SERPINB5 Antibody - #DF6313

(5)  
製品: SERPINB5 Antibody
カタログ: DF6313
タンパク質の説明: Rabbit polyclonal antibody to SERPINB5
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 42kDa; 42kD(Calculated).
ユニプロット: P36952
RRID: AB_2838279

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(91%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
SERPINB5 Antibody detects endogenous levels of total SERPINB5.
RRID:
AB_2838279
引用形式: Affinity Biosciences Cat# DF6313, RRID:AB_2838279.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Maspin; Ovalbumin; Peptidase inhibitor 5; PI-5; PI5; protease inhibitor 5 (maspin); Protease inhibitor 5; Serine (or cysteine) peptidase inhibitor, clade B, member 5; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5; Serpin B5; serpin family B member 5; Serpin peptidase inhibitor; Serpin peptidase inhibitor, clade B (ovalbumin), member 5; Serpinb5; SPB5_HUMAN; Spi5; Spi7;

免疫原

免疫原:
Uniprot:

P36952(SPB5_HUMAN) >>Visit HPA database.

遺伝子(ID):
SERPINB5(5268)
発現特異性:
P36952 SPB5_HUMAN:

Normal mammary epithelial cells.

タンパク質の説明:
Maspin (SERPINB5) was discovered as a mammary tumor suppressor that is expressed in normal mammary epithelium but lost in most breast cancer cell lines (1). While maspin is related to the serpin family of serine protease inhibitors, it may not function as a protease inhibitor (2). It plays an essential role in embryonic development through critical roles in cell adhesion (3). While the precise mechanism of maspin signaling is unclear (4), the tumor suppressing activity of maspin has been attributed to its ability to inhibit cell invasion/metastasis (5,6) and angiogenesis (7), while promoting apoptosis (8). Nuclear translocation of active IKKα has been shown to repress maspin transcription and promote prostate cancer metastasis (9).
タンパク質配列:
MDALQLANSAFAVDLFKQLCEKEPLGNVLFSPICLSTSLSLAQVGAKGDTANEIGQVLHFENVKDVPFGFQTVTSDVNKLSSFYSLKLIKRLYVDKSLNLSTEFISSTKRPYAKELETVDFKDKLEETKGQINNSIKDLTDGHFENILADNSVNDQTKILVVNAAYFVGKWMKKFSESETKECPFRVNKTDTKPVQMMNMEATFCMGNIDSINCKIIELPFQNKHLSMFILLPKDVEDESTGLEKIEKQLNSESLSQWTNPSTMANAKVKLSIPKFKVEKMIDPKACLENLGLKHIFSEDTSDFSGMSETKGVALSNVIHKVCLEITEDGGDSIEVPGARILQHKDELNADHPFIYIIRHNKTRNIIFFGKFCSP

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
91
Chicken
64
Xenopus
45
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P36952 基板として

Site PTM Type Enzyme
S9 Phosphorylation
T50 Phosphorylation
K64 Ubiquitination
K79 Ubiquitination
S85 Phosphorylation
Y93 Phosphorylation
S97 Phosphorylation
K109 Ubiquitination
K114 Acetylation
K114 Ubiquitination
T118 Phosphorylation
K129 Ubiquitination
S135 Phosphorylation
K137 Ubiquitination
T157 Phosphorylation
S240 Phosphorylation
T241 Phosphorylation
K245 Ubiquitination
K248 Ubiquitination
K275 Ubiquitination
K277 Acetylation
K280 Acetylation
K285 Ubiquitination
S298 Phosphorylation
S302 Phosphorylation
T310 Phosphorylation
K311 Ubiquitination
S316 Phosphorylation
K345 Ubiquitination
Y356 Phosphorylation

研究背景

機能:

Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

細胞の位置付け:

Secreted>Extracellular space.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Normal mammary epithelial cells.

サブユニット構造:

Interacts with IRF6.

タンパク質ファミリー:

Belongs to the serpin family. Ov-serpin subfamily.

研究領域

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

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