Caspase 1 Antibody - #DF6148

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製品: Caspase 1 Antibody
カタログ: DF6148
タンパク質の説明: Rabbit polyclonal antibody to Caspase 1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Rabbit
分子量: 45kDa; 45kD(Calculated).
ユニプロット: P29466
RRID: AB_2838115

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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Rabbit(100%)
クローナリティ:
Polyclonal
特異性:
Caspase 1 Antibody detects endogenous levels of total Caspase 1.
RRID:
AB_2838115
引用形式: Affinity Biosciences Cat# DF6148, RRID:AB_2838115.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CASP-1; CASP1; CASP1_HUMAN; Caspase 1; Caspase-1 subunit p10; ICE; IL-1 beta-converting enzyme; IL-1BC; IL1 beta converting enzyme; IL1B convertase; Interleukin 1 beta convertase; Interleukin 1B converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45;

免疫原

免疫原:
Uniprot:

P29466(CASP1_HUMAN) >>Visit HPA database.

遺伝子(ID):
CASP1(834)
発現特異性:
P29466 CASP1_HUMAN:

Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.

タンパク質の説明:
Caspase-1, or interleukin-1 converting enzyme (ICE/ICEα), is a class I cysteine protease, which also includes caspases -4, -5, -11, and -12. Caspase-1 cleaves inflammatory cytokines such as pro-IL-1 and interferon-γ inducing factor (IL-18) into their mature forms (1,2). Like other caspases, caspase-1 is proteolytically activated from a proenzyme to produce a tetramer of its two active subunits, p20 and p10. Caspase-1 has a large amino-terminal pro-domain that contains a caspase recruitment domain (CARD). Overexpression of caspase-1 can induce apoptosis (3). Mice deficient in caspase-1, however, have no overt defects in apoptosis but do have defects in the maturation of pro-IL-1β and are resistant to endotoxic shock (4,5). At least six caspase-1 isoforms have been identified, including caspase-1 α, β, γ, δ, ε and ζ (6). Most caspase-1 isoforms (α, β, γ and δ) produce products between 30-48 kDa and induce apoptosis upon over-expression. Caspase-1 ε typically contains only the p10 subunit, does not induce apoptosis and may act as a dominant negative. The widely expressed ζ isoform of caspase-1 induces apoptosis and lacks 39 amino-terminal residues found in the α isoform (6). Activation of caspase-1 occurs through an oligomerization molecular platform designated the inflammasome that includes caspase-5, Pycard/Asc, and NALP1 (7).
タンパク質配列:
MADKVLKEKRKLFIRSMGEGTINGLLDELLQTRVLNKEEMEKVKRENATVMDKTRALIDSVIPKGAQACQICITYICEEDSYLAGTLGLSADQTSGNYLNMQDSQGVLSSFPAPQAVQDNPAMPTSSGSEGNVKLCSLEEAQRIWKQKSAEIYPIMDKSSRTRLALIICNEEFDSIPRRTGAEVDITGMTMLLQNLGYSVDVKKNLTASDMTTELEAFAHRPEHKTSDSTFLVFMSHGIREGICGKKHSEQVPDILQLNAIFNMLNTKNCPSLKDKPKVIIIQACRGDSPGVVWFKDSVGVSGNLSLPTTEEFEDDAIKKAHIEKDFIAFCSSTPDNVSWRHPTMGSVFIGRLIEHMQEYACSCDVEEIFRKVRFSFEQPDGRAQMPTTERVTLTRCFYLFPGH

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Rabbit
100
Horse
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P29466 基板として

Site PTM Type Enzyme
S16 Phosphorylation
T21 Phosphorylation
T32 Phosphorylation
K37 Ubiquitination
K44 Ubiquitination
T49 Phosphorylation
K53 Ubiquitination
K134 Ubiquitination
K148 Ubiquitination
S149 Phosphorylation
K158 Ubiquitination
K204 Ubiquitination
T226 Phosphorylation
S227 Phosphorylation
K268 Ubiquitination
K274 Ubiquitination
K278 Ubiquitination
S306 Phosphorylation
K319 Ubiquitination
K320 Ubiquitination
K325 Ubiquitination
S376 Phosphorylation

研究背景

機能:

Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive. In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly.

PTMs:

The two subunits are derived from the precursor sequence by an autocatalytic mechanism.

細胞の位置付け:

Cytoplasm. Cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.

サブユニット構造:

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Both the p10 and p20 subunits interact with MEFV. Interacts with CARD17/INCA and CARD18. Interacts with SERPINB1; this interaction regulates CASP1 activity.

タンパク質ファミリー:

Belongs to the peptidase C14A family.

研究領域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

参考文献

1). 20(S)- Protopanaxadiol saponins isolated from Panax notoginseng target the binding of HMGB1 to TLR4 against inflammation in experimental ulcerative colitis. Phytotherapy Research, 2023 (PubMed: 37424151) [IF=7.2]
2). Berberine attenuates uric acid-induced cell injury by inhibiting NLRP3 signaling pathway in HK-2 cells. Naunyn-Schmiedeberg's Archives of Pharmacology, 2023 (PubMed: 37193772) [IF=3.6]

Application: WB    Species: Human    Sample: HK-2 cells

Fig. 6 Effect of BBR on NLRP3 pathway in HK-2 cells using Western blot. A Western blot analyses of NLRP3, ASC, cl-Caspase1, Caspase1, IL-18, IL-1β, GSDMD, and β-actin. B–H Intensity of NLRP3, ASC, cl-Caspase1, Caspase1, IL-18, IL-1β, and GSDMD relative to β-actin. Values represent the means ± SD (n = 3). BBR, berberine (20 μM); UA, uric acid (20 mg/dL); cl-Caspase1, cleaved-Caspase1.
3). Polygalasaponin F ameliorates middle cerebral artery occlusion-induced focal ischemia / reperfusion injury in rats through inhibiting TXNIP/NLRP3 signaling pathway. Journal of neuroimmunology, 2024 (PubMed: 38198981) [IF=3.3]
4). Sulforaphane Ameliorates Limb Ischemia/Reperfusion-Induced Muscular Injury in Mice by Inhibiting Pyroptosis and Autophagy via the Nrf2-ARE Pathway. Evidence-Based Complementary and Alternative Medicine, 2022 (PubMed: 35600947)

Application: WB    Species: Mice    Sample:

Figure 4 (a) Immunofluorescent staining labeled autophagic markers Beclin-1 and LC3 was conducted to assess the extent of autophagy in each group of mice (scale bar = 50 μm); (b) Western blot was used to examine the expression of autophagy markers in the mouse muscle homogenate. The experiments were repeated three times. ∗p < 0.05, ∗∗p < 0.01.
5). XueFu ZhuYu Decoction Alleviates Cardiopulmonary Bypass-Induced NLRP3 Inflammasome-Dependent Pyroptosis by Inhibiting IkB-α/NF-κB Pathway in Acute Lung Injury Rats. Evidence-based Complementary and Alternative Medicine, 2022 (PubMed: 36124015)

Application: IF/ICC    Species: Rat    Sample: lung tissues

Figure 6 XFZYD decreased pyroptosis in rat models with CPB-induced ALI. (a) Pyroptosis-related proteins in the lung tissues of rats with CPB-induced acute lung injury, including NLRP3, ASC, and caspase 1 were examined by immunofluorescence analysis. (b) Quantitative analysis of NLRP3, ASC, and caspase 1 expression. Scare bar = 100 μm. Values are expressed as mean ± SD. ∗P < 0.05; ∗∗P < 0.01, vs. CPB group.

Application: WB    Species: Rat    Sample: lung tissues

Figure 7 XFZYD inhibited the IKBα/NF-κB signaling in rat models with CPB-induced ALI. (a) The mRNA expression levels of NLRP3, ASC, GSDMD, and caspase 1 in CPB-induced lung injury rats treated with XFZYD, Ac-YVAD-CMK, and Bay-11-7082 were measured via RT-qPCR. (b and c) western blots were performed to determine NLRP3, ASC, Caspase-1 p20, Pro-GSDMD, GSDMD p30, IL-18, IL-1β p-P65, P65, p-IKBα, and IKBα levels in lung tissues of rats with CPB-induced acute lung injury. β-actin was used as a loading control for the blots. All the data were presented as the means ± SD. from independent experiments performed in triplicate. ∗P < 0.05; ∗∗P < 0.01, vs. CPB group.

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