IGF1 Antibody - #DF6096

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製品: IGF1 Antibody
カタログ: DF6096
タンパク質の説明: Rabbit polyclonal antibody to IGF1
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 17kDa; 22kD(Calculated).
ユニプロット: P05019
RRID: AB_2838064

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関連ダウンロード
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(86%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
IGF1 Antibody detects endogenous levels of total IGF1.
RRID:
AB_2838064
引用形式: Affinity Biosciences Cat# DF6096, RRID:AB_2838064.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

IBP1; IGF I; IGF IA; IGF IB; IGF-I; Igf1; IGF1_HUMAN; IGF1A; IGFI; IGFIA; Insulin like growth factor 1 (somatomedin C); Insulin like growth factor 1; Insulin like growth factor IA; Insulin like growth factor IB; Insulin-like growth factor I; Mechano growth factor; MGF; OTTHUMP00000195080; OTTHUMP00000195081; OTTHUMP00000195082; OTTHUMP00000195083; OTTHUMP00000195084; Somatomedia C; Somatomedin C; Somatomedin-C;

免疫原

免疫原:
Uniprot:

P05019(IGF1_HUMAN) >>Visit HPA database.

遺伝子(ID):
IGF1(3479)
タンパク質の説明:
IGF1, also named as IBP1, MGF, IGF-IA and Somatomedin-C, belongs to the insulin family. IGF1 is structurally and functionally related to insulin but have a much higher growth-promoting activity. Altered expression or mutation of IGF-1 is associated with several human disorders, including type I diabetes and various forms of cancer. Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. The antibody is specific to isoform IGF-1A.
タンパク質配列:
MGKISSLPTQLFKCCFCDFLKVKMHTMSSSHLFYLALCLLTFTSSATAGPETLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPAKSARSVRAQRHTDMPKTQKYQPPSTNKNTKSQRRKGWPKTHPGGEQKEGTEASLQIRGKKKEQRREIGSRNAECRGKKGK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
86
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05019 基板として

Site PTM Type Enzyme
K134 Ubiquitination
Y135 Phosphorylation
S184 Phosphorylation

研究背景

機能:

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb (By similarity). Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Forms a ternary complex with IGFR1 and ITGAV:ITGB3. Forms a ternary complex with IGFR1 and ITGA6:ITGB4.

タンパク質ファミリー:

Belongs to the insulin family.

研究領域

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Nervous system > Long-term depression.

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.

参考文献

1). Cathepsin S activity controls chronic stress-induced muscle atrophy and dysfunction in mice. Cellular and Molecular Life Sciences, 2023 (PubMed: 37589754) [IF=8.0]

Application: WB    Species: Mouse    Sample:

Fig. 4 CTSS deficiency ameliorated stress-related anabolic and catabolic molecular alterations. a–e: Representative immunoblotting images and quantitative data for CTSS, IGF-1, IRS-2, p-PI3K, p-Akt, p-mTOR, p-FoxO1α, MuRF-1, MAFbx1, PGC-1α, PPAR-γ, C-caspase-3, and Bcl-2 in GAS muscles at Day 14 after stress (n = 3). Data are mean ± SEM, and p-values were determined by a one-way ANOVA followed by Bonferroni post hoc tests (b–e). CW: CTSS+/+ control mice, CK: CTSS−/− control mice, SW: 14-day-stressed CTSS+/+ mice, SK: 14-day-stressed CTSS−/− mice. *p 
2). An IGF1-expressing endometrial stromal cell population is associated with human decidualization. BMC Biology, 2022 (PubMed: 36482461) [IF=5.4]

Application: IHC    Species: Human    Sample:

Fig. 7 The aberrant ratio of IGF1+SC to IGF1R+SC is observed in repeated implantation failure patients. A Cell phone heatmap showed the interaction of cells. The number indicates the number of interactions. B Heat map showing relative expression (z-score) of IGF1 and IGF1R in the total Rem_SC (Rem_SC and dRem_SC) from Endometrium and Decidua. C Heat map showing relative expression (z-score) of IGF1 and IGF1R in the stroma cells from Ctrl and RIF. D, E The expression of IGF1 and IGF1R in the endometrial stroma from controls and RIF patients at the WOI time were analyzed by immunohistochemistry (n = 3 for each group). Scale bar, 20 μm. F General diagram of decidualization process. During the time of WOI, the heterogeneity of stromal cells determines different division of labor. More specifically, IGF1+ stromal cells (Rem) start endometrial decidualization (mainly IGF1R+ stromal cells, i.e., dRem and dSec) by crosstalking with EVT and immune cells, including AREG+ NK cells; IL1B+ stromal cells (dSec) participate in the establishment and maintenance of decidua hemostasis.
3). Cardiomyocyte protective effects of thyroid hormone during hypoxia/reoxygenation injury through activating of IGF‐1‐mediated PI3K/Akt signalling. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2021 (PubMed: 33724692) [IF=5.3]

Application: WB    Species: mouse    Sample: cardiomyocytes

FIGURE 6| T3 protected cardiomyocytes from H/R-induced apoptosis through PI3K/Akt signalling pathway (A and B). A, The expression of Akt, P-Akt, PI3K, P-Akt, IGF-1, IGF-1R; β-actin protein expression was used for normalization and (B) representative bands quantified in the corresponding bar graph.
4). WISP2/IGF-1 promotes the survival of DSCs and impair the cytotoxicity of decidual NK cells. REPRODUCTION, 2021 (PubMed: 33561006) [IF=3.8]
5). Capsaicin reduces blood glucose and prevents prostate growth by regulating androgen, RAGE/IGF-1/Akt, TGF-β/Smad signalling pathway and reversing epithelial-mesenchymal transition in streptozotocin-induced diabetic mice. Naunyn-Schmiedeberg's archives of pharmacology, 2024 (PubMed: 38700794) [IF=3.6]
6). OSI-906 restores the sensitivity of ovarian clear cell carcinoma to cisplatin by targeting the IGF1R/AKT pathway. MEDICAL ONCOLOGY, 2022 (PubMed: 34982265) [IF=3.4]
7). Novel_circ_003686 regulates the osteogenic differentiation of MSCs in patients with myeloma bone disease through miR-142-5p/IGF1 axis. Journal of bone oncology, 2023 (PubMed: 38021072) [IF=3.4]

Application: WB    Species: Human    Sample:

Fig. 5 Regulatory relationship between miR-142-3p and IGF1. (A) Online TargetScan (https://www.targetscan.org/vert_72/) suggests that miR-142-3p may bind to 3′ untranslated region (3′UTR) of IGF1 mRNA. (B) The binding of miR-142-3p to IGF1 was verified by dual-luciferase reporter assay. (C) IGF1 mRNA expression after miR-142-3p-mimic treatment was examined by RT-PCR analysis. (D-E) The IGF1 was silencing by si-IGF1-1, si-IGF1-2 or si-IGF1-3 in MBD-BMSC, and the protein expression was observed by WB. (F) IGF1 mRNA expression after IGF1 was silenced was examined by RT-PCR. * P < 0.05, ** P < 0.01 and *** P < 0.001.
8). Downregulated lncRNA HOTAIR ameliorates polycystic ovaries syndrome via IGF-1 mediated PI3K/Akt pathway. Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology, 2023 (PubMed: 37356454) [IF=2.0]
9). Effects of high-dose all-trans retinoic acid on longitudinal bone growth of young rats. GROWTH HORMONE & IGF RESEARCH, 2022 (PubMed: 35149382) [IF=1.4]
10). RCC2 Expression Stimulates ER-Positive Breast Tumorigenesis. Journal of Oncology, 2020 (PubMed: 32565805)

Application: WB    Species:    Sample: MCF-7 cells

Figure 3:| Assessment of the protein levels of IGF1 and TWIST1 in MCF-7 cells using Western blot analysis. MCF-7 cells were transfected with anti-RCC2 siRNA (si-RCC2) (a–c) or RCC2-expressing plasmids (O-RCC2) (d–f ).
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