Alkaline Phosphatase Antibody - #DF6225

Figure 7. The dynamic HA-ADA hydrogel enhances the osteogenic differentiation. D) Representative images and E) quantified intensity (n = 20) of ALP (green) immunostaining of the hMSCs in the hydrogels.

Figure 3 circRNA422 interfering inhibited osteogenic differentiation of BMSCs (A) ALP staining to evaluate the effect of circRNA422− on the ALP activity of BMSCs on osteogenic days 3 and 7. (B) Protein levels of OCN to evaluate the effect of circRNA422− on mineralization of BMSCs on osteogenic days 14 and 21. (C) Quantitative real-time PCR analysis showed that the osteogenic mRNA expressions levels of SP7, LRP5, ALP, OCN, BSP, and OPN were downregulated by circRNA422− compared with Con313 on osteogenic days 3 and 7 (n = 3, ∗p < 0.05). (D) WB analysis showed that the osteogenic protein expressions levels of ALP, LRP5, and SP7 were reduced by circRNA422− compared with Con313 on osteogenic days 3 and 7 (n = 3, ∗p < 0.05).

Fig. 5 |ADSC-Exos improved the healing of tendon injury. a, h The H&E staining of tendon injury at week 2 and week 4. b–f, i–m The expression of TNMD, collagen I, SCXA, ALP, and Runx2 were detected by immunohistochemistry assay at week 2 (n = 6) and week 4 (n = 6).

Figure 2 Constitutive activation of FGFR2 (p.Cys342Arg) enhanced osteogenesis via Erk1/2 MAPK signaling pathway: (A) Schematic representation of the relative linear location in which the FGFR2 mutation is identified as illustrated by the large red arrow shown in the context of the protein structure. (B) CCK-8 assay was carried out to assess cell proliferation. Proliferation of MC3T3-E1 cells was more active in the MT group. (C) Relative expressions of osteogenic marker measured by qPCR. The expressions of Alp, ColIα2, Runx2, Opn and Ocn mRNA in differentiated MC3T3-e1 were remarkably increased in the MT group. Western blot analysis showed that the level of ALP, COLI and RUNX2 were also increased in the MT group. (D) ALP staining, alizarin red staining and quantitative tests. ALP staining showed increased crystal violet-staining cells in the MT group compared with the WT group. Quantitative experiment demonstrated that ALP activity is more active in the MT group. Alizarin red staining and quantitative test showed there were more mineralized nodules and mineral content in the MT group than in the WT group. (E) Western blot analysis demonstrated that the levels of p-FGFR2 and p-Erk1/2 were increased in the MT group. There was no significant change in the expression of key proteins in other downstream pathways. The western blot results of FGF/FGFR2-Erk1/2 was circled by the red frame. p values were significant at * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.