ADA Antibody - #DF6184
| 製品: | ADA Antibody |
| カタログ: | DF6184 |
| タンパク質の説明: | Rabbit polyclonal antibody to ADA |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 41kDa; 41kD(Calculated). |
| ユニプロット: | P00813 |
| RRID: | AB_2838150 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6184, RRID:AB_2838150.
折りたたみ／展開
ada; ADA_HUMAN; ADA1; Adenosine aminohydrolase; Adenosine deaminase;
免疫原
Found in all tissues, occurs in large amounts in T-lymphocytes (PubMed:20959412). Expressed at the time of weaning in gastrointestinal tissues.
- P00813 ADA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAQTPAFDKPKVELHVHLDGSIKPETILYYGRRRGIALPANTAEGLLNVIGMDKPLTLPDFLAKFDYYMPAIAGCREAIKRIAYEFVEMKAKEGVVYVEVRYSPHLLANSKVEPIPWNQAEGDLTPDEVVALVGQGLQEGERDFGVKARSILCCMRHQPNWSPKVVELCKKYQQQTVVAIDLAGDETIPGSSLLPGHVQAYQEAVKSGIHRTVHAGEVGSAEVVKEAVDILKTERLGHGYHTLEDQALYNRLRQENMHFEICPWSSYLTGAWKPDTEHAVIRLKNDQANYSLNTDDPLIFKSTLDTDYQMTKRDMGFTEEEFKRLNINAAKSSFLPEDEKRELLDLLYKAYGMPPSASAGQNL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P00813 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T4 | Phosphorylation | Uniprot | |
| K9 | Acetylation | Uniprot | |
| K9 | Ubiquitination | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| T26 | Phosphorylation | Uniprot | |
| Y29 | Phosphorylation | Uniprot | |
| Y30 | Phosphorylation | Uniprot | |
| K54 | Acetylation | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| Y67 | Phosphorylation | Uniprot | |
| Y84 | Phosphorylation | Uniprot | |
| K90 | Ubiquitination | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K111 | Ubiquitination | Uniprot | |
| K147 | Ubiquitination | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| K164 | Acetylation | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| K170 | Acetylation | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| K225 | Ubiquitination | Uniprot | |
| K232 | Acetylation | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| Y240 | Phosphorylation | Uniprot | |
| Y249 | Phosphorylation | Uniprot | |
| K273 | Ubiquitination | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| Y290 | Phosphorylation | Uniprot | |
| S291 | Phosphorylation | Uniprot | |
| K301 | Ubiquitination | Uniprot | |
| Y308 | Phosphorylation | Uniprot | |
| K312 | Acetylation | Uniprot | |
| K312 | Ubiquitination | Uniprot | |
| K323 | Acetylation | Uniprot | |
| K323 | Ubiquitination | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| K340 | Ubiquitination | Uniprot | |
| Y348 | Phosphorylation | Uniprot | |
| Y351 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot |
研究背景
Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion (By similarity). Enhances CD4+ T-cell differentiation and proliferation. Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change. Stimulates plasminogen activation. Plays a role in male fertility. Plays a protective role in early postimplantation embryonic development (By similarity).
Cell membrane>Peripheral membrane protein>Extracellular side. Cell junction. Cytoplasmic vesicle lumen. Cytoplasm. Lysosome.
Note: Colocalized with DPP4 at the cell surface.
Found in all tissues, occurs in large amounts in T-lymphocytes. Expressed at the time of weaning in gastrointestinal tissues.
Interacts with DPP4 (via extracellular domain). Interacts with PLG (via Kringle 4 domain); the interaction stimulates PLG activation when in complex with DPP4.
Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.
研究領域
· Human Diseases > Immune diseases > Primary immunodeficiency.
· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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