NME1 Antibody - #DF6062
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6062, RRID:AB_2838031.
折りたたみ／展開
AWD; AWD, drosophila, homolog of; GAAD; Granzyme A activated DNase; Granzyme A-activated DNase; GZMA activated DNase; Metastasis inhibition factor NM23; NB; NBS; NDK A; NDKA; NDKA_HUMAN; NDP kinase A; NDPK-A; NDPKA; NM23; NM23 long variant, included; nm23-H1; NM23-M1; NM23H1B, included; NME/NM23 nucleoside diphosphate kinase 1; Nme1; NME1-NME2 spliced read-through transcript, included; Non-metastatic cells 1, protein (NM23A) expressed in; Nonmetastatic cells 1, protein expressed in; Nonmetastatic protein 23; Nonmetastatic protein 23, homolog 1; Nucleoside diphosphate kinase A; Tumor metastatic process-associated protein;
免疫原
Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation.
- P15531 NDKA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MANCERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVGLKFMQASEDLLKEHYVDLKDRPFFAGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVESAEKEIGLWFHPEELVDYTSCAQNWIYE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P15531 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R6 | Methylation | Uniprot | |
| K12 | Acetylation | Uniprot | |
| K12 | Ubiquitination | Uniprot | |
| R18 | Methylation | Uniprot | |
| K26 | Acetylation | Uniprot | |
| K26 | Ubiquitination | Uniprot | |
| K31 | Acetylation | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| K39 | Ubiquitination | Uniprot | |
| S44 | Phosphorylation | P15531 (NME1) | Uniprot |
| K49 | Acetylation | Uniprot | |
| K49 | Sumoylation | Uniprot | |
| K49 | Ubiquitination | Uniprot | |
| Y52 | Phosphorylation | Uniprot | |
| K56 | Acetylation | Uniprot | |
| K56 | Sumoylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| R58 | Methylation | Uniprot | |
| K66 | Acetylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| Y67 | Phosphorylation | Uniprot | |
| K85 | Acetylation | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| R88 | Methylation | Uniprot | |
| T94 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S99 | Phosphorylation | Uniprot | |
| K100 | Acetylation | Uniprot | |
| K100 | Methylation | Uniprot | |
| K100 | Sumoylation | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| T103 | Phosphorylation | Uniprot | |
| H118 | Phosphorylation | P15531 (NME1) | Uniprot |
| S120 | Phosphorylation | P15531 (NME1) , P06493 (CDK1) | Uniprot |
| S122 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| S125 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
PTMs - P15531 酵素として
| Substrate | Site | Source |
|---|---|---|
| P15531 (NME1) | S44 | Uniprot |
| P15531-2 (NME1) | S69 | Uniprot |
| P15531 (NME1) | H118 | Uniprot |
| P15531 (NME1) | S120 | Uniprot |
| P15531-1 (NME1) | S122 | Uniprot |
| P15531-1 (NME1) | S125 | Uniprot |
| P15531-2 (NME1) | S145 | Uniprot |
| P15531-2 (NME1) | S147 | Uniprot |
| P15531-2 (NME1) | S150 | Uniprot |
| Q8IVT5 (KSR1) | S406 | Uniprot |
| Q969H4-2 (CNKSR1) | S385 | Uniprot |
研究背景
Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.
Cytoplasm. Nucleus.
Note: Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA.
Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation.
Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). Interacts with PRUNE1. Component of the SET complex, composed of at least ANP32A, APEX1, HMGB2, NME1, SET and TREX1. Within this complex, interacts directly with SET. Also interacts with TREX1, but only following translocation to the nucleus.
Belongs to the NDK family.
研究領域
· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.
· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.
· Metabolism > Global and overview maps > Metabolic pathways.
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