FBL Antibody - #DF6270
| 製品: | FBL Antibody |
| カタログ: | DF6270 |
| タンパク質の説明: | Rabbit polyclonal antibody to FBL |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 34kDa; 34kD(Calculated). |
| ユニプロット: | P22087 |
| RRID: | AB_2838236 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6270, RRID:AB_2838236.
折りたたみ／展開
34 kD nucleolar scleroderma antigen; 34 kDa nucleolar scleroderma antigen; Fbl; FBRL_HUMAN; FIB; FIB1; FLRN; Histone-glutamine methyltransferase; Nop1p; RNA U3 small nucleolar interacting protein 1; RNU3IP1; rRNA 2' O methyltransferase fibrillarin; rRNA 2'-O-methyltransferase fibrillarin;
免疫原
- P22087 FBRL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKPGFSPRGGGFGGRGGFGDRGGRGGRGGFGGGRGRGGGFRGRGRGGGGGGGGGGGGGRGGGGFHSGGNRGRGRGGKRGNQSGKNVMVEPHRHEGVFICRGKEDALVTKNLVPGESVYGEKRVSISEGDDKIEYRAWNPFRSKLAAAILGGVDQIHIKPGAKVLYLGAASGTTVSHVSDIVGPDGLVYAVEFSHRSGRDLINLAKKRTNIIPVIEDARHPHKYRMLIAMVDVIFADVAQPDQTRIVALNAHTFLRNGGHFVISIKANCIDSTASAEAVFASEVKKMQQENMKPQEQLTLEPYERDHAVVVGVYRPPPKVKN
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P22087 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S6 | Phosphorylation | Uniprot | |
| R8 | Methylation | Uniprot | |
| R15 | Methylation | Uniprot | |
| R21 | Methylation | Uniprot | |
| R24 | Methylation | Uniprot | |
| R27 | Methylation | Uniprot | |
| R34 | Methylation | Uniprot | |
| R36 | Methylation | Uniprot | |
| R45 | Methylation | Uniprot | |
| R59 | Methylation | Uniprot | |
| R70 | Methylation | Uniprot | |
| R72 | Methylation | Uniprot | |
| R78 | Methylation | Uniprot | |
| C99 | S-Nitrosylation | Uniprot | |
| K102 | Acetylation | Uniprot | |
| K102 | Sumoylation | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| K109 | Sumoylation | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| S116 | Phosphorylation | Uniprot | |
| Y118 | Phosphorylation | Uniprot | |
| K121 | Acetylation | Uniprot | |
| K121 | Ubiquitination | Uniprot | |
| S124 | Phosphorylation | Uniprot | |
| S126 | Phosphorylation | Uniprot | |
| K131 | Ubiquitination | Uniprot | |
| K143 | Sumoylation | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| K158 | Sumoylation | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| K205 | Acetylation | Uniprot | |
| K205 | Ubiquitination | Uniprot | |
| K206 | Acetylation | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| T208 | Phosphorylation | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| K285 | Ubiquitination | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| T298 | Phosphorylation | Uniprot | |
| Y302 | Phosphorylation | Uniprot | |
| K318 | Ubiquitination | Uniprot |
研究背景
S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate (By similarity). Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity). Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus.
Ubiquitinated. Ubiquitination leads to proteasomal degradation. Deubiquitinated by USP36.
By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).
Acetylated by CREBBP/CBP, preventing methylation of 'Gln-105' of histone H2A (H2AQ104me), without affecting rRNA methylation. Deacetylation by SIRT7 restores methylation of 'Gln-105' of histone H2A (H2AQ104me).
Nucleus>Nucleolus.
Note: Fibrillar region of the nucleolus.
Interacts with NOLC1 (By similarity). Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles that contain SNU13, FBL, NOP5 and NOP56, plus a guide RNA. It is associated with the U3, U8, U13, X and Y small nuclear RNAs. Component of several ribosomal and nucleolar protein complexes. Interacts with PRMT5 and UTP20. Interacts with DDX5 and C1QBP. Interacts with NOL11. Interacts with PIH1D1. Interacts with RRP1B.
Belongs to the methyltransferase superfamily. Fibrillarin family.
研究領域
· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.
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