CD86 Antibody - #DF6332

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製品: CD86 Antibody
カタログ: DF6332
タンパク質の説明: Rabbit polyclonal antibody to CD86
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse
分子量: 38kDa,60-85kD; 38kD(Calculated).
ユニプロット: P42081
RRID: AB_2838296

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
クローナリティ:
Polyclonal
特異性:
CD86 Antibody detects endogenous levels of total CD86.
RRID:
AB_2838296
引用形式: Affinity Biosciences Cat# DF6332, RRID:AB_2838296.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Activation B7-2 antigen 3; Activation B7-2 antigen; B-lymphocyte activation antigen B7-2 2; B-lymphocyte activation antigen B7-2; B7 2; B7; B7-2; B7.2; B70; B72 antigen; BU63; CD28 antigen ligand 2 2; CD28 antigen ligand 2; Cd28l2; CD28LG2; CD86; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CD86 antigen; CD86 molecule; CD86_HUMAN; CLS1; CTLA-4 counter-receptor B7.2 2, 3; CTLA-4 counter-receptor B7.2; Early T-cell costimulatory molecule 1; ETC-1; FUN 1; FUN-1; LAB72; Ly-58; Ly58; MB7; MB7-2; Membrane glycoprotein; MGC34413; T-lymphocyte activation antigen CD86; TS/A-2;

免疫原

免疫原:
Uniprot:

P42081(CD86_HUMAN) >>Visit HPA database.

遺伝子(ID):
CD86(942)
発現特異性:
P42081 CD86_HUMAN:

Expressed by activated B-lymphocytes and monocytes.

タンパク質の説明:
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]
タンパク質配列:
MDPQCTMGLSNILFVMAFLLSGAAPLKIQAYFNETADLPCQFANSQNQSLSELVVFWQDQENLVLNEVYLGKEKFDSVHSKYMGRTSFDSDSWTLRLHNLQIKDKGLYQCIIHHKKPTGMIRIHQMNSELSVLANFSQPEIVPISNITENVYINLTCSSIHGYPEPKKMSVLLRTKNSTIEYDGVMQKSQDNVTELYDVSISLSVSFPDVTSNMTIFCILETDKTRLLSSPFSIELEDPQPPPDHIPWITAVLPTVIICVMVFCLILWKWKKKKRPRNSYKCGTNTMEREESEQTKKREKIHIPERSDEAQRVFKSSKTSSCDKSDTCF

PTMs - P42081 基板として

Site PTM Type Enzyme
S77 Phosphorylation
S80 Phosphorylation
Y108 Phosphorylation
T175 Phosphorylation
K315 Acetylation

研究背景

機能:

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.

(Microbial infection) Acts as a receptor for adenovirus subgroup B.

PTMs:

Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.

細胞の位置付け:

Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed by activated B-lymphocytes and monocytes.

サブユニット構造:

Homodimer. Interacts with MARCH8.

(Microbial infection) Interacts with adenovirus subgroup b fiber protein.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Type I diabetes mellitus.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Immune diseases > Autoimmune thyroid disease.

· Human Diseases > Immune diseases > Systemic lupus erythematosus.

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Human Diseases > Immune diseases > Allograft rejection.

· Human Diseases > Immune diseases > Graft-versus-host disease.

· Human Diseases > Cardiovascular diseases > Viral myocarditis.

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Intestinal immune network for IgA production.   (View pathway)

参考文献

1). Toxin-Enabled “On-Demand” Liposomes for Enhanced Phototherapy to Treat and Protect against Methicillin-Resistant Staphylococcus aureus Infection. Small, 2022 (PubMed: 35859534) [IF=13.3]
2). Bacterial Toxin‐Responsive Biomimetic Nanobubbles for Precision Photodynamic Therapy against Bacterial Infections. Advanced Healthcare Materials, 2022 (PubMed: 35836329) [IF=10.0]
3). Ganoderma lucidum polysaccharide hydrogel accelerates diabetic wound healing by regulating macrophage polarization. International journal of biological macromolecules, 2024 (PubMed: 38266851) [IF=8.2]
4). Multimodal imaging and photothermal synergistic immunotherapy of retinoblastoma with tuftsin-loaded carbonized MOF nanoparticles. DRUG DELIVERY, 2022 (PubMed: 35642917) [IF=6.0]

Application: WB    Species: Mice    Sample: Y79 tumors

Figure 11. (A, B) The contents of CD86 in Y79 tumors after different treatments. (C) TNF-α levels in Y79 tumors after different treatments (**p<.01).
5). Recombinant fibroblast growth factor 4 ameliorates axonal regeneration and functional recovery in acute spinal cord injury through altering microglia/macrophage phenotype. International immunopharmacology, 2024 (PubMed: 38728880) [IF=5.6]
6). Isosteviol reduces the acute inflammatory response after burns by upregulating MMP9 in macrophages leading to M2 polarization. International Immunopharmacology, 2022 (PubMed: 35176589) [IF=5.6]
7). Zanubrutinib ameliorates lipopolysaccharide-induced acute lung injury via regulating macrophage polarization. International Immunopharmacology, 2022 (PubMed: 35973369) [IF=5.6]
8). miR-146a-5p enhances embryo survival in unexplained recurrent spontaneous abortion by promoting M2 polarization of decidual macrophages. International Immunopharmacology, 2022 (PubMed: 35764020) [IF=5.6]
9). Vascular endothelial growth factor A is a potential prognostic biomarker and correlates with immune cell infiltration in hepatocellular carcinoma. Journal of Cellular and Molecular Medicine, 2023 (PubMed: 36729917) [IF=5.3]
10). Contribution of Oxidative Stress Induced by Sonodynamic Therapy to the Calcium Homeostasis Imbalance Enhances Macrophage Infiltration in Glioma Cells. Cancers, 2022 (PubMed: 35454942) [IF=5.2]

Application: IF/ICC    Species: Mouse    Sample: brain

Figure 8. (A) GL261 glioma-bearing C57BL/6 to undergo xenograft tumor and brain treatment and MRI tests on days 0 and 24. (B,D) At the end of the experiment (30 days), the mouse brains were made into sections for immunofluorescence staining (CD86 and CD206); (C,E) analysis of the immunofluorescence intensity showed that the SG group induced more CD86 expression in the gliomas than CD206 expression.
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