RAP1A Antibody - #DF6157
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(3)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6157, RRID:AB_2838124.
折りたたみ／展開
C21KG; G 22K; G-22K; GTP binding protein smg p21A; GTP-binding protein smg p21A; KREV 1; KREV1; OTTHUMP00000013741; RAP 1A; RAP1; RAP1A; RAP1A member of RAS oncogene family; RAP1A_HUMAN; Ras related protein Krev 1; Ras related protein Rap 1A; RAS related protein RAP1A; Ras-related protein Krev-1; Ras-related protein Rap-1A; SMGP21;
免疫原
- P62834 RAP1A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDCQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDERVVGKEQGQNLARQWCNCAFLESSAKSKINVNEIFYDLVRQINRKTPVEKKKPKKKSCLLL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P62834 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K5 | Ubiquitination | Uniprot | |
| S11 | Phosphorylation | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| Y32 | Phosphorylation | Uniprot | |
| S39 | Phosphorylation | Uniprot | |
| Y40 | Phosphorylation | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| K128 | Ubiquitination | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| Y159 | Phosphorylation | Uniprot | |
| S180 | Phosphorylation | P17612 (PRKACA) | Uniprot |
研究背景
Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes. Plays a role in nerve growth factor (NGF)-induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions.
Cell membrane>Lipid-anchor. Cytoplasm. Cytoplasm>Perinuclear region. Cell junction. Early endosome.
Note: Recruited from early endosome to late endosome compartment after nerve growth factor (NGF) stimulation. Localized with RAPGEF2 at cell-cell junctions (By similarity). Colocalized with RAPGEF2 in the perinuclear region.
Found in a complex, at least composed of ITGB1BP1, KRIT1 and RAP1A. Interacts (active GTP-bound form preferentially) with KRIT1 (via C-terminus FERM domain); the interaction does not induce the opening conformation of KRIT1. In its GTP-bound form interacts with PLCE1 and RADIL. Interacts with SGSM1, SGSM2 and SGSM3. Interacts (via GTP-bound active form) with RAPGEF2 (via Ras-associating domain).
Belongs to the small GTPase superfamily. Ras family.
研究領域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Immune system > Platelet activation. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
· Organismal Systems > Nervous system > Long-term potentiation.
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
· Organismal Systems > Digestive system > Pancreatic secretion.
参考文献
Application: WB Species: Rat Sample:
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