PSMD4 Antibody - #DF6206
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6206, RRID:AB_2838172.
折りたたみ／展開
26S protease subunit S5a; 26S proteasome non ATPase regulatory subunit 4; 26S proteasome non-ATPase regulatory subunit 4; 26S proteasome regulatory subunit rpn10; 26S proteasome regulatory subunit S5A; AF 1; AF; AF1; Angiocidin; Antisecretory factor 1; ASF; DS5a; MCB 1; MCB1; Multiubiquitin chain binding protein; Multiubiquitin chain-binding protein; OTTHUMP00000059963; Prosome macropain; Proteasome (prosome macropain) 26S subunit non ATPase 4; Proteasome 26S non ATPase subunit 4; Proteasome 26S subunit non ATPase 4; PSMD 4; Psmd4; PSMD4_HUMAN; pUB R5; pUBR5; Rpn 10; Rpn10; RPN10 homolog; S5A; S5a/antisecretory factor protein;
免疫原
- P55036 PSMD4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVLESTMVCVDNSEYMRNGDFLPTRLQAQQDAVNIVCHSKTRSNPENNVGLITLANDCEVLTTLTPDTGRILSKLHTVQPKGKITFCTGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDNEKDLVKLAKRLKKEKVNVDIINFGEEEVNTEKLTAFVNTLNGKDGTGSHLVTVPPGPSLADALISSPILAGEGGAMLGLGASDFEFGVDPSADPELALALRVSMEEQRQRQEEEARRAAAASAAEAGIATTGTEDSDDALLKMTISQQEFGRTGLPDLSSMTEEEQIAYAMQMSLQGAEFGQAESADIDASSAMDTSEPAKEEDDYDVMQDPEFLQSVLENLPGVDPNNEAIRNAMGSLASQATKDGKKDKKEEDKK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P55036 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| C9 | S-Nitrosylation | Uniprot | |
| C37 | S-Nitrosylation | Uniprot | |
| K40 | Ubiquitination | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| C87 | S-Nitrosylation | Uniprot | |
| K98 | Ubiquitination | Uniprot | |
| S115 | Phosphorylation | Uniprot | |
| K122 | Acetylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K126 | Acetylation | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| K133 | Ubiquitination | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| T250 | Phosphorylation | Uniprot | |
| T253 | Phosphorylation | Uniprot | |
| S256 | Phosphorylation | Uniprot | |
| K262 | Ubiquitination | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| Y326 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| S361 | Phosphorylation | Uniprot | |
| K365 | Acetylation | Uniprot | |
| K365 | Ubiquitination | Uniprot | |
| K368 | Acetylation | Uniprot | |
| K369 | Ubiquitination | Uniprot |
研究背景
Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMD4 acts as an ubiquitin receptor subunit through ubiquitin-interacting motifs and selects ubiquitin-conjugates for destruction. Displays a preferred selectivity for longer polyubiquitin chains.
Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases and few additional components including PSMD4. Interacts with NUB1. Interacts with SQSTM1. Interacts with UBQLN4. Interacts with UBE3A. Interacts with UBQLN1 (via ubiquitin-like domain). Interacts with DDI2.
The 2 UIM motifs are involved in the binding to a multi-ubiquitin chain in a cooperative way.
Belongs to the proteasome subunit S5A family.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Proteasome.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.