TPMT Antibody - #DF6183
| 製品: | TPMT Antibody |
| カタログ: | DF6183 |
| タンパク質の説明: | Rabbit polyclonal antibody to TPMT |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Bovine, Horse, Rabbit |
| 分子量: | 28kDa, 35 kDa; 28kD(Calculated). |
| ユニプロット: | P51580 |
| RRID: | AB_2838149 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6183, RRID:AB_2838149.
折りたたみ／展開
HGNC:12014; S adenosyl L methionine thiopurine S methyltransferase; Thiopurine methyltransferase; Thiopurine S methyltransferase; Thiopurine S-methyltransferase; TPMT; TPMT_HUMAN;
免疫原
- P51580 TPMT_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDGTRTSLDIEEYSDTEVQKNQVLTLEEWQDKWVNGKTAFHQEQGHQLLKKHLDTFLKGKSGLRVFFPLCGKAVEMKWFADRGHSVVGVEISELGIQEFFTEQNLSYSEEPITEIPGTKVFKSSSGNISLYCCSIFDLPRTNIGKFDMIWDRGALVAINPGDRKCYADTMFSLLGKKFQYLLCVLSYDPTKHPGPPFYVPHAEIERLFGKICNIRCLEKVDAFEERHKSWGIDCLFEKLYLLTEK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P51580 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T6 | Phosphorylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| Y13 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| T16 | Phosphorylation | Uniprot | |
| T25 | Phosphorylation | Uniprot | |
| K32 | Acetylation | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| K37 | Ubiquitination | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| K58 | Acetylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| K77 | Acetylation | Uniprot | |
| Y166 | Phosphorylation | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| K210 | Ubiquitination | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| S229 | Phosphorylation | Uniprot |
研究背景
Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine (also called mercaptopurine, 6-MP or its brand name Purinethol) and 6-thioguanine (also called tioguanine or 6-TG) using S-adenosyl-L-methionine as the methyl donor. TPMT activity modulates the cytotoxic effects of thiopurine prodrugs. A natural substrate for this enzyme has yet to be identified.
Cytoplasm.
Monomer.
Belongs to the class I-like SAM-binding methyltransferase superfamily. TPMT family.
研究領域
· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.
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