RHEB Antibody - #DF6299

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製品: RHEB Antibody
カタログ: DF6299
タンパク質の説明: Rabbit polyclonal antibody to RHEB
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 20kDa; 20kD(Calculated).
ユニプロット: Q15382
RRID: AB_2838265

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(83%)
クローナリティ:
Polyclonal
特異性:
RHEB Antibody detects endogenous levels of total RHEB.
RRID:
AB_2838265
引用形式: Affinity Biosciences Cat# DF6299, RRID:AB_2838265.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Ras homolog enriched in brain 2, formerly; GTP binding protein Rheb; GTP-binding protein Rheb; MGC111559; Ras homolog enriched in brain 2; Ras homolog enriched in brain; RHEB 2; Rheb; RHEB_HUMAN; RHEB2; RHEB2, formerly;

免疫原

免疫原:
Uniprot:

Q15382(RHEB_HUMAN) >>Visit HPA database.

遺伝子(ID):
RHEB(6009)
発現特異性:
Q15382 RHEB_HUMAN:

Ubiquitous. Highest levels observed in skeletal and cardiac muscle.

タンパク質の説明:
Rheb (Ras Homolog Enriched in Brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure (1). While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Like other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway (2). Biochemical and genetic studies demonstrate that Rheb has an important role in regulating the insulin/TOR signaling pathway (3-6). The mammalian target of rapamycin, mTOR, is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with translation and cell growth. The tuberin/hamartin (TSC2/TSC1) complex inhibits mTOR activity indirectly by inhibiting Rheb via tuberin's GAP activity (7).
タンパク質配列:
MPQSKSRKIAILGYRSVGKSSLTIQFVEGQFVDSYDPTIENTFTKLITVNGQEYHLQLVDTAGQDEYSIFPQTYSIDINGYILVYSVTSIKSFEVIKVIHGKLLDMVGKVQIPIMLVGNKKDLHMERVISYEEGKALAESWNAAFLESSAKENQTAVDVFRRIILEAEKMDGAASQGKSSCSVM

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Rabbit
100
Xenopus
83
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15382 基板として

Site PTM Type Enzyme
Y14 Phosphorylation
S16 Phosphorylation
K102 Ubiquitination
K109 Ubiquitination
K120 Ubiquitination
K121 Ubiquitination
S130 Phosphorylation Q8IW41 (MAPKAPK5)
K135 Ubiquitination
S140 Phosphorylation
K151 Ubiquitination
K169 Ubiquitination
S175 Phosphorylation

PTMs - Q15382 酵素として

Substrate Site Source
P04049-1 (RAF1) S338 Uniprot
P15056 (BRAF) S446 Uniprot

研究背景

機能:

Activates the protein kinase activity of mTORC1, and thereby plays a role in the regulation of apoptosis. Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Has low intrinsic GTPase activity.

PTMs:

Farnesylation is important for efficiently activating mTORC1-mediated signaling.

Phosphorylation by MAPKAPK5 impairs GTP-binding and inactivation.

細胞の位置付け:

Endomembrane system>Lipid-anchor>Cytoplasmic side. Golgi apparatus membrane>Lipid-anchor>Cytoplasmic side. Cytoplasm>Cytosol. Endoplasmic reticulum membrane>Lipid-anchor>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitous. Highest levels observed in skeletal and cardiac muscle.

サブユニット構造:

Binds to mTORC1 in a guanyl nucleotide-independent manner. Interacts directly with MTOR, MLST8 and RPTOR. Interacts with TSC2. Interacts (when prenylated) with PDE6D; this promotes release from membranes.

タンパク質ファミリー:

Belongs to the small GTPase superfamily. Rheb family.

研究領域

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

参考文献

1). An Injectable Sustained Release Hydrogel of Hyaluronic Acid Loaded with β-Ecdysterone Ameliorates Cartilage Damage in Osteoarthritis via Activating Autophagy. Advanced Therapeutics, 2023 [IF=4.6]

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