P4HB Antibody - #DF6131
製品: | P4HB Antibody |
カタログ: | DF6131 |
タンパク質の説明: | Rabbit polyclonal antibody to P4HB |
アプリケーション: | WB IHC |
反応性: | Human, Mouse, Rat |
予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
分子量: | 57kDa; 57kD(Calculated). |
ユニプロット: | P07237 |
RRID: | AB_2838098 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6131, RRID:AB_2838098.
折りたたみ/展開
Cellular thyroid hormone binding protein; Cellular thyroid hormone-binding protein; Collagen prolyl 4 hydroxylase beta; Disulphide Isomerase; DSI; EC 5.3.4.1; Endoplasmic reticulum resident protein 59; ER protein 59; ERBA2L; ERp59; GIT; Gltathione insulin transhydrogenase; Glutathione insulin transhydrogenase; P4HB; P4Hbeta; p55; PDI; PDIA1; PDIA1_HUMAN; PDIR; PHDB; PO4DB; PO4HB; Procollagen proline 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase) beta polypeptide (protein disulfide isomerase associated 1); Procollagen proline 2 oxoglutarate 4 dioxygenase beta subunit; PROHB; Prolyl 4 hydroxylase beta polypeptide; Prolyl 4 hydroxylase beta subunit; Prolyl 4 hydroxylase subunit beta; Prolyl 4-hydroxylase subunit beta; Protein disulfide isomerase associated 1; Protein disulfide isomerase, family A, member 1; Protein disulfide isomerase/oxidoreductase; Protein disulfide-isomerase; Protocollagen hydroxylase; Thbp; Thyroid hormone binding protein p55; Thyroid hormone binding protein p55 cellular; V erb a avian erythroblastic leukemia viral oncogene homolog 2 like;
免疫原
- P07237 PDIA1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLRRALLCLAVAALVRADAPEEEDHVLVLRKSNFAEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTAGREADDIVNWLKKRTGPAATTLPDGAAAESLVESSEVAVIGFFKDVESDSAKQFLQAAEAIDDIPFGITSNSDVFSKYQLDKDGVVLFKKFDEGRNNFEGEVTKENLLDFIKHNQLPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYDGKLSNFKTAAESFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESEELTAERITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDDDDLEDLEEAEEPDMEEDDDQKAVKDEL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P07237 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
K31 | Ubiquitination | Uniprot | |
S32 | Phosphorylation | Uniprot | |
C53 | S-Nitrosylation | Uniprot | |
C56 | S-Nitrosylation | Uniprot | |
Y63 | Phosphorylation | Uniprot | |
K65 | Acetylation | Uniprot | |
K65 | Ubiquitination | Uniprot | |
R78 | Methylation | Uniprot | |
K81 | Ubiquitination | Uniprot | |
S88 | Phosphorylation | Uniprot | |
Y94 | Phosphorylation | Uniprot | |
R97 | Methylation | Uniprot | |
Y99 | Phosphorylation | Uniprot | |
K103 | Acetylation | Uniprot | |
K103 | Ubiquitination | Uniprot | |
R106 | Methylation | Uniprot | |
K130 | Methylation | Uniprot | |
K130 | Ubiquitination | Uniprot | |
K200 | Ubiquitination | Uniprot | |
K208 | Acetylation | Uniprot | |
K222 | Ubiquitination | Uniprot | |
S266 | Phosphorylation | Uniprot | |
Y268 | Phosphorylation | Uniprot | |
K271 | Ubiquitination | Uniprot | |
S273 | Phosphorylation | Uniprot | |
S281 | Phosphorylation | Uniprot | |
K283 | Acetylation | Uniprot | |
S293 | Phosphorylation | Uniprot | |
K308 | Acetylation | Uniprot | |
K308 | Ubiquitination | Uniprot | |
C312 | S-Nitrosylation | Uniprot | |
K326 | Ubiquitination | Uniprot | |
Y327 | Phosphorylation | Uniprot | |
K328 | Ubiquitination | Uniprot | |
S331 | Phosphorylation | Uniprot | |
T335 | Phosphorylation | Uniprot | |
R338 | Methylation | Uniprot | |
S357 | Phosphorylation | Uniprot | |
K375 | Acetylation | Uniprot | |
K375 | Ubiquitination | Uniprot | |
K385 | Acetylation | Uniprot | |
K385 | Ubiquitination | Uniprot | |
C397 | S-Nitrosylation | Uniprot | |
C400 | S-Nitrosylation | Uniprot | |
K409 | Acetylation | Uniprot | |
K415 | Ubiquitination | Uniprot | |
K424 | Ubiquitination | Uniprot | |
S427 | Phosphorylation | Uniprot | |
T428 | Phosphorylation | Uniprot | |
S439 | Phosphorylation | Uniprot | |
T442 | O-Glycosylation | Uniprot | |
K444 | Acetylation | Uniprot | |
K444 | Ubiquitination | Uniprot | |
R452 | Methylation | Uniprot | |
Y457 | Phosphorylation | Uniprot | |
T462 | Phosphorylation | Uniprot | |
K467 | Methylation | Uniprot | |
K467 | Ubiquitination | Uniprot |
研究背景
This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.
Endoplasmic reticulum. Endoplasmic reticulum lumen. Melanosome. Cell membrane>Peripheral membrane protein.
Note: Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources (Probable). Localizes near CD4-enriched regions on lymphoid cell surfaces (PubMed:11181151). Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:10636893). Colocalizes with MTTP in the endoplasmic reticulum (PubMed:23475612).
Heterodimer; heterodimerizes with the protein microsomal triglyceride transfer MTTP. Homodimer. Monomers and homotetramers may also occur. Also constitutes the structural subunit of prolyl 4-hydroxylase and of the microsomal triacylglycerol transfer protein MTTP in mammalian cells. Stabilizes both enzymes and retain them in the ER without contributing to the catalytic activity (By similarity). Binds UBQLN1. Interacts with ERO1B. Binds to CD4, and upon HIV-1 binding to the cell membrane, is part of a P4HB/PDI-CD4-CXCR4-gp120 complex.
Belongs to the protein disulfide isomerase family.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
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