Prealbumin Antibody - #DF6256

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製品: Prealbumin Antibody
カタログ: DF6256
タンパク質の説明: Rabbit polyclonal antibody to Prealbumin
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 16kDa; 16kD(Calculated).
ユニプロット: P02766
RRID: AB_2838222

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(86%), Dog(100%), Chicken(86%)
クローナリティ:
Polyclonal
特異性:
Prealbumin Antibody detects endogenous levels of total Prealbumin.
RRID:
AB_2838222
引用形式: Affinity Biosciences Cat# DF6256, RRID:AB_2838222.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Amyloid polyneuropathy; Amyloidosis I; ATTR; Carpal tunnel syndrome 1; CTS; CTS1; Dysprealbuminemic euthyroidal hyperthyroxinemia; Dystransthyretinemic hyperthyroxinemia; Epididymis luminal protein 111; HEL111; HsT2651; PALB; Prealbumin amyloidosis type I; Prealbumin; Prealbumin Thyroxine-binding; Senile systemic amyloidosis; TBPA; Thyroxine binding prealbumin; Transthyretin; TTHY_HUMAN; TTR; TTR protein;

免疫原

免疫原:
Uniprot:

P02766(TTHY_HUMAN) >>Visit HPA database.

遺伝子(ID):
TTR(7276)
発現特異性:
P02766 TTHY_HUMAN:

Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.

タンパク質の説明:
This gene encodes transthyretin, one of the three prealbumins including alpha-1-antitrypsin, transthyretin and orosomucoid. Transthyretin is a carrier protein; it transports thyroid hormones in the plasma and cerebrospinal fluid, and also transports retinol (vitamin A) in the plasma. The protein consists of a tetramer of identical subunits. More than 80 different mutations in this gene have been reported; most mutations are related to amyloid deposition, affecting predominantly peripheral nerve and/or the heart, and a small portion of the gene mutations is non-amyloidogenic. The diseases caused by mutations include amyloidotic polyneuropathy, euthyroid hyperthyroxinaemia, amyloidotic vitreous opacities, cardiomyopathy, oculoleptomeningeal amyloidosis, meningocerebrovascular amyloidosis, carpal tunnel syndrome, etc. [provided by RefSeq, Jan 2009]
タンパク質配列:
MASHRLLLLCLAGLVFVSEAGPTGTGESKCPLMVKVLDAVRGSPAINVAVHVFRKAADDTWEPFASGKTSESGELHGLTTEEEFVEGIYKVEIDTKSYWKALGISPFHEHAEVVFTANDSGPRRYTIAALLSPYSYSTTAVVTNPKE

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
86
Rabbit
86
Zebrafish
83
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P02766 基板として

Site PTM Type Enzyme
C10 S-Nitrosylation
T23 Phosphorylation
T25 Phosphorylation
S72 Phosphorylation
N118 N-Glycosylation
Y125 Phosphorylation
S137 Phosphorylation
T138 Phosphorylation

研究背景

機能:

Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.

PTMs:

Not glycosylated under normal conditions. Following unfolding, caused for example by variant AMYL-TTR 'Gly-38', the cryptic Asn-118 site is exposed and glycosylated by STT3B-containing OST complex, leading to its degradation by the ER-associated degradation (ERAD) pathway.

細胞の位置付け:

Secreted. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.

サブユニット構造:

Homotetramer. Dimer of dimers. In the homotetramer, subunits assemble around a central channel that can accommodate two ligand molecules. Interacts with RBP4.

タンパク質ファミリー:

Each monomer has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel.

Belongs to the transthyretin family.

研究領域

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

参考文献

1). Tetraiodothyroacetic acid and transthyretin silencing inhibit pro-metastatic effect of L-thyroxin in anoikis-resistant prostate cancer cells through regulation of MAPK/ERK pathway. EXPERIMENTAL CELL RESEARCH, 2016 (PubMed: 27569004) [IF=3.7]

Application: WB    Species: human    Sample: HepG2

(B) Western blotting indicated that expression of TTR gradually increased along with enhanced metastatic potential 23 in different prostate cancer cell lines (HepG2 cells were used as a positive control). (C) Western blotting indicated that together with TTR, expressions of XIAP, MMP-2 and VEGF were remarkably higher in anoikis-resistant PC-3 cells in contrast to that of parental cells

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