CRH Antibody - #DF6258

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製品: CRH Antibody
カタログ: DF6258
タンパク質の説明: Rabbit polyclonal antibody to CRH
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Sheep, Dog
分子量: 21kDa; 21kD(Calculated).
ユニプロット: P06850
RRID: AB_2838224

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(92%), Sheep(92%), Dog(92%)
クローナリティ:
Polyclonal
特異性:
CRH Antibody detects endogenous levels of total CRH.
RRID:
AB_2838224
引用形式: Affinity Biosciences Cat# DF6258, RRID:AB_2838224.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Corticoliberin; Corticotropin releasing factor; Corticotropin releasing hormone; CRF; Crh; CRH1;

免疫原

免疫原:
Uniprot:

P06850(CRF_HUMAN) >>Visit HPA database.

遺伝子(ID):
CRH(1392)
発現特異性:
P06850 CRF_HUMAN:

Produced by the hypothalamus and placenta.

タンパク質の説明:
Corticotropin-releasing hormone is secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Marked reduction in this protein has been observed in association with Alzheimer disease and autosomal recessive hypothalamic corticotropin deficiency has multiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of the hormone occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, this protein may act as a trigger for parturition. [provided by RefSeq, Apr 2010]
タンパク質配列:
MRLPLLVSAGVLLVALLPCPPCRALLSRGPVPGARQAPQHPQPLDFFQPPPQSEQPQQPQARPVLLRMGEEYFLRLGNLNKSPAAPLSPASSLLAGGSGSRPSPEQATANFFRVLLQQLLLPRRSLDSPAALAERGARNALGGHQEAPERERRSEEPPISLDLTFHLLREVLEMARAEQLAQQAHSNRKLMEIIGK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
92
Sheep
92
Dog
92
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P06850 基板として

Site PTM Type Enzyme
S125 Phosphorylation

研究背景

機能:

Hormone regulating the release of corticotropin from pituitary gland (By similarity). Induces NLRP6 in intestinal epithelial cells, hence may influence gut microbiota profile (By similarity).

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Produced by the hypothalamus and placenta.

サブユニット構造:

Interacts (via C-terminus) with CRFR1 (via N-terminal extracellular domain).

タンパク質ファミリー:

Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family.

研究領域

· Human Diseases > Substance dependence > Alcoholism.

· Organismal Systems > Nervous system > Long-term depression.

参考文献

1). Corticotropin-releasing factor induces inflammatory cytokines via the NLRP6-inflammatory cytokine axis in a murine model of irritable bowel syndrome. Journal of Digestive Diseases, 2019 (PubMed: 30663229) [IF=3.5]

Application: WB    Species: mouse    Sample: colonic

FIGURE 2 |Corticotropin-releasing factor (CRF) inhibition of NOD-like receptor pyrin domain-containing protein 6 (NLRP6) expression and Clodistrium butyricum (C. butyricum) restoration of CRF expression in (A-D) the control, irritable bowel syndrome (IBS) model and C. butyricumtreated groups (n = 6 per group) or (E,F) Caco-2 cells. (A) Western blot analysis and (B) gray scale values measured by Quantity One software of CRF expression in colonic tissues.

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