RASSF1 Antibody - #DF6335
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| 製品: | RASSF1 Antibody |
| カタログ: | DF6335 |
| タンパク質の説明: | Rabbit polyclonal antibody to RASSF1 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 39kDa; 39kD(Calculated). |
| ユニプロット: | Q9NS23 |
| RRID: | AB_2838299 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6335, RRID:AB_2838299.
折りたたみ／展開
123F2; Cardiac specific ras association domain family 1 protein; cardiac-specific ras association domain family 1 protein, 123F2; NORE2A; OTTHUMP00000195503; OTTHUMP00000195504; OTTHUMP00000195505; OTTHUMP00000195507; Pancreas specific ras association domain family 1 protein; Ras association (RalGDS/AF 6) domain family member 1; Ras association domain-containing protein 1; RASF1_HUMAN; Rassf1; RASSF1A; RDA32; REH3P21; Tumor suppressor protein RDA32; WUGSC:H_LUCA12.5;
免疫原
Isoform A and isoform C are ubiquitously expressed in all tissues tested, however isoform A is absent in many corresponding cancer cell lines. Isoform B is mainly expressed in hematopoietic cells.
- Q9NS23 RASF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGEPELIELRELAPAGRAGKGRTRLERANALRIARGTACNPTRQLVPGRGHRFQPAGPATHTWCDLCGDFIWGVVRKGLQCARLSADCKFTCHYRCRALVCLDCCGPRDLGWEPAVERDTNVDEPVEWETPDLSQAEIEQKIKEYNAQINSNLFMSLNKDGSYTGFIKVQLKLVRPVSVPSSKKPPSLQDARRGPGRGTSVRRRTSFYLPKDAVKHLHVLSRTRAREVIEALLRKFLVVDDPRKFALFERAERHGQVYLRKLLDDEQPLRLRLLAGPSDKALSFVLKENDSGEVNWDAFSMPELHNFLRILQREEEEHLRQILQKYSYCRQKIQEALHACPLG
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NS23 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| R18 | Methylation | Uniprot | |
| K21 | Acetylation | Uniprot | |
| R36 | Methylation | Uniprot | |
| S135 | Phosphorylation | Q13315 (ATM) | Uniprot |
| K142 | Ubiquitination | Uniprot | |
| K185 | Ubiquitination | Uniprot | |
| S188 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| R198 | Methylation | Uniprot | |
| T206 | Phosphorylation | O14965 (AURKA) | Uniprot |
| S207 | Phosphorylation | Q96GD4 (AURKB) , P11802 (CDK4) , O14965 (AURKA) | Uniprot |
| K216 | Ubiquitination | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| T224 | Phosphorylation | Uniprot | |
| K236 | Ubiquitination | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| Y259 | Phosphorylation | Uniprot | |
| K262 | Ubiquitination | Uniprot | |
| K281 | Ubiquitination | Uniprot | |
| K326 | Ubiquitination | Uniprot |
研究背景
Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage.
Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle. Cytoplasm>Cytoskeleton>Spindle pole. Nucleus.
Note: Localizes to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late telophase in cells. Colocalizes with MDM2 in the nucleus.
Nucleus.
Note: Predominantly nuclear.
Isoform A and isoform C are ubiquitously expressed in all tissues tested, however isoform A is absent in many corresponding cancer cell lines. Isoform B is mainly expressed in hematopoietic cells.
Interacts with MAP1S. Interacts with XPA. Binds to the N-terminal of CDC20 during prometaphase. Binds to STK3/MST2 and STK4/MST1. Isoform A interacts with MOAP1. Isoform A interacts with E4F1. Recruited to the TNFRSF1A and TNFRSF10A complexes in response to their respective cognate ligand, after internalization. Can self-associate. Isoform A interacts with RSSF5 and probably associates with HRAS via a RSSF1 isoform A-RSSF5 heterodimer. Part of a complex with MDM2, DAXX, RASSF1 and USP7. Isoform A interacts (via C-terminus) with DAXX (via N-terminus); the interaction is independent of MDM2 and TP53. Isoform C or isoform H interacts (via N-terminus) with DAXX. Isoform A interacts (via N-terminus) with MDM2 (via C-terminus); the interaction is independent of TP53. Interacts with ECM2 (By similarity).
研究領域
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species. (View pathway)
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Human Diseases > Cancers: Specific types > Bladder cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Non-small cell lung cancer. (View pathway)
参考文献
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