PDIA3 Antibody - #DF6230

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製品: PDIA3 Antibody
カタログ: DF6230
タンパク質の説明: Rabbit polyclonal antibody to PDIA3
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 57kDa; 57kD(Calculated).
ユニプロット: P30101
RRID: AB_2838196

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(83%)
クローナリティ:
Polyclonal
特異性:
PDIA3 Antibody detects endogenous levels of total PDIA3.
RRID:
AB_2838196
引用形式: Affinity Biosciences Cat# DF6230, RRID:AB_2838196.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

58 kDa glucose regulated protein; 58 kDa glucose-regulated protein; 58 kDa microsomal protein; Disulfide isomerase ER 60; Disulfide isomerase ER-60; Endoplasmic reticulum resident protein 57; Endoplasmic reticulum resident protein 60; ER p57; ER protein 57; ER protein 60; ERp 57; ERp57; ERp60; ERp61; Glucose Regulated Protein 58 Kd; GRP 57; GRP 58; GRP57; HsT17083; p58; PDIA 3; PDIA3; PDIA3_HUMAN; Phospholipase C alpha; PI PLC; Protein disulfide isomerase A3; Protein disulfide isomerase family A member 3; Protein disulfide-isomerase A3;

免疫原

免疫原:
Uniprot:

P30101(PDIA3_HUMAN) >>Visit HPA database.

遺伝子(ID):
PDIA3(2923)
発現特異性:
P30101 PDIA3_HUMAN:

Detected in the flagellum and head region of spermatozoa (at protein level).

タンパク質の説明:
Secretory proteins translocate into the endoplasmic reticulum (ER) after their synthesis where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Disulfide isomerase (PDI) has two thioredoxin homology domains and catalyzes the formation and isomerization of these disulfide bonds (2). Other ER resident proteins that possess the thioredoxin homology domains, including endoplasmic reticulum stress protein 57 (ERp57), constitute the PDI family (2). ERp57 interacts with calnexin and calreticulin (3) and is suggested to play a role in the isomerization of disulfide bonds on certain glycoproteins (3).
タンパク質配列:
MRLRRLALFPGVALLLAAARLAAASDVLELTDDNFESRISDTGSAGLMLVEFFAPWCGHCKRLAPEYEAAATRLKGIVPLAKVDCTANTNTCNKYGVSGYPTLKIFRDGEEAGAYDGPRTADGIVSHLKKQAGPASVPLRTEEEFKKFISDKDASIVGFFDDSFSEAHSEFLKAASNLRDNYRFAHTNVESLVNEYDDNGEGIILFRPSHLTNKFEDKTVAYTEQKMTSGKIKKFIQENIFGICPHMTEDNKDLIQGKDLLIAYYDVDYEKNAKGSNYWRNRVMMVAKKFLDAGHKLNFAVASRKTFSHELSDFGLESTAGEIPVVAIRTAKGEKFVMQEEFSRDGKALERFLQDYFDGNLKRYLKSEPIPESNDGPVKVVVAENFDEIVNNENKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVPSPYEVRGFPTIYFSPANKKLNPKKYEGGRELSDFISYLQREATNPPVIQEEKPKKKKKAQEDL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Rabbit
100
Bovine
92
Sheep
92
Chicken
83
Zebrafish
75
Xenopus
67
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30101 基板として

Site PTM Type Enzyme
S37 Phosphorylation
K61 Methylation
Y67 Phosphorylation
R73 Methylation
K75 Ubiquitination
K82 Ubiquitination
C85 S-Nitrosylation
T86 Phosphorylation
C92 S-Nitrosylation
K94 Acetylation
K94 Ubiquitination
S98 Phosphorylation
Y100 Phosphorylation
T102 O-Glycosylation
T102 Phosphorylation
K104 Acetylation
K104 Ubiquitination
Y115 Phosphorylation
R119 Methylation
S126 Phosphorylation
S136 Phosphorylation
R140 Methylation
T141 Phosphorylation
S163 Phosphorylation
S165 Phosphorylation
K173 Ubiquitination
S176 Phosphorylation
R179 Methylation
Y196 Phosphorylation
K214 Ubiquitination
T223 Phosphorylation
K226 Acetylation
K226 Ubiquitination
C244 S-Nitrosylation
Y269 Phosphorylation
K289 Ubiquitination
K296 Ubiquitination
S303 Phosphorylation
K305 Ubiquitination
S312 Phosphorylation
S318 Phosphorylation
T319 Phosphorylation
R329 Methylation
K335 Ubiquitination
S343 Phosphorylation
Y356 Phosphorylation
K362 Acetylation
K362 Ubiquitination
K366 Ubiquitination
S367 Phosphorylation
S373 Phosphorylation
Y402 Phosphorylation
S424 Phosphorylation
K425 Ubiquitination
K433 Ubiquitination
S443 Phosphorylation
Y445 Phosphorylation
R448 Methylation
T452 O-Glycosylation
T452 Phosphorylation
Y454 Phosphorylation
S456 O-Glycosylation
S456 Phosphorylation P06493 (CDK1)
S474 Phosphorylation
S478 Phosphorylation
Y479 Phosphorylation
T485 O-Glycosylation

研究背景

細胞の位置付け:

Endoplasmic reticulum. Endoplasmic reticulum lumen. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Detected in the flagellum and head region of spermatozoa (at protein level).

サブユニット構造:

Subunit of the TAP complex, also known as the peptide loading complex (PLC). Can form disulfide-linked heterodimers with TAPBP. Interacts with ERP27 and CANX. Interacts with SERPINA2 and with the S and Z variants of SERPINA1. Interacts with ATP2A2 (By similarity).

タンパク質ファミリー:

Belongs to the protein disulfide isomerase family.

研究領域

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Organismal Systems > Immune system > Antigen processing and presentation.   (View pathway)

参考文献

1). Novel functional mutation of the PDIA3 gene affects milk composition traits in Chinese Holstein cattle. JOURNAL OF DAIRY SCIENCE, 2022 (PubMed: 35379459) [IF=3.5]

Application: IF/ICC    Species: cow    Sample:

Figure 4. Expression and subcellular localization of PDIA3. (A) mRNA expression profile of PDIA3 in different tissues of dairy cows by quantitative real-time PCR method; (B) subcellular localization of PDIA3 in bovine mammary alveolar cells observed by immunofluorescence assay. PDIA3 (green); endoplasmic reticulum (ER) signals (red); nucleus stained with 4',6-diamidino-2-phenylindole dihydrochloride (DAPI, blue). Scale bar represents 10 μm.

Application: WB    Species: cow    Sample:

Figure 5. Detection of the efficiency of PDIA3 overexpression and knockdown in bovine mammary alveolar cells. (A) Map of PDIA3 overexpression vector (left), and agarose gel electrophoresis of pCDNA3.1- PDIA3 vector digested with restriction endonucleases (right). M = DNA marker. Lane 1 (L1) shows 2 bands of 2,248 bp and 4,671 bp with Mlu I and Xho I digestion; lane 2 (L2) shows the pCDNA3.1- PDIA3 plasmid. (B) Cells were transfected for 48 h with pCDNA3.1- PDIA3 overexpression vector (OE), pCDNA3.1 empty vector (EV), PDIA3 siRNA (KD), or negative control siRNA (NC), and the relative mRNA levels of PDIA3 were analyzed by quantitative real-time PCR. (C) Protein levels of PDIA3 were determined by western blot analysis, and quantitative results show fold increases in PDIA3/GAPDH ratios. **P < 0.01, compared with control group; ns = not significant. Error bars represent SEM. siRNA = small interfering RNA.

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