FAAH Antibody - #DF6308

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製品: FAAH Antibody
カタログ: DF6308
タンパク質の説明: Rabbit polyclonal antibody to FAAH
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat, Monkey
予測: Pig, Horse, Rabbit, Dog
分子量: 63kDa; 63kD(Calculated).
ユニプロット: O00519
RRID: AB_2838274

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MS Report
HPLC Report
MSDS
説明書
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IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat,Monkey
予測:
Pig(88%), Horse(100%), Rabbit(100%), Dog(89%)
クローナリティ:
Polyclonal
特異性:
FAAH Antibody detects endogenous levels of total FAAH.
RRID:
AB_2838274
引用形式: Affinity Biosciences Cat# DF6308, RRID:AB_2838274.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Anandamide amidohydrolase 1; Anandamide amidohydrolase; FA2H; FAAH; FAAH-1; FAAH1_HUMAN; Fatty acid 2-hydroxylase; Fatty acid alpha-hydroxylase; Fatty acid amide hydrolase; Fatty-acid amide hydrolase 1; MGC102823; MGC138146; Oleamide hydrolase 1; Oleamide hydrolase;

免疫原

免疫原:
Uniprot:

O00519(FAAH1_HUMAN) >>Visit HPA database.

遺伝子(ID):
FAAH(2166)
発現特異性:
O00519 FAAH1_HUMAN:

Highly expressed in the brain, small intestine, pancreas, skeletal muscle and testis. Also expressed in the kidney, liver, lung, placenta and prostate.

タンパク質の説明:
Endogenous cannabinoids have been implicated in addictive behaviors and drug abuse (1). Fatty-acid amide hydrolase 1 (FAAH1) is a plasma membrane-bound hydrolase that converts oleamide to oleic acid (2). This hydrolase also converts the cannabinoid anandamide, the endogenous ligand for the CB1 cannabinoid receptor, to arachidonic acid, suggesting a role in fatty-acid amide inactivation (2). Mice lacking FAAH1 have significantly higher levels of anandamide in the brain and show decreased sensitivity to pain, further indicating a role for FAAH1 in the regulation of endocannabinoid signaling in vivo (3). FAAH1 null mice also demonstrate an increased preference for alcohol and an increased voluntary uptake of alcohol as compared to wild-type mice, indicating a role of FAAH1 in modulating addictive behaviors (1).
タンパク質配列:
MVQYELWAALPGASGVALACCFVAAAVALRWSGRRTARGAVVRARQRQRAGLENMDRAAQRFRLQNPDLDSEALLALPLPQLVQKLHSRELAPEAVLFTYVGKAWEVNKGTNCVTSYLADCETQLSQAPRQGLLYGVPVSLKECFTYKGQDSTLGLSLNEGVPAECDSVVVHVLKLQGAVPFVHTNVPQSMFSYDCSNPLFGQTVNPWKSSKSPGGSSGGEGALIGSGGSPLGLGTDIGGSIRFPSSFCGICGLKPTGNRLSKSGLKGCVYGQEAVRLSVGPMARDVESLALCLRALLCEDMFRLDPTVPPLPFREEVYTSSQPLRVGYYETDNYTMPSPAMRRAVLETKQSLEAAGHTLVPFLPSNIPHALETLSTGGLFSDGGHTFLQNFKGDFVDPCLGDLVSILKLPQWLKGLLAFLVKPLLPRLSAFLSNMKSRSAGKLWELQHEIEVYRKTVIAQWRALDLDVVLTPMLAPALDLNAPGRATGAVSYTMLYNCLDFPAGVVPVTTVTAEDEAQMEHYRGYFGDIWDKMLQKGMKKSVGLPVAVQCVALPWQEELCLRFMREVERLMTPEKQSS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
100
Dog
89
Pig
88
Bovine
67
Sheep
67
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00519 基板として

Site PTM Type Enzyme
S88 Phosphorylation
T99 Phosphorylation
S241 Phosphorylation
S289 Phosphorylation
Y329 Phosphorylation
Y335 Phosphorylation
K423 Ubiquitination
S430 Phosphorylation
S434 Phosphorylation
S440 Phosphorylation
Y454 Phosphorylation

研究背景

機能:

Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates.

細胞の位置付け:

Endomembrane system>Single-pass membrane protein. Cytoplasm>Cytoskeleton.
Note: Seems to be attached to intracellular membranes and a portion of the cytoskeletal network.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highly expressed in the brain, small intestine, pancreas, skeletal muscle and testis. Also expressed in the kidney, liver, lung, placenta and prostate.

サブユニット構造:

Homodimer.

タンパク質ファミリー:

Belongs to the amidase family.

研究領域

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

参考文献

1). MicroRNA miR-1275 coordinately regulates AEA/LPA signals via targeting FAAH in lipid metabolism reprogramming of gastric cancer. Cell Death & Disease, 2023 (PubMed: 36702852) [IF=9.0]

Application: WB    Species: Mouse    Sample: GC tissues

Fig. 1: FAAH is highly expressed in GC tissues.A Clustered heatmap. Each row represents a tissue sample, and each column represents a coding gene. B Volcano plots. The red points on the right indicate the differentially upregulated genes with statistical significance while the red points on the left indicate the downregulated genes. Differential expression of FAAH in tissues from C TCGA database and D Oncomine database. E Differential expression analysis of FAAH in 4 GC subtypes (data from TCGA). F mRNA levels of FAAH in 96 pairs of GC tissues. G Protein levels of FAAH in 20 pairs of GC tissues. *p 

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