQEGHSRRDMFEILTRYAFPLAHSLPLFAFLNEEKFNVDGWTVYNPVEEYRRQGLPNHHWRITFINKCYELCDTYPALLVVPYRASDDDLRRVATFRSRNRIPVLSWIHPENKTVIVRCSQPLVGMSGKRNKDDEKYLDVIRETNKQISKLTIYDARPSVNAVANKATGGGYESDDAYHNAELFFLDIHNIHVMRESLKKVKDIVYPNVEESHWLSSLESTHWLEHIKLVLTGAIQVADKVSSGKSSVLVHCSDGWDRTAQLTSLAMLMLDSFYRSIEGFEILVQKEWISFGHKFASRIGHGDKNHTDADRSPIFLQFIDCVWQMSKQFPTAFEFNEQFLIIILDHLYSCRFGTFLFNCESARERQKVTERTVSLWSLINSNKEKFKNPFYTKEINRVLYPVASMRHLELWVNYYIRWNPRIKQQQPNPVEQRYMELLALRDEYIKRLEELQLANSAKLSDPPTSPSSPSQMMPHVQTHF
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13496 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K8 | Ubiquitination | Uniprot | |
| Y9 | Phosphorylation | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| K20 | Ubiquitination | Uniprot | |
| Y71 | Phosphorylation | Uniprot | |
| S91 | Phosphorylation | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| S106 | Phosphorylation | Uniprot | |
| Y107 | Phosphorylation | Uniprot | |
| T112 | Phosphorylation | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| S147 | Phosphorylation | Uniprot | |
| K236 | Ubiquitination | Uniprot | |
| K252 | Ubiquitination | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| Y260 | Phosphorylation | Uniprot | |
| K273 | Ubiquitination | Uniprot | |
| Y277 | Phosphorylation | Uniprot | |
| Y295 | Phosphorylation | Uniprot | |
| S297 | Phosphorylation | Uniprot | |
| Y301 | Phosphorylation | Uniprot | |
| T495 | Phosphorylation | Uniprot | |
| K546 | Ubiquitination | Uniprot | |
| Y557 | Phosphorylation | Uniprot | |
| T587 | Phosphorylation | Uniprot | |
| S588 | Phosphorylation | Uniprot | |
| S590 | Phosphorylation | Uniprot | |
| S591 | Phosphorylation | Uniprot |
研究背景
Lipid phosphatase which dephosphorylates phosphatidylinositol 3-monophosphate (PI3P) and phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2). Has also been shown to dephosphorylate phosphotyrosine- and phosphoserine-containing peptides. Negatively regulates EGFR degradation through regulation of EGFR trafficking from the late endosome to the lysosome. Plays a role in vacuolar formation and morphology. Regulates desmin intermediate filament assembly and architecture. Plays a role in mitochondrial morphology and positioning. Required for skeletal muscle maintenance but not for myogenesis. In skeletal muscles, stabilizes MTMR12 protein levels.
Cytoplasm. Cell membrane>Peripheral membrane protein. Cell projection>Filopodium. Cell projection>Ruffle. Late endosome. Cytoplasm>Myofibril>Sarcomere.
Note: Localizes as a dense cytoplasmic network (PubMed:11001925). Also localizes to the plasma membrane, including plasma membrane extensions such as filopodia and ruffles (PubMed:12118066). Predominantly located in the cytoplasm following interaction with MTMR12 (PubMed:12847286). Recruited to the late endosome following EGF stimulation (PubMed:14722070). In skeletal muscles, co-localizes with MTMR12 in the sarcomere (By similarity).
Heterodimer with MTMR12. Interacts with KMT2A/MLL1 (via SET domain). Interacts with DES in skeletal muscle but not in cardiac muscle. Interacts with SPEG.
The GRAM domain mediates binding to PI(3,5)P2 and, with lower affinity, to other phosphoinositides.
Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.
研究領域
· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.
· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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