TNNT2 Antibody - #DF6261

(3)  
製品: TNNT2 Antibody
カタログ: DF6261
タンパク質の説明: Rabbit polyclonal antibody to TNNT2
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Sheep, Rabbit, Dog
分子量: 36kDa; 36kD(Calculated).
ユニプロット: P45379
RRID: AB_2838227

類似製品を見る>>

   サイズ 価格 在庫状況
 100ul $280 在庫あり
 200ul $350 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先
関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Horse(100%), Sheep(90%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
TNNT2 Antibody detects endogenous levels of total TNNT2.
RRID:
AB_2838227
引用形式: Affinity Biosciences Cat# DF6261, RRID:AB_2838227.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Cardiac muscle troponin T; Cardiomyopathy dilated 1D (autosomal dominant); Cardiomyopathy hypertrophic 2; CMD1D; CMH2; CMPD2; cTnT; LVNC6; MGC3889; OTTHUMP00000033864; OTTHUMP00000033865; OTTHUMP00000033866; OTTHUMP00000033867; OTTHUMP00000033870; OTTHUMP00000218095; RCM3; TNNT 2; TNNT2; TNNT2_HUMAN; TnTc; Troponin T cardiac muscle; Troponin T type 2 (cardiac); Troponin T type 2 cardiac; Troponin T, cardiac muscle; Troponin T2 cardiac;

免疫原

免疫原:
Uniprot:

P45379(TNNT2_HUMAN) >>Visit HPA database.

遺伝子(ID):
TNNT2(7139)
発現特異性:
P45379 TNNT2_HUMAN:

Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.

タンパク質の説明:
Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits: the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI) (1). In response to β-adrenergic stimulation of the heart, Ser23 and Ser24 of TnI (cardiac) are phosphorylated by PKA and PKC. This phosphorylation stimulates a conformational change of the regulatory domain of TnC, reduces the association between TnI and TnC, and decreases myofilament Ca2+ sensitivity by reducing the Ca2+ binding affinity of TnC (1-3). The tropomyosin binding subunit of the troponin complex TnT exists as different isoforms in slow skeletal muscle (ssTnT/TNNT1), fast skeletal muscle (fsTnT/TNNT3) and in cardiac muscle (cTnT/TNNT2). Each of these may also contain multiple alternatively spliced variants. Assays for measuring serum concentrations of cTnT, as well as cTnI, have been reported for analyzing cardiac injury.
タンパク質配列:
MSDIEEVVEEYEEEEQEEAAVEEEEDWREDEDEQEEAAEEDAEAEAETEETRAEEDEEEEEAKEAEDGPMEESKPKPRSFMPNLVPPKIPDGERVDFDDIHRKRMEKDLNELQALIEAHFENRKKEEEELVSLKDRIERRRAERAEQQRIRNEREKERQNRLAEERARREEEENRRKAEDEARKKKALSNMMHFGGYIQKQAQTERKSGKRQTEREKKKKILAERRKVLAIDHLNEDQLREKAKELWQSIYNLEAEKFDLQEKFKQQKYEINVLRNRINDNQKVSKTRGKAKVTGRWK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Rabbit
100
Sheep
90
Chicken
67
Xenopus
56
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P45379 基板として

Site PTM Type Enzyme
S2 Phosphorylation
S189 Phosphorylation P17252 (PRKCA)
Y197 Phosphorylation
T204 Phosphorylation P17252 (PRKCA) , Q99683 (MAP3K5)
S208 Phosphorylation Q99683 (MAP3K5)
T213 Phosphorylation P04049 (RAF1)
S249 Phosphorylation
Y251 Phosphorylation
K290 Acetylation
K292 Acetylation
T294 Phosphorylation

研究背景

機能:

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

PTMs:

Phosphorylation at Thr-213 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity.

組織特異性:

Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.

タンパク質ファミリー:

Belongs to the troponin T family.

研究領域

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Organismal Systems > Circulatory system > Cardiac muscle contraction.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.