製品: ARF6 Antibody
カタログ: DF6170
タンパク質の説明: Rabbit polyclonal antibody to ARF6
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 20kDa; 20kD(Calculated).
ユニプロット: P62330
RRID: AB_2838137

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
ARF6 Antibody detects endogenous levels of total ARF6.
RRID:
AB_2838137
引用形式: Affinity Biosciences Cat# DF6170, RRID:AB_2838137.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

antibody;ADP ribosylation factor 6; ADP ribosylation factor protein 6; ADP-ribosylation factor 6; ARF6; ARF6_HUMAN; DKFZp564M0264; Small GTP binding protein; Small GTPase;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P62330 ARF6_HUMAN:

Ubiquitous, with higher levels in heart, substantia nigra, and kidney.

タンパク質の説明:
ADP-ribosylation factor (Arf) proteins are low molecular weight GTP binding proteins that belong to the Ras GTPase superfamily (1). Arf proteins are grouped into three distinct classes based on amino acid sequence and structural similarity, with Arf6 as the single class III protein to date. Arf6 is localized mainly to the plasma membrane and endosomes (1,2). This small GTPase interacts with PIP5K, PLD and Rac1, proteins important in lipid metabolism and actin regulation. Arf6 function depends upon its cycling between GDP- and GTP-bound states, which is regulated by associated GAP and GEF factors (3,4). Plasma membrane-associated Arf6 appears to play several functions during the many steps of membrane trafficking, including regulating membrane receptor internalization in both clathrin-dependent and independent pathways, endosomal recycling, and proximal actin reorganization and remodeling (5,6).
タンパク質配列:
MGKVLSKIFGNKEMRILMLGLDAAGKTTILYKLKLGQSVTTIPTVGFNVETVTYKNVKFNVWDVGGQDKIRPLWRHYYTGTQGLIFVVDCADRDRIDEARQELHRIINDREMRDAIILIFANKQDLPDAMKPHEIQEKLGLTRIRDRNWYVQPSCATSGDGLYEGLTWLTSNYKS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P62330 基板として

Site PTM Type Enzyme
G2 Myristoylation
K7 Ubiquitination
K12 Ubiquitination
Y31 Phosphorylation
K32 Acetylation
K32 Ubiquitination
K34 Ubiquitination
T41 Phosphorylation
K55 Ubiquitination
K58 Ubiquitination
K69 Sumoylation
K69 Ubiquitination
C90 S-Nitrosylation
K131 Ubiquitination
K138 Ubiquitination
Y150 Phosphorylation
Y163 Phosphorylation
T167 Phosphorylation
K174 Ubiquitination

研究背景

機能:

GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling. Required for normal completion of mitotic cytokinesis (By similarity). Plays a role in the reorganization of the actin cytoskeleton and the formation of stress fibers (By similarity). Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension. Plays an important role in membrane trafficking, during junctional remodeling and epithelial polarization. Regulates surface levels of adherens junction proteins such as CDH1 (By similarity). Required for NTRK1 sorting to the recycling pathway from early endosomes (By similarity).

(Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.

細胞の位置付け:

Cytoplasm>Cytosol. Cell membrane>Lipid-anchor. Endosome membrane>Lipid-anchor. Recycling endosome membrane>Lipid-anchor. Cell projection>Filopodium membrane>Lipid-anchor. Cell projection>Ruffle. Cleavage furrow. Midbody>Midbody ring. Early endosome membrane>Lipid-anchor. Golgi apparatus>trans-Golgi network membrane>Lipid-anchor.
Note: Distributed uniformly on the plasma membrane, as well as throughout the cytoplasm during metaphase. Subsequently concentrated at patches in the equatorial region at the onset of cytokinesis, and becomes distributed in the equatorial region concurrent with cleavage furrow ingression. In late stages of cytokinesis, concentrates at the midbody ring/Flemming body (PubMed:23603394). Recruitment to the midbody ring requires both activation by PSD/EFA6A and interaction with KIF23/MKLP1 (By similarity). After abscission of the intercellular bridge, incorporated into one of the daughter cells as a midbody remnant and localizes to punctate structures beneath the plasma membrane (PubMed:23603394). Recruited to the cell membrane in association with CYTH2 and ARL4C. Colocalizes with DAB2IP at the plasma membrane and endocytic vesicles (By similarity). Myristoylation is required for proper localization to membranes (PubMed:7589240).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitous, with higher levels in heart, substantia nigra, and kidney.

サブユニット構造:

Interacts (when activated) with GGA1, GGA2 and GGA3; the interaction is required for proper subcellular location of GGA1, GGA2 and GGA3. Interacts with PIP5K1C. Interacts with USP6 (via Rab-GAP TBC domain). Interacts with RAB11FIP3 and RAB11FIP4. Interacts with HERC1. Interacts with ARHGAP21. Interacts with ASAP3; the interaction is stabilized by calcium ions. Interacts with NCS1/FREQ at the plasma membrane. Interacts with TBC1D24. Interacts with ECPAS. Interacts with MICALL1. Interacts with SPAG9 homodimers, forming heterotetramers. Interacts with CYTH3. Interacts with ASAP2 (By similarity). Interacts with UACA (By similarity). Interacts with KIF23, forming heterodimers and heterotetramers (By similarity). Interacts with C9orf72 (By similarity).

(Microbial infection) Interacts with the V.cholerae enterotoxin subunit A1; this causes a conformation change so that the toxin can bind NAD and catalyze the ADP-ribosylation of Gs alpha.

(Microbial infection) Interacts with EspG from enteropathogenic E.coli.

(Microbial infection) Identified in a complex with RAB1A and EspG from enteropathogenic E.coli.

タンパク質ファミリー:

Belongs to the small GTPase superfamily. Arf family.

研究領域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

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