BIRC2 Antibody - #DF6167

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製品: BIRC2 Antibody
カタログ: DF6167
タンパク質の説明: Rabbit polyclonal antibody to BIRC2
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IHC
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Sheep, Rabbit, Dog
分子量: 70kDa; 70kD(Calculated).
ユニプロット: Q13490
RRID: AB_2838134

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:400
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(90%), Horse(90%), Sheep(90%), Rabbit(90%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
BIRC2 Antibody detects endogenous levels of total BIRC2.
RRID:
AB_2838134
引用形式: Affinity Biosciences Cat# DF6167, RRID:AB_2838134.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

API 1; API1; Apoptosis inhibitor 1; Baculoviral IAP repeat containing 2; Baculoviral IAP repeat containing protein 2; Baculoviral IAP repeat-containing protein 2; BIRC 2; BIRC2; BIRC2_HUMAN; C IAP1; C-IAP1; cIAP 1; cIAP1; HIAP 2; HIAP-2; HIAP2; IAP 2; IAP homolog B; IAP-2; IAP2; Inhibitor of apoptosis protein 2; MIHB; NFR2 TRAF signalling complex protein; RING finger protein 48; RNF 48; RNF48; TNFR2 TRAF signaling complex protein 2; TNFR2-TRAF-signaling complex protein 2;

免疫原

免疫原:

A synthesized peptide derived from human BIRC2, corresponding to a region within C-terminal amino acids.

Uniprot:

Q13490(BIRC2_HUMAN) >>Visit HPA database.

遺伝子(ID):
BIRC2(329)
発現特異性:
Q13490 BIRC2_HUMAN:

Present in many fetal and adult tissues. Mainly expressed in adult skeletal muscle, thymus, testis, ovary, and pancreas, low or absent in brain and peripheral blood leukocytes.

タンパク質の説明:
The inhibitor of apoptosis protein (IAP) family consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain (1,2). Human members of this family include c-IAP1, c-IAP2, XIAP, survivin, livin, and NAIP. Overexpression of IAP family members, particularly survivin and livin, in cancer cell lines and primary tumors suggests an important role for these proteins in cancer progression (3-5). In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7, and caspase-9 (5,6). In addition, binding of IAP family members to the mitochondrial protein Smac blocks their interaction with caspase-9, thereby allowing the processing and activation of the caspase (2).
タンパク質配列:
MHKTASQRLFPGPSYQNIKSIMEDSTILSDWTNSNKQKMKYDFSCELYRMSTYSTFPAGVPVSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKLGDSPIQKHKQLYPSCSFIQNLVSASLGSTSKNTSPMRNSFAHSLSPTLEHSSLFSGSYSSLSPNPLNSRAVEDISSSRTNPYSYAMSTEEARFLTYHMWPLTFLSPSELARAGFYYIGPGDRVACFACGGKLSNWEPKDDAMSEHRRHFPNCPFLENSLETLRFSISNLSMQTHAARMRTFMYWPSSVPVQPEQLASAGFYYVGRNDDVKCFCCDGGLRCWESGDDPWVEHAKWFPRCEFLIRMKGQEFVDEIQGRYPHLLEQLLSTSDTTGEENADPPIIHFGPGESSSEDAVMMNTPVVKSALEMGFNRDLVKQTVQSKILTTGENYKTVNDIVSALLNAEDEKREEEKEKQAEEMASDDLSLIRKNRMALFQQLTCVLPILDNLLKANVINKQEHDIIKQKTQIPLQARELIDTILVKGNAAANIFKNCLKEIDSTLYKNLFVDKNMKYIPTEDVSGLSLEEQLRRLQEERTCKVCMDKEVSVVFIPCGHLVVCQECAPSLRKCPICRGIIKGTVRTFLS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Horse
90
Bovine
90
Sheep
90
Rabbit
90
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling, and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, TRAF2, DIABLO/SMAC, MAP3K14/NIK, MAP3K5/ASK1, IKBKG/NEMO, IKBKE and MXD1/MAD1. Can also function as an E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Can stimulate the transcriptional activity of E2F1. Plays a role in the modulation of the cell cycle.

PTMs:

Auto-ubiquitinated and degraded by the proteasome in apoptotic cells.

Upon stimulation of death receptors, including TNFRSF10B, recruited to receptors and cleaved by caspases. Proteolytic fragments remain associated with the receptors. This cleavage presumably inactivates the protein.

細胞の位置付け:

Cytoplasm. Nucleus.
Note: Agents that induce either the extrinsic or intrinsic apoptotic pathways promote its redistribution from the nuclear compartment to the cytoplasmic compartment. Associated with the midbody in telophase cells, and found diffusely in the nucleus of interphase cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Present in many fetal and adult tissues. Mainly expressed in adult skeletal muscle, thymus, testis, ovary, and pancreas, low or absent in brain and peripheral blood leukocytes.

タンパク質ファミリー:

The BIR domains mediate nuclear localization.

The CARD domain is necessary to stabilize the protein and inhibit the activation of E3 ubiquitin-protein ligase activity of BIRC2/c-IAP1 by preventing RING domain dimerization and E2 ubiquitin donor binding and activation.

Belongs to the IAP family.

研究領域

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species.   (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

参考文献

1). TRAIL‐Armed ER Nanosomes Induce Drastically Enhanced Apoptosis in Resistant Tumor in Combination with the Antagonist of IAPs (AZD5582). Advanced Healthcare Materials, 2021 (PubMed: 33963815) [IF=10.0]
2). HIF-1 α Is Associated with Resistance to Hypoxia-Induced Apoptosis in Ameloblastoma. International journal of dentistry, 2021 (PubMed: 34987583) [IF=1.9]

Application: IHC    Species: Human    Sample: Ameloblastoma

Figure 2 Immunomarking for HIF-1α, IAP-2, GLUT1, and Bcl-2 in AMB and FP. HIF-1α staining in AMB (a) was nuclear in the solid tumour area (arrowheads) and cytoplasmic and nuclear in cells near the cystic areas of the epithelial islets (arrow and cystic lumen, ∗). IAP-2 labelling on AMB (c) was predominant in the cytoplasm and nucleus (arrow) of the cells of the epithelial islands. Marking is also detected in selected cells from the basal and central layers (arrowhead and arrow, resp.). GLUT1 immunostaining in AMB (e) is predominant in the cytoplasm of the cells of the basal and central layer of the epithelial islands (arrowhead and arrow, resp.). Bcl-2 immunostaining in AMB (g) is predominant in the cytoplasm of epithelial island cells (arrow). Marking is also detected in selected cells from the basal and central layers (arrowhead and arrow, resp.). The FP shows a weaker labelling of the proteins IAP-2, GLUT1, and Bcl-2 compared with AMB (b, d, f, h). Scale 100 and 20 μm. Comparison of immunoexpression of the HIF-1α, IAP-2, GLUT1 and Bcl-2, and HIF-1α in the ameloblastoma (a) and the follicle dental (f). ∗∗∗p < 0.001 (m).
3). Wnt/PCP-YAP-BIRC2 axis maintains cartilage stem/progenitor cell homeostasis in osteoarthritis. Research Square, 2022

Application: WB    Species: Human    Sample: CSPCs

Figure 7. Transcriptomic analysis. a KEGG and GO enrichment analysis of DEGs. b The detailed DEGs that enriched in cell cycle, cellular senescence, autophagy, apoptosis, Hippo signaling pathway, and TNF signaling pathways. c The direct downstream target genes of YAP. d The protein level of Birc2, Snai2, Zenb2, and Ccnd2 in CSPCs.

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