MAD1L1 Antibody - #DF6287
| 製品: | MAD1L1 Antibody |
| カタログ: | DF6287 |
| タンパク質の説明: | Rabbit polyclonal antibody to MAD1L1 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Dog |
| 分子量: | 83kDa; 83kD(Calculated). |
| ユニプロット: | Q9Y6D9 |
| RRID: | AB_2838253 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6287, RRID:AB_2838253.
折りたたみ／展開
hMAD1; HsMAD1; MAD1; MAD1 mitotic arrest deficient like 1 (yeast); MAD1-like protein 1; MAD1L1; MD1L1_HUMAN; Mitotic arrest deficient 1; Mitotic arrest deficient 1-like protein 1; Mitotic checkpoint MAD1 protein homolog; Mitotic spindle assembly checkpoint protein MAD1; PIG9; Tax binding protein 181; Tax-binding protein 181; TP53I9; Tumor protein p53 inducible protein 9; TXBP181;
免疫原
- Q9Y6D9 MD1L1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEDLGENTMVLSTLRSLNNFISQRVEGGSGLDISTSAPGSLQMQYQQSMQLEERAEQIRSKSHLIQVEREKMQMELSHKRARVELERAASTSARNYEREVDRNQELLTRIRQLQEREAGAEEKMQEQLERNRQCQQNLDAASKRLREKEDSLAQAGETINALKGRISELQWSVMDQEMRVKRLESEKQELQEQLDLQHKKCQEANQKIQELQASQEARADHEQQIKDLEQKLSLQEQDAAIVKNMKSELVRLPRLERELKQLREESAHLREMRETNGLLQEELEGLQRKLGRQEKMQETLVGLELENERLLAKLQSWERLDQTMGLSIRTPEDLSRFVVELQQRELALKDKNSAVTSSARGLEKARQQLQEELRQVSGQLLEERKKRETHEALARRLQKRVLLLTKERDGMRAILGSYDSELTPAEYSPQLTRRMREAEDMVQKVHSHSAEMEAQLSQALEELGGQKQRADMLEMELKMLKSQSSSAEQSFLFSREEADTLRLKVEELEGERSRLEEEKRMLEAQLERRALQGDYDQSRTKVLHMSLNPTSVARQRLREDHSQLQAECERLRGLLRAMERGGTVPADLEAAAASLPSSKEVAELKKQVESAELKNQRLKEVFQTKIQEFRKACYTLTGYQIDITTENQYRLTSLYAEHPGDCLIFKATSPSGSKMQLLETEFSHTVGELIEVHLRRQDSIPAFLSSLTLELFSRQTVA
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y6D9 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| T8 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| T13 | Phosphorylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| S22 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S29 | Phosphorylation | P53350 (PLK1) | Uniprot |
| K61 | Acetylation | Uniprot | |
| K61 | Sumoylation | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| S62 | Phosphorylation | Uniprot | |
| S77 | Phosphorylation | Uniprot | |
| K79 | Ubiquitination | Uniprot | |
| S90 | Phosphorylation | Uniprot | |
| T91 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| Y96 | Phosphorylation | Uniprot | |
| K123 | Ubiquitination | Uniprot | |
| K143 | Acetylation | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| K163 | Ubiquitination | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| S214 | Phosphorylation | Q13315 (ATM) | Uniprot |
| K231 | Ubiquitination | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| K313 | Ubiquitination | Uniprot | |
| T323 | Phosphorylation | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| K406 | Ubiquitination | Uniprot | |
| T423 | Phosphorylation | Uniprot | |
| Y427 | Phosphorylation | Uniprot | |
| S428 | Phosphorylation | Uniprot | |
| T432 | Phosphorylation | Uniprot | |
| K467 | Ubiquitination | Uniprot | |
| K478 | Acetylation | Uniprot | |
| K481 | Ubiquitination | Uniprot | |
| S484 | Phosphorylation | Uniprot | |
| S485 | Phosphorylation | Uniprot | |
| S486 | Phosphorylation | Uniprot | |
| S490 | Phosphorylation | Uniprot | |
| S494 | Phosphorylation | Uniprot | |
| T500 | Phosphorylation | Uniprot | |
| K504 | Ubiquitination | Uniprot | |
| S513 | Phosphorylation | Uniprot | |
| Y535 | Phosphorylation | Uniprot | |
| S538 | Phosphorylation | Uniprot | |
| S551 | Phosphorylation | Uniprot | |
| S562 | Phosphorylation | Uniprot | |
| T583 | Phosphorylation | Uniprot | |
| S597 | Phosphorylation | Uniprot | |
| S598 | Phosphorylation | Uniprot | |
| K599 | Ubiquitination | Uniprot | |
| S610 | Phosphorylation | Uniprot | |
| K614 | Ubiquitination | Uniprot | |
| K619 | Ubiquitination | Uniprot | |
| T624 | Phosphorylation | Uniprot | |
| K625 | Ubiquitination | Uniprot | |
| Y634 | Phosphorylation | Uniprot | |
| T644 | Phosphorylation | Uniprot | |
| T680 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S699 | Phosphorylation | Uniprot | |
| T708 | Phosphorylation | Uniprot | |
| T716 | Phosphorylation | Uniprot |
研究背景
Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.
Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage.
Nucleus. Chromosome>Centromere>Kinetochore. Nucleus envelope. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle. Cytoplasm>Cytoskeleton>Spindle pole.
Note: Detected at the nucleus envelope during interphase (PubMed:22351768). From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Detected at kinetochores during prometaphase (PubMed:22351768). Colocalizes with NEK2 at the kinetochore (PubMed:14978040). Colocalizes with IK at spindle poles during metaphase and anaphase (PubMed:22351768).
Expressed weakly at G0/G1 and highly at late S and G2/M phase.
Homodimer. Heterodimerizes with MAD2L1 in order to form a tetrameric MAD1L1-MAD2L1 core complex. Perturbation of the original MAD1L1-MAD2L1 structure by the spindle checkpoint may decrease MAD2L1 affinity for MAD1L1. CDC20 can compete with MAD1L1 for MAD2L1 binding, until the attachment and/or tension dampen the checkpoint signal, preventing further release of MAD2L1 on to CDC20. Also able to interact with the BUB1/BUB3 complex. Interacts with NEK2. Interacts with TPR; the interactions occurs in a microtubule-independent manner. Interacts with IK. Interacts with the viral Tax protein.
Belongs to the MAD1 family.
研究領域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
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