RAC2 Antibody - #DF6273
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6273, RRID:AB_2838239.
折りたたみ/展開
EN 7; EN-7; EN7; GX; HSPC 022; HSPC022; p21 Rac 2; p21 Rac2; p21-Rac2; p21Rac2; RAC 2; Rac2; RAC2_HUMAN; Ras related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2; Ras related C3 botulinum toxin substrate 2; Ras related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac2); Ras related C3 botulinum toxin substrate 3; Ras-related C3 botulinum toxin substrate 2; Rho family small GTP binding protein Rac 2; Rho family small GTP binding protein Rac2; Small G protein;
免疫原
- P15153 RAC2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDSKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASYENVRAKWFPEVRHHCPSTPIILVGTKLDLRDDKDTIEKLKEKKLAPITYPQGLALAKEIDSVKYLECSALTQRGLKTVFDEAIRAVLCPQPTRQQKRACSLL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P15153 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
K5 | Ubiquitination | Uniprot | |
C6 | S-Nitrosylation | Uniprot | |
Y32 | Phosphorylation | Uniprot | |
Y64 | Phosphorylation | Uniprot | |
K96 | Ubiquitination | Uniprot | |
C105 | S-Nitrosylation | Uniprot | |
K116 | Ubiquitination | Uniprot | |
K123 | Acetylation | Uniprot | |
K123 | Methylation | Uniprot | |
K123 | Ubiquitination | Uniprot | |
K128 | Ubiquitination | Uniprot | |
K130 | Methylation | Uniprot | |
K133 | Ubiquitination | Uniprot | |
K147 | Acetylation | Uniprot | |
K147 | Ubiquitination | Uniprot | |
K153 | Acetylation | Uniprot | |
K153 | Ubiquitination | Uniprot | |
T161 | Phosphorylation | Uniprot | |
R163 | Methylation | Uniprot | |
K166 | Ubiquitination | Uniprot | |
T167 | Phosphorylation | Uniprot | |
C178 | S-Nitrosylation | Uniprot |
研究背景
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase.
(Microbial infection) Glycosylated at Tyr-32 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rac and leads to actin disassembly.
Cytoplasm.
Note: Membrane-associated when activated.
Hematopoietic specific.
Interacts with DOCK2, which may activate it. Interacts with S100A8 and calprotectin (S100A8/9). Found in a complex with SH3RF1, MAP3K7/TAK1, MAP2K7/MKK7, MAPK8IP1/JIP1, MAPK8/JNK1 and MAPK9/JNK2 (By similarity).
Belongs to the small GTPase superfamily. Rho family.
研究領域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Colorectal cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Pancreatic cancer. (View pathway)
· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)
· Human Diseases > Cardiovascular diseases > Viral myocarditis.
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Development > Axon guidance. (View pathway)
· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)
· Organismal Systems > Immune system > B cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Fc epsilon RI signaling pathway. (View pathway)
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
参考文献
Application: WB Species: Rat Sample:
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